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Park2 elisa kit :: Mouse Parkinson disease 2/parkin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS723678
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Parkinson disease 2/parkin (Park2), ELISA Kit

 Also Known As   

Mouse Parkinson disease 2/parkin ELISA Kit

 Product Synonym Names    Mouse Parkinson disease 2/parkin ELISA Kit; Parkinson disease 2/parkin; Parkinson disease 2/parkin (Mouse)
 Product Gene Name   

Park2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 Chromosome Location    Chromosome: 6; NC_000006.11 (161768590..163148834, complement). Location: 6q25.2-q27
 OMIM    168600
 3D Structure    ModBase 3D Structure for O60260
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 Species Reactivity    Mouse
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 Specificity    Sensitivity: The sensitivity in this assay is 1.0 pg/mL.

Specificity: This assay has high sensitivity and excellent specificity for detection of PD2. No significant cross-reactivity or interference between PD2 and analogues was observed.
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 Samples    Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma
 Assay Type    Sandwich
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact technical support.
 Other Notes    Small volumes of Park2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Park2 purchase    MBS723678 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Parkinson disease 2/parkin (Park2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards render a theoretical kit detection range in biological research samples containing Park2. The ELISA analytical biochemical technique of the MBS723678 kit is based on Park2 antibody-Park2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Park2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Park2. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions such as Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for Park2 elisa kit

   For Samples: Cell culture fluid & body fluid & tissue homogenate serum or blood plasma

Intended Uses: This PD2 ELISA kit is intended for Laboratory research use only and is not for use in diagnostic or therapeutic procedures.The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of PD2 in the sample, this PD2 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus PD2 concentration. The concentration of PD2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: This PD2 enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a polyclonal antibody specific for PD2. Standards or samples are then added to the microtiter plate wells and PD2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PD2 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for PD2 are added to each well to "sandwich" the PD2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PD2 and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.
 Product Categories/Family for Park2 elisa kit    Mouse ELISA Kit
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 Typical Testing Data/Standard Curve (for reference only) of Park2 elisa kit    Park2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS723678
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NCBI/Uniprot data below describe general gene information for Park2. It may not necessarily be applicable to this product.
 NCBI GI #    18314633
 NCBI GeneID    5071
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 UniProt Primary Accession #    O60260 [Other Products]
 UniProt Secondary Accession #    Q5TFV8; Q6Q2I6; Q8NI41; Q8NI43; Q8NI44; Q8WW07; A3FG77; A8K975 [Other Products]
 UniProt Related Accession #    O60260; Q5VVX1; Q5VVX3; Q5VVX4; Q5XNR7; Q5XNR8; Q6Q2I7; Q6Q2I8; Q6S8G7; Q8NI42 [Other Products]
 Molecular Weight    51,641 Da
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 NCBI Official Full Name    PARK2 protein
 NCBI Official Synonym Full Names    parkinson protein 2, E3 ubiquitin protein ligase (parkin)
 NCBI Official Symbol    PARK2 [Similar Products]
 NCBI Official Synonym Symbols   
PDJ; PRKN; AR-JP; LPRS2
[Similar Products]
 NCBI Protein Information    E3 ubiquitin-protein ligase parkin; parkin 2; OTTHUMP00000017562; OTTHUMP00000017563; OTTHUMP00000017564; OTTHUMP00000017565; OTTHUMP00000017566; OTTHUMP00000017567; E3 ubiquitin ligase; parkinson disease protein 2; parkinson juvenile disease protein 2; Parkinson disease (autosomal recessive, juvenile) 2, parkin
 UniProt Protein Name    E3 ubiquitin-protein ligase parkin
 UniProt Synonym Protein Names   
Parkinson juvenile disease protein 2
 Protein Family    E3 ubiquitin-protein ligase
 UniProt Gene Name    PARK2 [Similar Products]
 UniProt Synonym Gene Names    PRKN [Similar Products]
 UniProt Entry Name    PRKN2_HUMAN
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 NCBI Summary for Park2    The precise function of this gene is unknown; however, the encoded protein is a component of a multiprotein E3 ubiquitin ligase complex that mediates the targeting of substrate proteins for proteasomal degradation. Mutations in this gene are known to cause Parkinson disease and autosomal recessive juvenile Parkinson disease. Alternative splicing of this gene produces multiple transcript variants encoding distinct isoforms. Additional splice variants of this gene have been described but currently lack transcript support. [provided by RefSeq]
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 UniProt Comments for Park2    Function: Functions within a multiprotein E3 ubiquitin ligase complex, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins, such as BCL2, SYT11, CCNE1, GPR37, STUB1, a 22 kDa O-linked glycosylated isoform of SNCAIP, SEPT5, ZNF746 and AIMP2. Mediates monoubiquitination as well as 'Lys-48'-linked and 'Lys-63'-linked polyubiquitination of substrates depending on the context. Participates in the removal and/or detoxification of abnormally folded or damaged protein by mediating 'Lys-63'-linked polyubiquitination of misfolded proteins such as PARK7: 'Lys-63'-linked polyubiquitinated misfolded proteins are then recognized by HDAC6, leading to their recruitment to aggresomes, followed by degradation. Mediates 'Lys-63'-linked polyubiquitination of SNCAIP, possibly playing a role in Lewy-body formation. Mediates monoubiquitination of BCL2, thereby acting as a positive regulator of autophagy. Promotes the autophagic degradation of dysfunctional depolarized mitochondria. Mediates 'Lys-48'-linked polyubiquitination of ZNF746, followed by degradation of ZNF746 by the proteasome; possibly playing a role in role in regulation of neuron death. Limits the production of reactive oxygen species (ROS). Loss of this ubiquitin ligase activity appears to be the mechanism underlying pathogenesis of PARK2. May protect neurons against alpha synuclein toxicity, proteasomal dysfunction, GPR37 accumulation, and kainate-induced excitotoxicity. May play a role in controlling neurotransmitter trafficking at the presynaptic terminal and in calcium-dependent exocytosis. Regulates cyclin-E during neuronal apoptosis. May represent a tumor suppressor gene. Ref.10 Ref.11 Ref.20 Ref.22 Ref.24 Ref.26 Ref.27 Ref.31 Ref.32 Ref.33 Ref.35 Ref.36 Ref.37 Ref.38

Pathway: Protein modification; protein ubiquitination.

Subunit structure: Forms an E3 ubiquitin ligase complex with UBE2L3 or UBE2L6. Mediates 'Lys-63'-linked polyubiquitination by associating with UBE2V1. Part of a SCF-like complex, consisting of PARK2, CUL1 and FBXW7. Interacts with SNCAIP. Binds to the C2A and C2B domains of SYT11. Interacts and regulates the turnover of SEPT5. Part of a complex, including STUB1, HSP70 and GPR37. The amount of STUB1 in the complex increases during ER stress. STUB1 promotes the dissociation of HSP70 from PARK2 and GPR37, thus facilitating PARK2-mediated GPR37 ubiquitination. HSP70 transiently associates with unfolded GPR37 and inhibits the E3 activity of PARK2, whereas, STUB1 enhances the E3 activity of PARK2 through promotion of dissociation of HSP70 from PARK2-GPR37 complexes. Interacts with PSMD4 and PACRG. Interacts with LRRK2. Interacts with RANBP2. Interacts with SUMO1 but not SUMO2, which promotes nuclear localization and autoubiquitination. Interacts (via first RING-type domain) with AIMP2 (via N-terminus). Interacts with PSMA7 and RNF41. Interacts with PINK1. Ref.12 Ref.14 Ref.17 Ref.18 Ref.19 Ref.20 Ref.25 Ref.26 Ref.27 Ref.28 Ref.29 Ref.30 Ref.33 Ref.34 Ref.35 Ref.36 Ref.37 Ref.38 Ref.39

Subcellular location: Cytoplasm › cytosol. Nucleus. Endoplasmic reticulum. Mitochondrion. Note: Mainly localizes in the cytosol. Co-localizes with SYT11 in neutrites. Co-localizes with SNCAIP in brainstem Lewy bodies. Relocates to dysfunctional mitochondria that have lost the mitochondial membrane potential; recruitement to mitochondria is PINK1-dependent. Ref.2 Ref.9 Ref.15 Ref.30 Ref.31 Ref.32 Ref.35 Ref.36

Tissue specificity: Highly expressed in the brain including the substantia nigra. Expressed in heart, testis and skeletal muscle. Expression is down-regulated or absent in tumor biopsies, and absent in the brain of PARK2 patients. Overexpression protects dopamine neurons from kainate-mediated apoptosis. Found in serum (at protein level). Ref.2

Domain: The ubiquitin-like domain binds the PSMD4 subunit of 26S proteasomes. Ref.16

Post-translational modification: Auto-ubiquitinates in an E2-dependent manner leading to its own degradation. Also polyubiquitinated by RNF41 for proteasomal degradation.S-nitrosylated. The inhibition of PARK2 ubiquitin E3 ligase activity by S-nitrosylation could contribute to the degenerative process in PD by impairing the ubiquitination of PARK2 substrates.

Involvement in disease: Defects in PARK2 are a cause of Parkinson disease (PARK) [

MIM:168600]. A complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability. Additional features are characteristic postural abnormalities, dysautonomia, dystonic cramps, and dementia. The pathology of Parkinson disease involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain. The disease is progressive and usually manifests after the age of 50 years, although early-onset cases (before 50 years) are known. The majority of the cases are sporadic suggesting a multifactorial etiology based on environmental and genetic factors. However, some patients present with a positive family history for the disease. Familial forms of the disease usually begin at earlier ages and are associated with atypical clinical features. Ref.1 Ref.11 Ref.14 Ref.15 Ref.18 Ref.35 Ref.36 Ref.37 Ref.40 Ref.42 Ref.44 Ref.46 Ref.48 Ref.49 Ref.50 Ref.51 Ref.52 Ref.54 Ref.55 Ref.56 Ref.57 Ref.58 Ref.59 Ref.60 Ref.62Defects in PARK2 are the cause of Parkinson disease type 2 (PARK2) [

MIM:600116]; also known as early-onset parkinsonism with diurnal fluctuation (EPDF) or autosomal recessive juvenile Parkinson disease (PDJ). A neurodegenerative disorder characterized by bradykinesia, rigidity, postural instability, tremor, and onset usually befor 40. It differs from classic Parkinson disease by early DOPA-induced dyskinesia, diurnal fluctuation of the symptoms, sleep benefit, dystonia and hyper-reflexia. Dementia is absent. Pathologically, patients show loss of dopaminergic neurons in the substantia nigra, similar to that seen in Parkinson disease; however, Lewy bodies (intraneuronal accumulations of aggregated proteins) are absent. Ref.1 Ref.14 Ref.35 Ref.40 Ref.42 Ref.44 Ref.46 Ref.50 Ref.51 Ref.54Note=Defects in PARK2 may be involved in the development and/or progression of ovarian cancer.

Miscellaneous: The parkin locus (PRKN), adjacent to the 6q telomere is hyper-recombinable and lies within FRA6E, the third most common fragile site in tumor tissue.Members of the RBR family are atypical E3 ligases. They interact with the E2 conjugating enzyme UBE2L3 and function like HECT-type E3 enzymes: they bind E2s via the first RING domain, but require an obligate trans-thiolation step during the ubiquitin transfer, requiring a conserved cysteine residue in the second RING domain (Ref.38).

Sequence similarities: Belongs to the RBR family. Parkin subfamily.Contains 1 IBR-type zinc finger.Contains 2 RING-type zinc fingers.Contains 1 ubiquitin-like domain.
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 Research Articles on Park2    1. The results of this study indicated that genetic variants at the parkin and PINK1 loci do not play a critical role in the pathogenesis of multiple system atrophy.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products related to Mouse Parkinson disease 2/parkin ELISA Kit
 Catalog #  Product Name  Catalog #  Product Name
 MBS729126  Guinea Pig Parkinson disease 2/parkin ELISA Kit  MBS723678  Mouse Parkinson disease 2/parkin ELISA Kit
 MBS732560  Rabbit Parkinson disease 2/parkin ELISA Kit  MBS722554  Rat Parkinson disease 2/parkin ELISA Kit
 MBS732278  Human Parkinson disease 2/parkin ELISA Kit
Products associated with Park2 elisa kitPathways associated with Park2 elisa kit
 Reference Product  PubMed Publications
 SNCAIP elisa kit  >6 publications with Park2 and SNCAIP
 UBE2L3 elisa kit  >3 publications with Park2 and UBE2L3
 GPR37 elisa kit  >1 publications with Park2 and GPR37
 AIMP2 elisa kit  >1 publications with Park2 and AIMP2
 Products by Pathway  Pathway Diagram
 Adaptive Immunity Signaling Pathway antibodies  Adaptive Immunity Signaling Pathway Diagram
 Alpha-synuclein Signaling Pathway antibodies  Alpha-synuclein Signaling Pathway Diagram
 Antigen Processing: Ubiquitination & Proteasome Degradation Pathway antibodies  Antigen Processing: Ubiquitination & Proteasome Degradation Pathway Diagram
 Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies  Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Parkinson's Disease Pathway antibodies  Parkinson's Disease Pathway Diagram
 Protein Processing In Endoplasmic Reticulum Pathway antibodies  Protein Processing In Endoplasmic Reticulum Pathway Diagram
 Protein Processing In Endoplasmic Reticulum Pathway antibodies  Protein Processing In Endoplasmic Reticulum Pathway Diagram
 Ubiquitin Mediated Proteolysis Pathway antibodies  Ubiquitin Mediated Proteolysis Pathway Diagram
 Ubiquitin Mediated Proteolysis Pathway antibodies  Ubiquitin Mediated Proteolysis Pathway Diagram
Diseases associated with Park2 elisa kitOrgans/Tissues associated with Park2 elisa kit
 Disease Name  Pubmed Publications
 Brain Diseases Antibodies  >122 publications with Park2 and Brain Diseases
 Parkinsonian Disorders Antibodies  >115 publications with Park2 and Parkinsonian Disorders
 Parkinson Disease Antibodies  >103 publications with Park2 and Parkinson Disease
 Neurodegenerative Diseases Antibodies  >93 publications with Park2 and Neurodegenerative Diseases
 Leprosy Antibodies  >12 publications with Park2 and Leprosy
 Nerve Degeneration Antibodies  >9 publications with Park2 and Nerve Degeneration
 Alzheimer Disease Antibodies  >6 publications with Park2 and Alzheimer Disease
 Neoplasms, Experimental Antibodies  >5 publications with Park2 and Neoplasms, Experimental
 Colorectal Neoplasms Antibodies  >3 publications with Park2 and Colorectal Neoplasms
 Adenocarcinoma Antibodies  >3 publications with Park2 and Adenocarcinoma
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >49 publications with Park2 and Brain
 Kidney Antibodies  >3 publications with Park2 and Kidney
 Eye Antibodies  >2 publications with Park2 and Eye
 Stomach Antibodies  >1 publications with Park2 and Stomach
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