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PEBP1 elisa kit :: Mouse Phosphatidylethanolamine Binding Protein 1 (PEBP1) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2000261 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Standard Curve/Testing Data
Unit / Price
24-Strip-Wells  /  $270 +1 FREE 8GB USB
48-Strip-Wells  /  $505 +1 FREE 8GB USB
96-Strip-Wells  /  $685 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,770 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,025 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Phosphatidylethanolamine Binding Protein 1 (PEBP1), ELISA Kit

★Popular Item★
 Also Known As   

Phosphatidylethanolamine Binding Protein 1 (PEBP1) ELISA Kit

 Product Synonym Names    PBP; HCNP; PEBP; RKIP; Prostatic Binding Protein; Raf Kinase Inhibitory Protein; Hippocampal Cholinergic Neurostimulating Peptide; Neuropolypeptide h3
 Product Gene Name   

PEBP1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    604591
 3D Structure    ModBase 3D Structure for P30086
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Phosphatidylethanolamine Binding Protein 1 (PEBP1).
No significant cross-reactivity or interference between Phosphatidylethanolamine Binding Protein 1 (PEBP1) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Tissue homogenates, Cell lysates and Other Biological Fluids
 Detection Range    0.156-10ng/mL
 Sensitivity    < 0.054ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Phosphatidylethanolamine Binding Protein 1 (PEBP1) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Phosphatidylethanolamine Binding Protein 1 (PEBP1) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PEBP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PEBP1 purchase    MBS2000261 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Phosphatidylethanolamine Binding Protein 1 (PEBP1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing PEBP1, with an estimated sensitivity of < 0.054ng/mL. The ELISA analytical biochemical technique of the MBS2000261 kit is based on PEBP1 antibody-PEBP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PEBP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PEBP1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates, Cell lysates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PEBP1 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphatidylethanolamine Binding Protein 1 (PEBP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphatidylethanolamine Binding Protein 1 (PEBP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphatidylethanolamine Binding Protein 1 (PEBP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphatidylethanolamine Binding Protein 1 (PEBP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for PEBP1 elisa kit    Metabolic pathway
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 Typical Standard Curve/Testing Data of PEBP1 elisa kit    PEBP1 elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS2000261. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PEBP1. It may not necessarily be applicable to this product.
 NCBI GI #    21410340
 NCBI GeneID    5037
 NCBI Accession #    AAH31102.1 [Other Products]
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 UniProt Primary Accession #    P30086 [Other Products]
 UniProt Secondary Accession #    B2R4S1 [Other Products]
 UniProt Related Accession #    P30086 [Other Products]
 Molecular Weight    21,057 Da
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 NCBI Official Full Name    Phosphatidylethanolamine binding protein 1
 NCBI Official Synonym Full Names    phosphatidylethanolamine binding protein 1
 NCBI Official Symbol    PEBP1 [Similar Products]
 NCBI Official Synonym Symbols   
PBP; HCNP; PEBP; RKIP; HCNPpp; PEBP-1; HEL-210; HEL-S-34
[Similar Products]
 NCBI Protein Information    phosphatidylethanolamine-binding protein 1
 UniProt Protein Name    Phosphatidylethanolamine-binding protein 1
 UniProt Synonym Protein Names   
HCNPpp; Neuropolypeptide h3; Prostatic-binding protein; Raf kinase inhibitor protein; RKIP
 Protein Family    Phosphatidylethanolamine-binding protein
 UniProt Gene Name    PEBP1 [Similar Products]
 UniProt Synonym Gene Names    PBP; PEBP; PEBP-1; RKIP; HCNP [Similar Products]
 UniProt Entry Name    PEBP1_HUMAN
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 NCBI Summary for PEBP1    This gene encodes a member of the phosphatidylethanolamine-binding family of proteins and has been shown to modulate multiple signaling pathways, including the MAP kinase (MAPK), NF-kappa B, and glycogen synthase kinase-3 (GSK-3) signaling pathways. The encoded protein can be further processed to form a smaller cleavage product, hippocampal cholinergic neurostimulating peptide (HCNP), which may be involved in neural development. This gene has been implicated in numerous human cancers and may act as a metastasis suppressor gene. Multiple pseudogenes of this gene have been identified in the genome. [provided by RefSeq, Jul 2015]
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 UniProt Comments for PEBP1    RKIP: a metastasis suppressor that regulates extracellular matrix remodeling and tumor survival. Binds to and inhibits Raf-1. Phosphorylation by classic and atypical but not novel PKC isoforms dissociates this complex, relieving inhibition of the Raf/MAP kinase signaling cascade. Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase Inhibits the kinase activity of RAF1 by inhibiting its activation and by dissociating the RAF1/MEK complex and acting as a competitive inhibitor of MEK phosphorylation. Cleaved into hippocampal cholinergic neurostimulating peptide (HCMP) that appears to be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Expressed in brain. Increased expression in aged senescence-accelerated mice.

Protein type: Protein kinase, regulatory subunit; Lipid-binding

Chromosomal Location of Human Ortholog: 12q24.23

Cellular Component: cytoplasm; nucleus

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; enzyme binding; phosphatidylethanolamine binding; protein kinase binding; ATP binding
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 Research Articles on PEBP1    1. downregulation of RKIP occurs frequently... suggesting RKIP being a critical tumor suppressor for clear cell renal cell carcinomas
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PEBP1 elisa kitPathways associated with PEBP1 elisa kit
 Reference Product  PubMed Publications
 ADRBK1 elisa kit  >6 publications with PEBP1 and ADRBK1
 MAPK1 elisa kit  >4 publications with PEBP1 and MAPK1
 AURKB elisa kit  >1 publications with PEBP1 and AURKB
 RAF1 elisa kit  >1 publications with PEBP1 and RAF1
 Products by Pathway  Pathway Diagram
 Aurora B Signaling Pathway antibodies  Aurora B Signaling Pathway Diagram
 EGFR1 Signaling Pathway antibodies  EGFR1 Signaling Pathway Diagram
 ErbB1 Downstream Signaling Pathway antibodies  ErbB1 Downstream Signaling Pathway Diagram
 TNF-alpha/NF-kB Signaling Pathway antibodies  TNF-alpha/NF-kB Signaling Pathway Diagram
Diseases associated with PEBP1 elisa kitOrgans/Tissues associated with PEBP1 elisa kit
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >53 publications with PEBP1 and Carcinoma
 Disease Models, Animal Antibodies  >14 publications with PEBP1 and Disease Models, Animal
 Liver Diseases Antibodies  >10 publications with PEBP1 and Liver Diseases
 Liver Neoplasms Antibodies  >9 publications with PEBP1 and Liver Neoplasms
 Nervous System Diseases Antibodies  >9 publications with PEBP1 and Nervous System Diseases
 Brain Diseases Antibodies  >9 publications with PEBP1 and Brain Diseases
 Necrosis Antibodies  >8 publications with PEBP1 and Necrosis
 Lung Neoplasms Antibodies  >7 publications with PEBP1 and Lung Neoplasms
 Carcinoma, Hepatocellular Antibodies  >7 publications with PEBP1 and Carcinoma, Hepatocellular
 Cardiovascular Diseases Antibodies  >6 publications with PEBP1 and Cardiovascular Diseases
 Organ/Tissue Name  Pubmed Publications
 Prostate Antibodies  >43 publications with PEBP1 and Prostate
 Liver Antibodies  >30 publications with PEBP1 and Liver
 Blood Antibodies  >29 publications with PEBP1 and Blood
 Brain Antibodies  >28 publications with PEBP1 and Brain
 Vascular Antibodies  >16 publications with PEBP1 and Vascular
 Lymph Node Antibodies  >14 publications with PEBP1 and Lymph Node
 Lymph Antibodies  >14 publications with PEBP1 and Lymph
 Lung Antibodies  >11 publications with PEBP1 and Lung
 Stomach Antibodies  >11 publications with PEBP1 and Stomach
 Muscle Antibodies  >11 publications with PEBP1 and Muscle
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