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| Product Name
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PAP, ELISA Kit ★Popular Item★
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| Also Known As
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Mouse PAP (Plasmin-Antiplasmin Complex) ELISA Kit
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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| Sample Manual Insert
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| Request for Current Manual Insert
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| MBS2512896 COA
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| 3D Structure
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ModBase 3D Structure for Q71T77
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| Species Reactivity
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Mouse
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| Specificity
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This kit recognizes natural and recombinantMousePAP. No significant cross-reactivity or interference between MousePAP and analogues was observed.
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| Samples
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Serum, plasma and other biological fluids.
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| Assay Type
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Sandwich (Quantitative)
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| Detection Range
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3.125-200ng/mL
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| Sensitivity
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1.875ng/mL
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| Preparation and Storage
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Store at 4 degree C.
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| ISO Certification
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Manufactured in an ISO 9001:2008 Certified Laboratory.
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| Product Note
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Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
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| Other Notes
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Small volumes of PAP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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| Searchable Terms for PAP purchase
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MBS2512896 is a ready-to-use microwell, strip plate Sandwich (Quantitative) ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the PAP, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 3.125-200ng/mL in biological research samples containing PAP, with an estimated sensitivity of 1.875ng/mL. The ELISA analytical biochemical technique of the MBS2512896 kit is based on PAP antibody-PAP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PAP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PAP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PAP elisa kit
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Intended Uses: This ELISA kit applies to the invitro quantitative determination of MousePAPconcentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PAP. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PAPand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PAP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PAP.You can calculate the concentration of PAPin the samples by comparing the OD of the samples to the standard curve.
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| Typical Testing Data/Standard Curve (for reference only) of PAP elisa kit
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| Sample Manual Insert of MBS2512896. Click to request current manual
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| NCBI/Uniprot data below describe general gene information for PAP. It may not necessarily be applicable to this product. |
| NCBI GI #
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46401714
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| NCBI GeneID
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2777457
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| NCBI Accession #
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YP_006560.1
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[Other Products]
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| NCBI GenBank Nucleotide #
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NC_005856.1
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[Other Products]
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| UniProt Primary Accession #
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Q71T77
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[Other Products]
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| UniProt Related Accession #
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Q71T77
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[Other Products]
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| Molecular Weight
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19,219 Da
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| NCBI Official Full Name
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Pap
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| NCBI Official Symbol
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pap
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[Similar Products]
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| NCBI Protein Information
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putative P1 acid phosphatase; Pap
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| UniProt Protein Name
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Pap
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| UniProt Synonym Protein Names
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Pap
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| Protein Family
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Papain
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| UniProt Gene Name
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pap
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[Similar Products]
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| UniProt Entry Name
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Q71T77_BPP1
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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ELISA Kit 
