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REN elisa kit :: Mouse Renin-1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS177247
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96-Strip-Wells  /  $425 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Renin-1, ELISA Kit

 Also Known As   

Mouse Renin-1 PicoKine ELISA Kit

 Product Synonym Names    Renin-1; renin; Angiotensin forming nzyme; Angiotensinogenase; FLJ10761; HNFJ2; REN; Ren1; RENAA; RENI_HUMAN; Renin; Renin precursor renal; Renin-1; Renin-2
 Product Gene Name   

REN elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for P06281
 Species Reactivity    Mouse
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 Specificity    Natural and recombinant mouse Renin-1
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 Samples    Cell culture supernates, serum and plasma (heparin, EDTA)
 Assay Type    Sandwich
 Detection Range    62.5pg/ml-4000pg/ml
 Sensitivity    < 10pg/ml
 Intra-assay Precision    Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
 Inter-assay Precision    Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
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 Preparation and Storage    Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of REN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for REN purchase    MBS177247 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Renin-1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 62.5pg/ml-4000pg/ml in biological research samples containing REN, with an estimated sensitivity of < 10pg/ml. The ELISA analytical biochemical technique of the MBS177247 kit is based on REN antibody-REN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect REN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, REN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Cell culture supernates, serum and plasma (heparin, EDTA). Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for REN elisa kit

   Principle of the assay: mouse Renin-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Renin-1 has been precoated onto 96-well plates. Standards(NSO, S72-R402) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Renin-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Renin-1 amount of sample captured in plate.
Background: Renin-1 is also known as Ren, Angiotensinogenase or Kidney rennin. It is a member of peptidase A1 family. Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. It associated to membranes via binding to ATP6AP2.
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 Typical Testing Data/Standard Curve (for reference only) of REN elisa kit    Mouse Renin-1 PicoKine ELISA Kit standard curve
REN elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS177247. Click to request current manual
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NCBI/Uniprot data below describe general gene information for REN. It may not necessarily be applicable to this product.
 NCBI GI #    132327
 NCBI GeneID    19701
 NCBI Accession #    P06281.1 [Other Products]
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 UniProt Primary Accession #    P06281 [Other Products]
 UniProt Secondary Accession #    P97911; Q543E5; Q62153; Q62154 [Other Products]
 UniProt Related Accession #    P06281 [Other Products]
 Molecular Weight    44,343 Da
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 NCBI Official Full Name    Renin-1
 NCBI Official Synonym Full Names    renin 1 structural
 NCBI Official Symbol    Ren1 [Similar Products]
 NCBI Official Synonym Symbols   
Ren; Rnr; Rn-1; Ren-1; Ren-A; Ren1c; Ren1d; D19352
[Similar Products]
 NCBI Protein Information    renin-1; renin b; kidney renin; aspartyl-protease; angiotensinogenase
 UniProt Protein Name    Renin-1
 UniProt Synonym Protein Names   
Angiotensinogenase; Kidney renin
 Protein Family    Renin
 UniProt Gene Name    Ren1 [Similar Products]
 UniProt Synonym Gene Names    Ren; Ren-1 [Similar Products]
 UniProt Entry Name    RENI1_MOUSE
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 UniProt Comments for REN    REN: Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. Interacts with ATP6AP2. Interaction with ATP6AP2 results in a 5-fold increased efficiency in angiotensinogen processing. Belongs to the peptidase A1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; EC 3.4.23.15; Protease; Secreted, signal peptide

Cellular Component: extracellular space; membrane; cytoplasm; extracellular region; intracellular

Molecular Function: peptidase activity; insulin-like growth factor receptor binding; hydrolase activity; endopeptidase activity; aspartic-type endopeptidase activity; receptor binding

Biological Process: response to drug; response to cAMP; establishment of blood-nerve barrier; hormone-mediated signaling; cell maturation; renin-angiotensin regulation of aldosterone production; drinking behavior; angiotensin maturation; proteolysis; response to organic substance; regulation of MAPKKK cascade; regulation of blood pressure; renin-angiotensin regulation of blood volume; kidney development
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 Research Articles on REN    1. Data indicate the role of renin in hypertension linked to loss of connexin 40 (Cx40).
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with REN elisa kitDiseases associated with REN elisa kit
 Products by Pathway  Pathway Diagram
 ACE Inhibitor Pathway antibodies  ACE Inhibitor Pathway Diagram
 Metabolism Of Angiotensinogen To Angiotensins Pathway antibodies  Metabolism Of Angiotensinogen To Angiotensins Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Peptide Hormone Metabolism Pathway antibodies  Peptide Hormone Metabolism Pathway Diagram
 Renin-angiotensin System Pathway antibodies  Renin-angiotensin System Pathway Diagram
 Renin-angiotensin System Pathway antibodies  Renin-angiotensin System Pathway Diagram
 Disease Name  Pubmed Publications
 Hypertension Antibodies  >5 publications with REN and Hypertension
 Cardiovascular Diseases Antibodies  >4 publications with REN and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >4 publications with REN and Disease Models, Animal
 Kidney Diseases Antibodies  >3 publications with REN and Kidney Diseases
 Neoplasms, Experimental Antibodies  >1 publications with REN and Neoplasms, Experimental
 Kidney Neoplasms Antibodies  >1 publications with REN and Kidney Neoplasms
 Hemorrhage Antibodies  >1 publications with REN and Hemorrhage
 Fibrosis Antibodies  >1 publications with REN and Fibrosis
 Inflammation Antibodies  >1 publications with REN and Inflammation
Organs/Tissues associated with REN elisa kit
 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >16 publications with REN and Kidney
 Brain Antibodies  >4 publications with REN and Brain
 Salivary Gland Antibodies  >3 publications with REN and Salivary Gland
 Liver Antibodies  >1 publications with REN and Liver
 Testis Antibodies  >1 publications with REN and Testis
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