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PSGL-1 elisa kit :: Mouse P-selectin glycoprotein ligand 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS731419
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

P-selectin glycoprotein ligand 1 (PSGL-1), ELISA Kit

★Popular Item★
 Also Known As   

Mouse P-selectin glycoprotein ligand 1 ELISA Kit

 Product Gene Name   

PSGL-1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    600738
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of PSGL-1. No significant cross-reactivity or interference between PSGL-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PSGL-1 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    0.1 ng/mL.
 Intended Uses    This PSGL-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse PSGL-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PSGL-1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PSGL-1 purchase    MBS731419 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the P-selectin glycoprotein ligand 1 (PSGL-1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PSGL-1, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS731419 kit is based on PSGL-1 antibody-PSGL-1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PSGL-1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PSGL-1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PSGL-1 elisa kit

   Principle of the Assay: PSGL-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PSGL-1 antibody and an PSGL-1-HRP conjugate. The assay sample and buffer are incubated together with PSGL-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PSGL-1 concentration since PSGL-1 from samples and PSGL-1-HRP conjugate compete for the anti-PSGL-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by PSGL-1 from the sample, fewer sites are left to bind PSGL-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PSGL-1 concentration in each sample is interpolated from this standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of PSGL-1 elisa kit    PSGL-1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS731419. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PSGL-1. It may not necessarily be applicable to this product.
 NCBI GI #    331284236
 NCBI GeneID    6404
 NCBI Accession #    NP_002997.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_003006.4 [Other Products]
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 UniProt Secondary Accession #    Q12775; Q6GTW7; Q8N7J7; A8K2Y0; B4DQC3; B7Z5C7; J3KMX6 [Other Products]
 UniProt Related Accession #    Q14242 [Other Products]
 Molecular Weight    44,648 Da
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 NCBI Official Full Name    P-selectin glycoprotein ligand 1 isoform 2
 NCBI Official Synonym Full Names    selectin P ligand
 NCBI Official Symbol    SELPLG [Similar Products]
 NCBI Official Synonym Symbols   
CLA; CD162; PSGL1; PSGL-1
[Similar Products]
 NCBI Protein Information    P-selectin glycoprotein ligand 1; cutaneous lymphocyte-associated associated antigen
 UniProt Protein Name    P-selectin glycoprotein ligand 1
 UniProt Synonym Protein Names   
Selectin P ligand; CD_antigen: CD162
 Protein Family    P-selectin glycoprotein ligand
 UniProt Gene Name    SELPLG [Similar Products]
 UniProt Synonym Gene Names    PSGL-1 [Similar Products]
 UniProt Entry Name    SELPL_HUMAN
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 NCBI Summary for PSGL-1    This gene encodes a glycoprotein that functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2011]
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 UniProt Comments for PSGL-1    SELPLG: A SLe(x)-type glycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. PSGL1 is critical for the initial leukocyte capture.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q24

Cellular Component: membrane; integral to plasma membrane; integral to membrane; plasma membrane

Molecular Function: protein binding; receptor binding

Biological Process: cell adhesion; blood coagulation; leukocyte tethering or rolling; leukocyte adhesive activation; leukocyte migration
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 Research Articles on PSGL-1    1. Specific phosphorylations on 130 and 153 tyrosine residues of p85-bound Rho-GDP dissociation inhibitor-2 are pivotal for P-selectin glycoprotein ligand-1-induced beta1 integrin-mediated lymphocyte adhesion to vascular cell adhesion molecule-1.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PSGL-1 elisa kitPathways associated with PSGL-1 elisa kit
 Reference Product  PubMed Publications
 SELP elisa kit  >5 publications with PSGL-1 and SELP
 SYK elisa kit  >1 publications with PSGL-1 and SYK
 SELL elisa kit  >1 publications with PSGL-1 and SELL
 Products by Pathway  Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Staphylococcus Aureus Infection Pathway antibodies  Staphylococcus Aureus Infection Pathway Diagram
 Staphylococcus Aureus Infection Pathway antibodies  Staphylococcus Aureus Infection Pathway Diagram
 Amb2 Integrin Signaling Pathway antibodies  Amb2 Integrin Signaling Pathway Diagram
Diseases associated with PSGL-1 elisa kitOrgans/Tissues associated with PSGL-1 elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >7 publications with PSGL-1 and Cardiovascular Diseases
 Heart Diseases Antibodies  >4 publications with PSGL-1 and Heart Diseases
 Atherosclerosis Antibodies  >4 publications with PSGL-1 and Atherosclerosis
 Inflammation Antibodies  >2 publications with PSGL-1 and Inflammation
 Hypertension Antibodies  >1 publications with PSGL-1 and Hypertension
 Diabetes Mellitus, Type 2 Antibodies  >1 publications with PSGL-1 and Diabetes Mellitus, Type 2
 Myocardial Infarction Antibodies  >1 publications with PSGL-1 and Myocardial Infarction
 Neoplasms, Experimental Antibodies  >1 publications with PSGL-1 and Neoplasms, Experimental
 Necrosis Antibodies  >1 publications with PSGL-1 and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >11 publications with PSGL-1 and Blood
 Bone Antibodies  >2 publications with PSGL-1 and Bone
 Heart Antibodies  >2 publications with PSGL-1 and Heart
 Bone Marrow Antibodies  >2 publications with PSGL-1 and Bone Marrow
 Placenta Antibodies  >2 publications with PSGL-1 and Placenta
 Embryonic Tissue Antibodies  >2 publications with PSGL-1 and Embryonic Tissue
 Lung Antibodies  >1 publications with PSGL-1 and Lung
 Spleen Antibodies  >1 publications with PSGL-1 and Spleen
 Brain Antibodies  >1 publications with PSGL-1 and Brain
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