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REG3g elisa kit :: Mouse Regenerating Islet Derived Protein 3 Gamma (REG3g) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS451182
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $545 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,480 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,820 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Regenerating Islet Derived Protein 3 Gamma (REG3g), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Regenerating Islet Derived Protein 3 Gamma (REG3g) ELISA Kit

 Product Synonym Names    REG-III; PAP1B; PAPIB; Pancreatitis-associated protein 1B; Regenerating islet-derived protein III-gamma
 Product Gene Name   

REG3g elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for O09049
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of REG3g. No significant cross-reactivity or interference between REG3g and analogues was observed.
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 Samples    Serum, plasma and other biological fluids.
 Assay Type    Sandwich
 Detection Range    15.625-1000pg/mL
 Sensitivity    6.9pg/mL
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level REG3g were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level REG3g were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
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 Preparation and Storage    For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of REG3g elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for REG3g purchase    MBS451182 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Regenerating Islet Derived Protein 3 Gamma (REG3g) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.625-1000pg/mL in biological research samples containing REG3g, with an estimated sensitivity of 6.9pg/mL. The ELISA analytical biochemical technique of the MBS451182 kit is based on REG3g antibody-REG3g antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect REG3g antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, REG3g. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for REG3g elisa kit

   Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of REG3g in mouse serum, plasma and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to REG3g. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to REG3g. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain REG3g, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of REG3g in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of REG3g elisa kit    REG3g elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS451182. Click to request current manual
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NCBI/Uniprot data below describe general gene information for REG3g. It may not necessarily be applicable to this product.
 NCBI GI #    6755310
 NCBI GeneID    19695
 NCBI Accession #    NP_035390.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_011260.2 [Other Products]
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 UniProt Primary Accession #    O09049 [Other Products]
 UniProt Related Accession #    O09049 [Other Products]
 Molecular Weight    19,307 Da
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 NCBI Official Full Name    regenerating islet-derived protein 3-gamma preproprotein
 NCBI Official Synonym Full Names    regenerating islet-derived 3 gamma
 NCBI Official Symbol    Reg3g [Similar Products]
 NCBI Official Synonym Symbols   
AI449515; REG-3-gamma; reg III-gamma
[Similar Products]
 NCBI Protein Information    regenerating islet-derived protein 3-gamma
 UniProt Protein Name    Regenerating islet-derived protein 3-gamma
 UniProt Synonym Protein Names   
Pancreatitis-associated protein 3; Regenerating islet-derived protein III-gamma; Reg III-gamma
 Protein Family    Regenerating islet-derived protein
 UniProt Gene Name    Reg3g [Similar Products]
 UniProt Synonym Gene Names    Pap3; REG-3-gamma; Reg III-gamma [Similar Products]
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 NCBI Summary for REG3g    This gene encodes a C-type lectin that demonstrates bactericidal activity. This gene is predominantly expressed in the distal small intestine where the encoded protein undergoes proteolytic processing by trypsin. Mice lacking the encoded protein exhibit altered mucus distribution, increased bacterial contact with the epithelium, and elevated inflammatory markers in the ileum, and low-grade inflammation. [provided by RefSeq, Jun 2016]
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 UniProt Comments for REG3g    Bactericidal C-type lectin which acts exclusively against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan. Restricts bacterial colonization of the intestinal epithelial surface and consequently limits activation of adaptive immune responses by the microbiota. The uncleaved form has bacteriostatic activity, whereas the cleaved form has bactericidal activity against L.monocytogenes and methicillin-resistant S.aureus. Regulates keratinocyte proliferation and differentiation after skin injury.
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 Research Articles on REG3g    1. Intestinal deficiency in REG3B or REG3G increases numbers of mucosa-associated bacteria and enhances bacterial translocation to the mesenteric lymph nodes and liver.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with REG3g elisa kitPathways associated with REG3g elisa kit
 Reference Product  PubMed Publications
 Reg3b elisa kit  >8 publications with REG3g and Reg3b
 Cbr2 elisa kit  >1 publications with REG3g and Cbr2
 Products by Pathway  Pathway Diagram
 Antimicrobial Peptides Pathway antibodies  Antimicrobial Peptides Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
Diseases associated with REG3g elisa kitOrgans/Tissues associated with REG3g elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >26 publications with REG3g and Inflammation
 Disease Models, Animal Antibodies  >15 publications with REG3g and Disease Models, Animal
 Necrosis Antibodies  >5 publications with REG3g and Necrosis
 Fatty Liver Antibodies  >3 publications with REG3g and Fatty Liver
 Lung Diseases Antibodies  >3 publications with REG3g and Lung Diseases
 Immune System Diseases Antibodies  >3 publications with REG3g and Immune System Diseases
 Liver Diseases Antibodies  >3 publications with REG3g and Liver Diseases
 Pneumonia Antibodies  >2 publications with REG3g and Pneumonia
 Weight Loss Antibodies  >2 publications with REG3g and Weight Loss
 Cardiovascular Diseases Antibodies  >2 publications with REG3g and Cardiovascular Diseases
 Organ/Tissue Name  Pubmed Publications
 Intestine Antibodies  >45 publications with REG3g and Intestine
 Pancreas Antibodies  >9 publications with REG3g and Pancreas
 Stomach Antibodies  >2 publications with REG3g and Stomach
 Thymus Antibodies  >2 publications with REG3g and Thymus
 Heart Antibodies  >1 publications with REG3g and Heart
 Embryonic Tissue Antibodies  >1 publications with REG3g and Embryonic Tissue
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