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RIPK3 elisa kit :: Mouse Receptor Interacting Serine Threonine Kinase 3 (RIPK3) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2025051 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $270 +1 FREE 8GB USB
48-Strip-Wells  /  $505 +1 FREE 8GB USB
96-Strip-Wells  /  $685 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,770 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,025 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Receptor Interacting Serine Threonine Kinase 3 (RIPK3), ELISA Kit

★Popular Item★
 Also Known As   

Receptor Interacting Serine Threonine Kinase 3 (RIPK3) ELISA Kit

 Product Synonym Names    RIP3; RIP-like protein kinase 3; Receptor-interacting protein 3
 Product Gene Name   

RIPK3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    605817
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Receptor Interacting Serine Threonine Kinase 3 (RIPK3).
No significant cross-reactivity or interference between Receptor Interacting Serine Threonine Kinase 3 (RIPK3) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Tissue homogenates, Cell lysates and Other Biological Fluids
 Detection Range    0.312-20ng/mL
 Sensitivity    < 0.112ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Receptor Interacting Serine Threonine Kinase 3 (RIPK3) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Receptor Interacting Serine Threonine Kinase 3 (RIPK3) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of RIPK3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for RIPK3 purchase    MBS2025051 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Receptor Interacting Serine Threonine Kinase 3 (RIPK3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312-20ng/mL in biological research samples containing RIPK3, with an estimated sensitivity of < 0.112ng/mL. The ELISA analytical biochemical technique of the MBS2025051 kit is based on RIPK3 antibody-RIPK3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RIPK3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RIPK3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates, Cell lysates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for RIPK3 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Receptor Interacting Serine Threonine Kinase 3 (RIPK3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Receptor Interacting Serine Threonine Kinase 3 (RIPK3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Receptor Interacting Serine Threonine Kinase 3 (RIPK3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Receptor Interacting Serine Threonine Kinase 3 (RIPK3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for RIPK3 elisa kit    Enzyme & Kinase
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 Typical Testing Data/Standard Curve (for reference only) of RIPK3 elisa kit    RIPK3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2025051. Click to request current manual
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NCBI/Uniprot data below describe general gene information for RIPK3. It may not necessarily be applicable to this product.
 NCBI GI #    38512228
 NCBI GeneID    11035
 NCBI Accession #    AAH62584.1 [Other Products]
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 UniProt Secondary Accession #    Q5J795; Q5J796; Q6P5Y1; B4DJL9; C4AM87 [Other Products]
 UniProt Related Accession #    Q9Y572 [Other Products]
 Molecular Weight    25,326 Da
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 NCBI Official Full Name    Receptor-interacting serine-threonine kinase 3
 NCBI Official Synonym Full Names    receptor-interacting serine-threonine kinase 3
 NCBI Official Symbol    RIPK3 [Similar Products]
 NCBI Official Synonym Symbols   
RIP3
[Similar Products]
 NCBI Protein Information    receptor-interacting serine/threonine-protein kinase 3
 UniProt Protein Name    Receptor-interacting serine/threonine-protein kinase 3
 UniProt Synonym Protein Names   
RIP-like protein kinase 3; Receptor-interacting protein 3; RIP-3
 Protein Family    Receptor-interacting serine/threonine-protein kinase
 UniProt Gene Name    RIPK3 [Similar Products]
 UniProt Synonym Gene Names    RIP3; RIP-3 [Similar Products]
 UniProt Entry Name    RIPK3_HUMAN
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 NCBI Summary for RIPK3    The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor. [provided by RefSeq, Jul 2008]
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 UniProt Comments for RIPK3    RIPK3: Promotes apoptosis. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Binds TRAF2 and RIPK1 and is recruited to the TNFR-1 signaling complex. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; EC 2.7.11.1; Kinase, protein; TKL group; RIPK family

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: mitochondrion; plasma membrane; cytosol

Molecular Function: NF-kappaB-inducing kinase activity; identical protein binding; protein serine/threonine kinase activity; protein binding; transcription coactivator activity; protein complex binding; ATP binding; protein kinase activity

Biological Process: spleen development; I-kappaB kinase/NF-kappaB cascade; regulation of activated T cell proliferation; thymus development; protein heterooligomerization; protein amino acid autophosphorylation; MyD88-independent toll-like receptor signaling pathway; T cell differentiation in the thymus; protein modification process; toll-like receptor 3 signaling pathway; signal transduction; lymph node development; activation of NF-kappaB transcription factor; regulation of T cell mediated cytotoxicity; regulation of adaptive immune response; regulation of interferon-gamma production; toll-like receptor signaling pathway; positive regulation of interferon type I production; activation of protein kinase activity; innate immune response; T cell homeostasis; positive regulation of ligase activity; toll-like receptor 4 signaling pathway; protein homooligomerization; positive regulation of oxidoreductase activity
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 Research Articles on RIPK3    1. Suppression of RIP3-dependent necroptosis by human cytomegalovirus
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with RIPK3 elisa kitPathways associated with RIPK3 elisa kit
 Reference Product  PubMed Publications
 RIPK1 elisa kit  >87 publications with RIPK3 and RIPK1
 MLKL elisa kit  >40 publications with RIPK3 and MLKL
 ZBP1 elisa kit  >5 publications with RIPK3 and ZBP1
 TLR3 elisa kit  >4 publications with RIPK3 and TLR3
 TICAM1 elisa kit  >3 publications with RIPK3 and TICAM1
 Products by Pathway  Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Cytosolic Sensors Of Pathogen-associated DNA Pathway antibodies  Cytosolic Sensors Of Pathogen-associated DNA Pathway Diagram
 IKK Complex Recruitment Mediated By RIP1 Pathway antibodies  IKK Complex Recruitment Mediated By RIP1 Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 MyD88-independent Cascade Pathway antibodies  MyD88-independent Cascade Pathway Diagram
 RIP-mediated NFkB Activation Via ZBP1 Pathway antibodies  RIP-mediated NFkB Activation Via ZBP1 Pathway Diagram
 TNF Signaling Pathway antibodies  TNF Signaling Pathway Diagram
Diseases associated with RIPK3 elisa kitOrgans/Tissues associated with RIPK3 elisa kit
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >174 publications with RIPK3 and Necrosis
 Inflammation Antibodies  >68 publications with RIPK3 and Inflammation
 Cardiovascular Diseases Antibodies  >12 publications with RIPK3 and Cardiovascular Diseases
 Liver Diseases Antibodies  >12 publications with RIPK3 and Liver Diseases
 Drug-Induced Liver Injury Antibodies  >6 publications with RIPK3 and Drug-Induced Liver Injury
 Lung Diseases Antibodies  >4 publications with RIPK3 and Lung Diseases
 Drug-Related Side Effects and Adverse Reactions Antibodies  >4 publications with RIPK3 and Drug-Related Side Effects and Adverse Reactions
 Liver Neoplasms Antibodies  >3 publications with RIPK3 and Liver Neoplasms
 Acute Kidney Injury Antibodies  >3 publications with RIPK3 and Acute Kidney Injury
 Carcinoma, Hepatocellular Antibodies  >2 publications with RIPK3 and Carcinoma, Hepatocellular
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >28 publications with RIPK3 and Blood
 Liver Antibodies  >19 publications with RIPK3 and Liver
 Intestine Antibodies  >11 publications with RIPK3 and Intestine
 Brain Antibodies  >9 publications with RIPK3 and Brain
 Kidney Antibodies  >8 publications with RIPK3 and Kidney
 Lung Antibodies  >8 publications with RIPK3 and Lung
 Heart Antibodies  >6 publications with RIPK3 and Heart
 Skin Antibodies  >5 publications with RIPK3 and Skin
 Vascular Antibodies  >3 publications with RIPK3 and Vascular
 Muscle Antibodies  >3 publications with RIPK3 and Muscle
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