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PYY elisa kit :: Mouse Peptide YY ELISA Kit

Scan QR to view Datasheet Catalog #    MBS725360
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Peptide YY (PYY), ELISA Kit

 Also Known As   

Mouse Peptide YY ELISA Kit

 Product Gene Name   

PYY elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    600781
 3D Structure    ModBase 3D Structure for P10082
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 Species Reactivity    Mouse
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    1.0 pg/mL.
 Intended Uses    This PYY ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of mouse PYY. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PYY elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PYY purchase    MBS725360 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Peptide YY (PYY) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PYY, with an estimated sensitivity of 1.0 pg/mL. The ELISA analytical biochemical technique of the MBS725360 kit is based on PYY antibody-PYY antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PYY antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PYY. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for PYY elisa kit

   Principle of the Assay: PYY ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PYY antibody and an PYY-HRP conjugate. The assay sample and buffer are incubated together with PYY-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PYY concentration since PYY from samples and PYY-HRP conjugate compete for the anti-PYY antibody binding site. Since the number of sites is limited, as more sites are occupied by PYY from the sample, fewer sites are left to bind PYY-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PYY concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for PYY elisa kit    Neurobiology
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 Typical Testing Data/Standard Curve (for reference only) of PYY elisa kit    PYY elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS725360. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PYY. It may not necessarily be applicable to this product.
 NCBI GI #    49457254
 NCBI GeneID    5697
 NCBI Accession #    CAG46926.1 [Other Products]
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 UniProt Primary Accession #    P10082 [Other Products]
 UniProt Secondary Accession #    Q5U5Q6; Q6FGH8 [Other Products]
 UniProt Related Accession #    P10082 [Other Products]
 Molecular Weight    10,360 Da
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 NCBI Official Full Name    PYY
 NCBI Official Synonym Full Names    peptide YY
 NCBI Official Symbol    PYY [Similar Products]
 NCBI Official Synonym Symbols   
PYY1; PYY-I
[Similar Products]
 NCBI Protein Information    peptide YY; prepro-PYY; peptide tyrosine tyrosine
 UniProt Protein Name    Peptide YY
 UniProt Synonym Protein Names   
PYY-I; Peptide tyrosine tyrosineCleaved into the following chain:Peptide YY(3-36)Alternative name(s):PYY-II
 Protein Family    Peptide
 UniProt Gene Name    PYY [Similar Products]
 UniProt Synonym Gene Names    PYY [Similar Products]
 UniProt Entry Name    PYY_HUMAN
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 NCBI Summary for PYY    The protein encoded by this gene is proteolytically processed to release a peptide that inhibits pancreatic secretion and mobility in the gut. Rare variations in this gene could increase susceptibility to obesity. [provided by RefSeq, Jul 2010]
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 UniProt Comments for PYY    PYY: This gut peptide inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility. Belongs to the NPY family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 17q21.1

Cellular Component: extracellular space; extracellular region

Molecular Function: neuropeptide hormone activity; protein binding; G-protein-coupled receptor binding; hormone activity

Biological Process: G-protein coupled receptor protein signaling pathway; cell proliferation; cell-cell signaling; neuropeptide signaling pathway; eating behavior; regulation of appetite; digestion; feeding behavior; cytoskeleton organization and biogenesis; cell motility

Disease: Obesity
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 Research Articles on PYY    1. PYY and ghrelin are reciprocally associated during a period of weight stability, but not following weight loss
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PYY elisa kitPathways associated with PYY elisa kit
 Reference Product  PubMed Publications
 CCK elisa kit  >229 publications with PYY and CCK
 POMC elisa kit  >45 publications with PYY and POMC
 SST elisa kit  >7 publications with PYY and SST
 NPY2R elisa kit  >6 publications with PYY and NPY2R
 GAL elisa kit  >3 publications with PYY and GAL
 NPY1R elisa kit  >3 publications with PYY and NPY1R
 NPY5R elisa kit  >1 publications with PYY and NPY5R
 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Peptide Ligand-binding Receptors Pathway antibodies  Peptide Ligand-binding Receptors Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By GPCR Pathway antibodies  Signaling By GPCR Pathway Diagram
Diseases associated with PYY elisa kitOrgans/Tissues associated with PYY elisa kit
 Disease Name  Pubmed Publications
 Obesity Antibodies  >393 publications with PYY and Obesity
 Weight Loss Antibodies  >163 publications with PYY and Weight Loss
 Gastrointestinal Diseases Antibodies  >124 publications with PYY and Gastrointestinal Diseases
 Weight Gain Antibodies  >87 publications with PYY and Weight Gain
 Insulin Resistance Antibodies  >73 publications with PYY and Insulin Resistance
 Diabetes Mellitus, Type 2 Antibodies  >59 publications with PYY and Diabetes Mellitus, Type 2
 Anorexia Antibodies  >57 publications with PYY and Anorexia
 Hyperinsulinism Antibodies  >46 publications with PYY and Hyperinsulinism
 Carcinoma Antibodies  >45 publications with PYY and Carcinoma
 Inflammation Antibodies  >42 publications with PYY and Inflammation
 Organ/Tissue Name  Pubmed Publications
 Intestine Antibodies  >492 publications with PYY and Intestine
 Brain Antibodies  >395 publications with PYY and Brain
 Kidney Antibodies  >63 publications with PYY and Kidney
 Testis Antibodies  >2 publications with PYY and Testis
 Uncharacterized Tissue Antibodies  >1 publications with PYY and Uncharacterized Tissue
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