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PAP elisa kit :: Mouse PAP ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2512896 PAP elisa kit
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

PAP, ELISA Kit

 Also Known As   

Mouse PAP (Plasmin-Antiplasmin Complex) ELISA Kit

 Product Gene Name   

PAP elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 MBS2512896 COA    COA PDF
 3D Structure    ModBase 3D Structure for Q71T77
 Species Reactivity    Mouse
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 Specificity    This kit recognizes natural and recombinantMousePAP. No significant cross-reactivity or interference between MousePAP and analogues was observed.
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 Samples    Serum, plasma and other biological fluids.
 Assay Type    Sandwich (Quantitative)
 Detection Range    3.125-200ng/mL
 Sensitivity    1.875ng/mL
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 Preparation and Storage    Store at 4 degree C.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of PAP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PAP purchase    MBS2512896 is a ready-to-use microwell, strip plate Sandwich (Quantitative) ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the PAP, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 3.125-200ng/mL in biological research samples containing PAP, with an estimated sensitivity of 1.875ng/mL. The ELISA analytical biochemical technique of the MBS2512896 kit is based on PAP antibody-PAP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PAP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PAP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for PAP elisa kit

   Intended Uses: This ELISA kit applies to the invitro quantitative determination of MousePAPconcentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PAP. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PAPand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PAP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PAP.You can calculate the concentration of PAPin the samples by comparing the OD of the samples to the standard curve.
Sample Manual Insert of MBS2512896
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NCBI/Uniprot data below describe general gene information for PAP. It may not necessarily be applicable to this product.
 NCBI GI #    46401714
 NCBI GeneID    2777457
 NCBI Accession #    YP_006560.1 [Other Products]
 NCBI GenBank Nucleotide #    NC_005856.1 [Other Products]
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 UniProt Primary Accession #    Q71T77 [Other Products]
 UniProt Related Accession #    Q71T77 [Other Products]
 Molecular Weight    19,219 Da
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 NCBI Official Full Name    Pap
 NCBI Official Symbol    pap [Similar Products]
 NCBI Protein Information    putative P1 acid phosphatase; Pap
 UniProt Protein Name    Pap
 UniProt Synonym Protein Names   
Pap
 Protein Family    Papain
 UniProt Gene Name    pap [Similar Products]
 UniProt Entry Name    Q71T77_BPP1
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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