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PIN4 elisa kit :: Mouse PIN4 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2515090
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
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 Product Name   


★Popular Item★
 Also Known As   

Mouse PIN4 (Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting 4 Protein) ELISA Kit

 Product Gene Name   

PIN4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    300252
 3D Structure    ModBase 3D Structure for Q9Y237
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 Species Reactivity    Mouse
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 Specificity    This kit recognizes natural and recombinant Mouse PIN4. No significant cross-reactivity or interference between Mouse PIN4 and analogues was observed.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    0.156-10ng/mL
 Sensitivity    Min: 0.094ng/mL; Max: 10ng/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PIN4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PIN4 purchase    MBS2515090 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the PIN4, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing PIN4, with an estimated sensitivity of Min: 0.094ng/mL; Max: 10ng/mL. The ELISA analytical biochemical technique of the MBS2515090 kit is based on PIN4 antibody-PIN4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PIN4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PIN4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PIN4 elisa kit

   Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse PIN4 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PIN4. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PIN4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PIN4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PIN4. You can calculate the concentration of PIN4 in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of PIN4 elisa kit    PIN4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2515090. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PIN4. It may not necessarily be applicable to this product.
 NCBI GI #    282847476
 NCBI GeneID    5303
 NCBI Accession #    NP_001164218.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001170747.1 [Other Products]
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 UniProt Primary Accession #    Q9Y237 [Other Products]
 UniProt Secondary Accession #    Q0D2H3; Q3MHV0; Q52M21; Q5HYW6; Q6IRW4; A8E0G6; B3KXM0; F5H1P5 [Other Products]
 UniProt Related Accession #    Q9Y237 [Other Products]
 Molecular Weight    14,179 Da
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 NCBI Official Full Name    peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 isoform 2
 NCBI Official Synonym Full Names    protein (peptidylprolyl cis/trans isomerase) NIMA-interacting, 4 (parvulin)
 NCBI Official Symbol    PIN4 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    peptidyl-prolyl cis-trans isomerase NIMA-interacting 4; PPIase PIN4; eukaryotic parvulin homolog; hEPVH; hPar14; hPar17; parvulin-14; parvulin-17; peptidyl-prolyl cis-trans isomerase Pin4; peptidyl-prolyl cis/trans isomerase EPVH; rotamase PIN4
 UniProt Protein Name    Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4
 UniProt Synonym Protein Names   
Parvulin-14; Par14; hPar14; Parvulin-17; Par17; hPar17; Peptidyl-prolyl cis-trans isomerase Pin4; PPIase Pin4; Peptidyl-prolyl cis/trans isomerase EPVH; hEPVH; Rotamase Pin4
 Protein Family    Peptidyl-prolyl cis-trans isomerase
 UniProt Gene Name    PIN4 [Similar Products]
 UniProt Synonym Gene Names    Par14; hPar14; Par17; hPar17; PPIase Pin4; hEPVH [Similar Products]
 UniProt Entry Name    PIN4_HUMAN
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 NCBI Summary for PIN4    This gene encodes a member of the parvulin subfamily of the peptidyl-prolyl cis/trans isomerase protein family. The encoded protein catalyzes the isomerization of peptidylprolyl bonds, and may play a role in the cell cycle, chromatin remodeling, and/or ribosome biogenesis. The encoded protein may play an additional role in the mitochondria. [provided by RefSeq, Dec 2009]
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 UniProt Comments for PIN4    PIN4: Isoform 1 is involved as a ribosomal RNA processing factor in ribosome biogenesis. Binds to tightly bent AT-rich stretches of double-stranded DNA. Belongs to the PpiC/parvulin rotamase family. PIN4 subfamily. 2 isoforms of the human protein are produced by alternative promoter.

Protein type: EC; Isomerase; Nucleolus; Mitochondrial

Chromosomal Location of Human Ortholog: Xq13

Cellular Component: mitochondrial matrix; cytoplasm; nucleolus; spindle; nucleus

Molecular Function: peptidyl-prolyl cis-trans isomerase activity; DNA binding; double-stranded DNA binding; bent DNA binding

Biological Process: protein peptidyl-prolyl isomerization; rRNA processing
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 Research Articles on PIN4    1. Data indicate the association of Par14 with insulin receptor substrate 1 (IRS-1) in human HepG2 cells overexpressing both as well as endogenously in the mouse liver.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with PIN4 elisa kitOrgans/Tissues associated with PIN4 elisa kit
 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >2 publications with PIN4 and Adenocarcinoma
 Cholestasis Antibodies  >1 publications with PIN4 and Cholestasis
 Precancerous Conditions Antibodies  >1 publications with PIN4 and Precancerous Conditions
 Hyperplasia Antibodies  >1 publications with PIN4 and Hyperplasia
 Liver Diseases Antibodies  >1 publications with PIN4 and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >3 publications with PIN4 and Embryonic Tissue
 Prostate Antibodies  >3 publications with PIN4 and Prostate
 Vascular Antibodies  >2 publications with PIN4 and Vascular
 Bladder Antibodies  >1 publications with PIN4 and Bladder
 Liver Antibodies  >1 publications with PIN4 and Liver
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