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PPAR-beta elisa kit :: Mouse Peroxisome Proliferator-activated Receptor beta (PPAR-beta) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS903532
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Peroxisome Proliferator-activated Receptor beta (PPAR-beta), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Peroxisome Proliferator-activated Receptor beta, PPAR-beta ELISA Kit

 Product Synonym Names    Mouse Peroxisome Proliferator-activated Receptor beta (PPAR-beta) ELISA Kit; FAAR; MGC3931; NR1C2; NUC1; NUCI; NUCII; PPAR-beta; PPARB; OTTHUMP00000016256; OTTHUMP00000016257; nuclear hormone receptor 1; peroxisome proliferative activated receptor; delta; Peroxisome Proliferator-activated Receptor beta (PPAR-beta)
 Product Gene Name   

PPAR-beta elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS903532 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P35396
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse PPAR-beta. No significant cross-reactivity or interference between mouse PPAR-beta and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, cell culture supernates
 Assay Type    Sandwich
 Detection Range    0.312 ng/ml-20 ng/ml.
 Sensitivity    The minimum detectable dose of mouse PPAR-beta is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PPAR-beta elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PPAR-beta purchase    MBS903532 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Peroxisome Proliferator-activated Receptor beta (PPAR-beta) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312 ng/ml-20 ng/ml in biological research samples containing PPAR-beta, with an estimated sensitivity of The minimum detectable dose of mouse PPAR-beta is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS903532 kit is based on PPAR-beta antibody-PPAR-beta antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PPAR-beta antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PPAR-beta. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell culture supernates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for PPAR-beta elisa kit

   Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PPAR-beta has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PPAR-beta present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPAR-beta is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPAR-beta bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of PPAR-beta elisa kit    PPAR-beta elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS903532. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PPAR-beta. It may not necessarily be applicable to this product.
 NCBI GI #    33859590
 NCBI GeneID    19015
 NCBI Accession #    NP_035275.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_011145.3 [Other Products]
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 UniProt Primary Accession #    P35396 [Other Products]
 UniProt Secondary Accession #    P37239 [Other Products]
 UniProt Related Accession #    P35396 [Other Products]
 Molecular Weight    49,715 Da
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 NCBI Official Full Name    peroxisome proliferator-activated receptor delta
 NCBI Official Synonym Full Names    peroxisome proliferator activator receptor delta
 NCBI Official Symbol    Ppard [Similar Products]
 NCBI Official Synonym Symbols   
NUC1; NUC-1; Nr1c2; Pparb; PPAR[b]; Pparb/d; PPAR-beta; PPARdelta; PPAR-delta
[Similar Products]
 NCBI Protein Information    peroxisome proliferator-activated receptor delta; PPARdelta/beta; nuclear hormone receptor 1; nuclear receptor subfamily 1 group C member 2; Peroxisome proliferator-activated receptor beta
 UniProt Protein Name    Peroxisome proliferator-activated receptor delta
 UniProt Synonym Protein Names   
Nuclear hormone receptor 1; NUC1; Nuclear receptor subfamily 1 group C member 2; Peroxisome proliferator-activated receptor beta; PPAR-beta
 UniProt Gene Name    Ppard [Similar Products]
 UniProt Synonym Gene Names    Nr1c2; Pparb; PPAR-delta; NUC1; PPAR-beta [Similar Products]
 UniProt Entry Name    PPARD_MOUSE
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 UniProt Comments for PPAR-beta    PPAR-delta: Ligand-activated transcription factor. Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand. Belongs to the nuclear hormone receptor family. NR1 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor; DNA-binding

Cellular Component: nucleus

Molecular Function: ligand-dependent nuclear receptor activity; zinc ion binding; prostacyclin receptor activity; metal ion binding; transcription coactivator activity; enzyme activator activity; drug binding; retinoid X receptor binding; NF-kappaB binding; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; steroid hormone receptor activity; fatty acid binding; lipid binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; positive regulation of catalytic activity; proteoglycan metabolic process; epidermis development; wound healing; negative regulation of smooth muscle cell proliferation; regulation of fat cell differentiation; positive regulation of transcription, DNA-dependent; negative regulation of collagen biosynthetic process; positive regulation of epidermis development; negative regulation of smooth muscle cell migration; negative regulation of transcription from RNA polymerase II promoter; positive regulation of vasodilation; vitamin A metabolic process; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; axon ensheathment; cell differentiation; negative regulation of epithelial cell proliferation; placenta development; intracellular receptor-mediated signaling pathway; transcription, DNA-dependent; positive regulation of insulin secretion; keratinocyte proliferation; keratinocyte migration; cell-substrate adhesion; regulation of cell proliferation; phospholipid biosynthetic process; positive regulation of phosphoinositide 3-kinase cascade; regulation of transcription from RNA polymerase II promoter; cell proliferation; G-protein coupled receptor protein signaling pathway; negative regulation of inflammatory response; mRNA transcription; fatty acid oxidation; steroid hormone mediated signaling; negative regulation of cell growth; lipid metabolic process; anagen; cellular process; regulation of insulin secretion; embryo implantation; negative regulation of apoptosis
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 Research Articles on PPAR-beta    1. Data indicate that the fatty acid-binding protein 5 (FABP5)/nuclear receptor peroxisome proliferator-activated receptor beta/delta (PPARbeta/delta) pathway induces the expression of cognition-associated genes.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with PPAR-beta elisa kitDiseases associated with PPAR-beta elisa kit
 Products by Pathway  Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 Nuclear Receptor Transcription Pathway antibodies  Nuclear Receptor Transcription Pathway Diagram
 Nuclear Receptors Pathway antibodies  Nuclear Receptors Pathway Diagram
 Nuclear Receptors In Lipid Metabolism And Toxicity Pathway antibodies  Nuclear Receptors In Lipid Metabolism And Toxicity Pathway Diagram
 PPAR (Peroxisome Proliferator-activated Receptor) Signaling Pathway antibodies  PPAR (Peroxisome Proliferator-activated Receptor) Signaling Pathway Diagram
 PPAR Signaling Pathway antibodies  PPAR Signaling Pathway Diagram
 Disease Name  Pubmed Publications
 Obesity Antibodies  >15 publications with PPAR-beta and Obesity
 Insulin Resistance Antibodies  >13 publications with PPAR-beta and Insulin Resistance
 Cardiovascular Diseases Antibodies  >11 publications with PPAR-beta and Cardiovascular Diseases
 Diabetes Mellitus, Type 2 Antibodies  >8 publications with PPAR-beta and Diabetes Mellitus, Type 2
 Atherosclerosis Antibodies  >5 publications with PPAR-beta and Atherosclerosis
 Gastrointestinal Diseases Antibodies  >5 publications with PPAR-beta and Gastrointestinal Diseases
 Heart Diseases Antibodies  >5 publications with PPAR-beta and Heart Diseases
 Hyperlipidemias Antibodies  >4 publications with PPAR-beta and Hyperlipidemias
 Fatty Liver Antibodies  >4 publications with PPAR-beta and Fatty Liver
 Inflammation Antibodies  >4 publications with PPAR-beta and Inflammation
Organs/Tissues associated with PPAR-beta elisa kit
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >11 publications with PPAR-beta and Liver
 Heart Antibodies  >5 publications with PPAR-beta and Heart
 Embryonic Tissue Antibodies  >3 publications with PPAR-beta and Embryonic Tissue
 Intestine Antibodies  >3 publications with PPAR-beta and Intestine
 Brain Antibodies  >3 publications with PPAR-beta and Brain
 Skin Antibodies  >2 publications with PPAR-beta and Skin
 Thymus Antibodies  >1 publications with PPAR-beta and Thymus
 Spleen Antibodies  >1 publications with PPAR-beta and Spleen
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