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PTN elisa kit :: Mouse Pleiotrophin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS730540
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Pleiotrophin (PTN), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Pleiotrophin ELISA Kit

 Product Gene Name   

PTN elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    162095
 3D Structure    ModBase 3D Structure for P21246
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of PTN. No significant cross-reactivity or interference between PTN and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PTN and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    25-500pg/mL
 Sensitivity    1.0ng/mL
 Intended Uses    This PTN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse PTN. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PTN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PTN purchase    MBS730540 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Pleiotrophin (PTN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 25-500pg/mL in biological research samples containing PTN, with an estimated sensitivity of 1.0ng/mL. The ELISA analytical biochemical technique of the MBS730540 kit is based on PTN antibody-PTN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PTN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PTN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for PTN elisa kit

   Principle of the assay: PTN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PTN antibody and an PTN-HRP conjugate. The assay sample and buffer are incubated together with PTN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PTN concentration since PTN from samples and PTN-HRP conjugate compete for the anti-PTN antibody binding site. Since the number of sites is limited, as more sites are occupied by PTN from the sample, fewer sites are left to bind PTN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PTN concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for PTN elisa kit    Neurobiology
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 Typical Testing Data/Standard Curve (for reference only) of PTN elisa kit    PTN elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS730540. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PTN. It may not necessarily be applicable to this product.
 NCBI GI #    47496623
 NCBI Accession #    CAG29334.1 [Other Products]
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 UniProt Primary Accession #    P21246 [Other Products]
 UniProt Secondary Accession #    Q5U0B0; Q6ICQ5; Q9UCC6 [Other Products]
 Molecular Weight    18,942 Da
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 NCBI Official Full Name    PTN, partial
 UniProt Protein Name    Pleiotrophin
 UniProt Synonym Protein Names   
Heparin-binding brain mitogen; HBBM; Heparin-binding growth factor 8; HBGF-8; Heparin-binding growth-associated molecule; HB-GAM; Heparin-binding neurite outgrowth-promoting factor 1; HBNF-1; Osteoblast-specific factor 1; OSF-1
 Protein Family    Pleiotrophin
 UniProt Gene Name    PTN [Similar Products]
 UniProt Synonym Gene Names    HBNF1; NEGF1; PTN; HBBM; HBGF-8; HB-GAM; HBNF-1; OSF-1 [Similar Products]
 UniProt Entry Name    PTN_HUMAN
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 UniProt Comments for PTN    PTN: Secreted growth factor that induces neurite outgrowth and which is mitogenic for fibroblasts, epithelial, and endothelial cells. Binds anaplastic lymphoma kinase (ALK) which induces MAPK pathway activation, an important step in the anti- apoptotic signaling of PTN and regulation of cell proliferation. Belongs to the pleiotrophin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7q33

Cellular Component: extracellular space; endoplasmic reticulum

Molecular Function: heparin binding; growth factor activity; protein phosphatase inhibitor activity

Biological Process: nervous system development; positive regulation of cell division; positive regulation of cell proliferation; negative regulation of catalytic activity; learning; transmembrane receptor protein tyrosine phosphatase signaling pathway; bone mineralization
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PTN elisa kitDiseases associated with PTN elisa kit
 Reference Product  PubMed Publications
 ALK elisa kit  >24 publications with PTN and ALK
 PTPRZ1 elisa kit  >18 publications with PTN and PTPRZ1
 VEGFA elisa kit  >9 publications with PTN and VEGFA
 SDC3 elisa kit  >7 publications with PTN and SDC3
 SRC elisa kit  >4 publications with PTN and SRC
 PTEN elisa kit  >3 publications with PTN and PTEN
 SDC1 elisa kit  >3 publications with PTN and SDC1
 KDR elisa kit  >2 publications with PTN and KDR
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >63 publications with PTN and Carcinoma
 Pain Antibodies  >33 publications with PTN and Pain
 Disease Models, Animal Antibodies  >24 publications with PTN and Disease Models, Animal
 Lung Neoplasms Antibodies  >21 publications with PTN and Lung Neoplasms
 Inflammation Antibodies  >16 publications with PTN and Inflammation
 Necrosis Antibodies  >15 publications with PTN and Necrosis
 Heart Diseases Antibodies  >14 publications with PTN and Heart Diseases
 Neoplasms, Experimental Antibodies  >14 publications with PTN and Neoplasms, Experimental
 Carcinoma, Squamous Cell Antibodies  >12 publications with PTN and Carcinoma, Squamous Cell
 Kidney Diseases Antibodies  >9 publications with PTN and Kidney Diseases
Organs/Tissues associated with PTN elisa kit
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >188 publications with PTN and Brain
 Bone Antibodies  >71 publications with PTN and Bone
 Vascular Antibodies  >66 publications with PTN and Vascular
 Muscle Antibodies  >58 publications with PTN and Muscle
 Embryonic Tissue Antibodies  >35 publications with PTN and Embryonic Tissue
 Lung Antibodies  >24 publications with PTN and Lung
 Heart Antibodies  >23 publications with PTN and Heart
 Kidney Antibodies  >19 publications with PTN and Kidney
 Skin Antibodies  >19 publications with PTN and Skin
 Connective Tissue Antibodies  >19 publications with PTN and Connective Tissue
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