Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow PDGFB,PDGFA rightarrow Mouse PDGFB,PDGFA  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Microtubule Associated Protein Tau (MAPtau) ELISA Kit
 • Alpha-amylase inhibitor HOE-467A Recombinant Protein
 • FGF21 ELISA Kit
 • ALDH18A1 Antibody
 • Glutamic-Pyruvate Transaminase (GPT) Recombinant Protein
 • Aggrecan (AGC) ELISA Kit
 • ITCH Antibody
 • Lyve-1 Antibody
 • SARM (SARM1) Antibody
 • Pentagastrin Peptide
 • JAK1 Antibody
 • Palladin (PALLD) Antibody
 • ataxia telangiectasia and Rad3 related (ATR) ELISA Kit
 • Serotonin Antibody
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

PDGFB,PDGFA elisa kit :: Mouse PDGF-AB ELISA Kit

Scan QR to view Datasheet Catalog #    MBS175775
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96-Strip-Wells  /  $455 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

PDGF-AB (PDGFB,PDGFA), ELISA Kit

 Also Known As   

Mouse PDGF-AB PicoKine ELISA Kit

 Product Synonym Names    Platelet-derived growth factor subunit B,Platelet-derived growth factor subunit A; platelet-derived growth factor beta polypeptide,platelet-derived growth factor alpha polypeptide; PDGF A chain; PDGF B chain; PDGF1; PDGF2; PDGFA; PDGFB; Platelet derived growth factor AB heterodimer
 Product Gene Name   

PDGFB,PDGFA elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for P31240
 Species Reactivity    Mouse
Section Bar
 Specificity    Natural and recombinant mouse PDGF-AB
Section Bar
 Samples    Cell culture supernates, serum and plasma (heparin, EDTA)
 Assay Type    Sandwich
 Detection Range    31.2pg/ml-2000pg/ml
 Sensitivity    < 3pg/ml
Section Bar
 Preparation and Storage    Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PDGFB,PDGFA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PDGFB,PDGFA purchase    MBS175775 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the PDGF-AB (PDGFB,PDGFA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.2pg/ml-2000pg/ml in biological research samples containing PDGFB,PDGFA, with an estimated sensitivity of < 3pg/ml. The ELISA analytical biochemical technique of the MBS175775 kit is based on PDGFB,PDGFA antibody-PDGFB,PDGFA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PDGFB,PDGFA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PDGFB,PDGFA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Cell culture supernates, serum and plasma (heparin, EDTA). Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for PDGFB,PDGFA elisa kit

   Principle of the assay: mouse PDGF-AB ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for PDGF-AB has been precoated onto 96-well plates. Standards (E.coli,(S87-T211)+(S82-T190)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PDGF-AB is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse PDGF-AB amount of sample captured in plate.
Background: The platelet-derived growth factor (PDGF) is a mitogen derived from human platelets consisting of two related polypeptides termed A and B chains.1 The genes for PDGF A chain, B chain/c-sis, and the PDGF receptor areexpressed in human malignant glioma cell lines.2 Normal human endothelial cells in culture express the B chain ofPDGF, and that endothelial-derived PDGF B chain is synthesized as a predicted precursor polypeptide of Mr 27,281.3 The entire B chain of PDGF is highly (96%) homologous to a portion of p28sis, the transforming protein ofsimian sarcoma virus (SSV). It has been suggested that p28sis exerts its transforming potential by mimicking the growth promoting activity of PDGF and stimulating the cell in an autocrine manner.1 PDGF A-chain precursorpolypeptide is assigned to the proximal long arm of chromosome 7, band q11.23.4 The human homolog (PDGFB-chain/c-sis) of the transforming gene of simian sarcoma virus is assigned to chromosome 22.5
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of PDGFB,PDGFA elisa kit    Mouse PDGF-AB PicoKine ELISA Kit standard curve
PDGFB,PDGFA elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS175775. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for PDGFB,PDGFA. It may not necessarily be applicable to this product.
 NCBI GI #    400744
 NCBI GeneID    18591
 NCBI Accession #    P31240.1 [Other Products]
Section Bar
 UniProt Primary Accession #    P31240 [Other Products]
 UniProt Secondary Accession #    Q6P3C4 [Other Products]
 UniProt Related Accession #    P31240 [Other Products]
 Molecular Weight    27,382 Da
Table BarTOPTable Bar
 NCBI Official Full Name    Platelet-derived growth factor subunit B
 NCBI Official Synonym Full Names    platelet derived growth factor, B polypeptide
 NCBI Official Symbol    Pdgfb [Similar Products]
 NCBI Official Synonym Symbols   
Sis; PDGF-B
[Similar Products]
 NCBI Protein Information    platelet-derived growth factor subunit B; c-sis; PDGF-2; PDGF subunit B; proto-oncogene c-Sis; platelet-derived growth factor B chain; platelet-derived growth factor beta polypeptide
 UniProt Protein Name    Platelet-derived growth factor subunit B
 UniProt Synonym Protein Names   
PDGF-2; Platelet-derived growth factor B chain; Platelet-derived growth factor beta polypeptide; Proto-oncogene c-Sis
 UniProt Gene Name    Pdgfb [Similar Products]
 UniProt Synonym Gene Names    Sis; PDGF subunit B [Similar Products]
 UniProt Entry Name    PDGFB_MOUSE
Section Bar
 UniProt Comments for PDGFB,PDGFA    PDGFB: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA. A chromosomal aberration involving PDGFB is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGFB. Belongs to the PDGF/VEGF growth factor family.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Oncoprotein; Secreted

Cellular Component: extracellular space; cell surface; membrane; basolateral plasma membrane; cytoplasm; extracellular region

Molecular Function: collagen binding; identical protein binding; protein homodimerization activity; growth factor activity; protein heterodimerization activity; platelet-derived growth factor binding; platelet-derived growth factor receptor binding; superoxide-generating NADPH oxidase activator activity; chemoattractant activity; receptor binding

Biological Process: embryonic placenta development; peptidyl-tyrosine phosphorylation; positive regulation of cyclin-dependent protein kinase activity; positive regulation of transcription, DNA-dependent; heart development; multicellular organismal development; positive regulation of smooth muscle cell proliferation; positive regulation of fibroblast growth factor receptor signaling pathway; platelet-derived growth factor receptor signaling pathway; cell projection biogenesis; positive regulation of smooth muscle cell migration; protein amino acid phosphorylation; monocyte chemotaxis; positive regulation of MAP kinase activity; positive regulation of fibroblast proliferation; negative regulation of vasodilation; positive chemotaxis; positive regulation of MAPKKK cascade; positive regulation of glomerular filtration; positive regulation of cell proliferation; response to wounding; positive regulation of mitotic cell cycle, embryonic; negative regulation of cell migration; regulation of peptidyl-tyrosine phosphorylation; substrate-bound cell migration; positive regulation of mitosis; positive regulation of chemotaxis; activation of protein kinase B; negative regulation of phosphatidylinositol biosynthetic process; positive regulation of blood vessel endothelial cell migration; positive regulation of phosphoinositide 3-kinase activity; positive regulation of protein amino acid autophosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of peptidyl-tyrosine phosphorylation; peptidyl-serine phosphorylation; positive regulation of cell division; positive regulation of vasoconstriction; activation of protein kinase activity; blood vessel morphogenesis; positive regulation of endothelial cell proliferation; negative regulation of transcription, DNA-dependent; blood coagulation; actin cytoskeleton organization and biogenesis; positive regulation of DNA replication; positive regulation of cell migration
Table BarTOPTable Bar
 Research Articles on PDGFB,PDGFA    1. These findings thus implicate a novel role of PDGF-BB in the migration of pericytes, resulting in loss of pericyte coverage from the endothelium with a subsequent breach of the blood-brain barrier
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Pathways associated with PDGFB,PDGFA elisa kitDiseases associated with PDGFB,PDGFA elisa kit
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Constitutive PI3K/AKT Signaling In Cancer Pathway antibodies  Constitutive PI3K/AKT Signaling In Cancer Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Downstream Signal Transduction Pathway antibodies  Downstream Signal Transduction Pathway Diagram
 Downstream Signaling Events Of B Cell Receptor (BCR) Pathway antibodies  Downstream Signaling Events Of B Cell Receptor (BCR) Pathway Diagram
 Downstream Signaling Of Activated FGFR Pathway antibodies  Downstream Signaling Of Activated FGFR Pathway Diagram
 Disease Name  Pubmed Publications
 Fibrosis Antibodies  >237 publications with PDGFB,PDGFA and Fibrosis
 Atherosclerosis Antibodies  >192 publications with PDGFB,PDGFA and Atherosclerosis
 Inflammation Antibodies  >143 publications with PDGFB,PDGFA and Inflammation
 Lung Diseases Antibodies  >129 publications with PDGFB,PDGFA and Lung Diseases
 Necrosis Antibodies  >127 publications with PDGFB,PDGFA and Necrosis
 Hyperplasia Antibodies  >115 publications with PDGFB,PDGFA and Hyperplasia
 Skin Neoplasms Antibodies  >110 publications with PDGFB,PDGFA and Skin Neoplasms
 Kidney Diseases Antibodies  >99 publications with PDGFB,PDGFA and Kidney Diseases
 Liver Diseases Antibodies  >99 publications with PDGFB,PDGFA and Liver Diseases
 Hypertension Antibodies  >96 publications with PDGFB,PDGFA and Hypertension
Organs/Tissues associated with PDGFB,PDGFA elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >668 publications with PDGFB,PDGFA and Blood
 Skin Antibodies  >275 publications with PDGFB,PDGFA and Skin
 Brain Antibodies  >211 publications with PDGFB,PDGFA and Brain
 Embryonic Tissue Antibodies  >209 publications with PDGFB,PDGFA and Embryonic Tissue
 Lung Antibodies  >199 publications with PDGFB,PDGFA and Lung
 Heart Antibodies  >138 publications with PDGFB,PDGFA and Heart
 Eye Antibodies  >103 publications with PDGFB,PDGFA and Eye
 Mixed Antibodies  >33 publications with PDGFB,PDGFA and Mixed
 Ovary Antibodies  >21 publications with PDGFB,PDGFA and Ovary
 Spleen Antibodies  >17 publications with PDGFB,PDGFA and Spleen
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions