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SPA elisa kit :: Mouse Pulmonary Surfactant Associated Protein A ELISA Kit

Scan QR to view Datasheet Catalog #    MBS022557 SPA elisa kit
Unit / Price
48-Strip-Wells  /  $435 +1 FREE 8GB USB
96-Strip-Wells  /  $600 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,650 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,205 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Pulmonary Surfactant Associated Protein A (SPA), ELISA Kit

 Also Known As   

Mouse Pulmonary Surfactant Associated Protein A ELISA Kit

 Product Gene Name   

SPA elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 Chromosome Location    Chromosome: 14; NC_010456.4 (88698851..88702099, complement). Location: 14; 14
 3D Structure    ModBase 3D Structure for P49874
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 Species Reactivity    Mouse
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 Specificity    No significant cross-reactivity or interference between this analyte and analogues is observed.
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 Assay Type    Quantitative Sandwich
 Detection Range    6.25 pg/ml - 200 pg/ml
 Sensitivity    1.0 pg/ml
 Intra-assay Precision    Intra-assay CV (%) is less than 15%.
 Inter-assay Precision    Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean 100].
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of SPA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for SPA purchase    MBS022557 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Pulmonary Surfactant Associated Protein A (SPA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 6.25 pg/ml - 200 pg/ml in biological research samples containing SPA, with an estimated sensitivity of 1.0 pg/ml. The ELISA analytical biochemical technique of the MBS022557 kit is based on SPA antibody-SPA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SPA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SPA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Mutation of pulmonary surfactant protein A SPA are a leading cause of respiratory problems. Surfactant protein SPA is produced by human lung alveolar cells and is a component of a highly complex mix of lipids and lectins which reduce membrane surface tension and protect lung function during exhalation. To study this effect in depth, we offer a specfic sandwich SPA 1 ELISA kit from ELabScience. Manufactured to CLIA kits standards, you know you can trust this assay precision and results. Shipped at room temperature but store at 4oC. These ELISA kits are self contained, with polyclonal antibody plate, detection reagent, standard curve peptide, HRP conjugate secondary antibodies, wash solution, sample buffer and stop solution. Like all our top quality reagents, your samples of cell proteins will be assayed right for detection surfactant factors. Besides surfactant protein ELISA, our catalog ranges from stem cells, molecular biology reagents to purinergic receptor p2x.
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Product Description specifically for SPA elisa kit

   Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of SPA (hereafter termed "analyte") in undiluted original Mouse body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
Sample Manual Insert of MBS022557
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NCBI/Uniprot data below describe general gene information for SPA. It may not necessarily be applicable to this product.
 NCBI GI #    47523604
 NCBI GeneID    397503
 NCBI Accession #    NP_999430.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_214265.1 [Other Products]
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 UniProt Primary Accession #    P49874 [Other Products]
 UniProt Related Accession #    P49874 [Other Products]
 Molecular Weight    26,702 Da
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 NCBI Official Full Name    pulmonary surfactant-associated protein A
 NCBI Official Symbol    SFTPA1 [Similar Products]
 NCBI Official Synonym Symbols   
PSP-A; SFTPA
[Similar Products]
 NCBI Protein Information    pulmonary surfactant-associated protein A; PSAP; SP-A
 UniProt Protein Name    Pulmonary surfactant-associated protein A
 Protein Family    Protein
 UniProt Gene Name    SFTPA1 [Similar Products]
 UniProt Synonym Gene Names    SFTP1; SFTPA; PSAP; PSP-A; SP-A [Similar Products]
 UniProt Entry Name    SFTPA_PIG
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 UniProt Comments for SPA    Function: In presence of calcium ions, PSAP binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration.

Subunit structure: Oligomeric complex of 6 set of homotrimers.

Subcellular location: Secreted › extracellular space › extracellular matrix. Secreted › extracellular space › surface film.

Miscellaneous: Pulmonary surfactant consists of 90% lipid and 10% protein. There are 4 surfactant-associated proteins: 2 collagenous, carbohydrate-binding glycoproteins (SP-A and SP-D) and 2 small hydrophobic proteins (SP-B and SP-C).

Sequence similarities: Belongs to the SFTPA family.Contains 1 C-type lectin domain.Contains 1 collagen-like domain.
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 Research Articles on SPA    1. Pulmonary surfactant-associated protein A (SP-A) is detected in thymus, trachea, lung, jejunum, and duodenum.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with SPA elisa kitDiseases associated with SPA elisa kit
 Products by Pathway  Pathway Diagram
 Pertussis Pathway antibodies  Pertussis Pathway Diagram
 Pertussis Pathway antibodies  Pertussis Pathway Diagram
 Phagosome Pathway antibodies  Phagosome Pathway Diagram
 Phagosome Pathway antibodies  Phagosome Pathway Diagram
 Disease Name  Pubmed Publications
 Lung Diseases Antibodies  >31 publications with SPA and Lung Diseases
 Inflammation Antibodies  >11 publications with SPA and Inflammation
 Fibrosis Antibodies  >7 publications with SPA and Fibrosis
 Pulmonary Fibrosis Antibodies  >7 publications with SPA and Pulmonary Fibrosis
 Lung Neoplasms Antibodies  >6 publications with SPA and Lung Neoplasms
 Neoplasms Antibodies  >6 publications with SPA and Neoplasms
 Pneumonia Antibodies  >4 publications with SPA and Pneumonia
 Necrosis Antibodies  >4 publications with SPA and Necrosis
 Lung Injury Antibodies  >4 publications with SPA and Lung Injury
 Idiopathic Pulmonary Fibrosis Antibodies  >3 publications with SPA and Idiopathic Pulmonary Fibrosis
Organs/Tissues associated with SPA elisa kit
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >71 publications with SPA and Lung
 Liver Antibodies  >1 publications with SPA and Liver
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