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RPE65 elisa kit :: Mouse Retinoid isomerohydrolase (RPE65) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7214137
Typical Testing Data/Standard Curve
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Retinoid isomerohydrolase (RPE65), ELISA Kit

 Also Known As   

Mouse Retinoid isomerohydrolase (RPE65) ELISA Kit

 Product Gene Name   

RPE65 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of RPE65. No significant cross-reactivity or interference between RPE65 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RPE65 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    0.1 ng/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of RPE65 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for RPE65 purchase    MBS7214137 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Retinoid isomerohydrolase (RPE65) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing RPE65, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS7214137 kit is based on RPE65 antibody-RPE65 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RPE65 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RPE65. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for RPE65 elisa kit

   Intended Uses: This RPE65 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse RPE65. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay||RPE65 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RPE65 antibody and an RPE65-HRP conjugate. The assay sample and buffer are incubated together with RPE65-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RPE65 concentration since RPE65 from samples and RPE65-HRP conjugate compete for the anti-RPE65 antibody binding site. Since the number of sites is limited, as more sites are occupied by RPE65 from the sample, fewer sites are left to bind RPE65-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RPE65 concentration in each sample is interpolated from this standard curve.
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 Typical Testing Data/Standard Curve of RPE65 elisa kit    RPE65 elisa kit Typical Testing Data/Standard Curve image
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Sample Manual Insert of MBS7214137
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NCBI/Uniprot data below describe general gene information for RPE65. It may not necessarily be applicable to this product.
 NCBI GI #    45385809
 NCBI GeneID    395700
 NCBI Accession #    NP_990215.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_204884.1 [Other Products]
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 UniProt Related Accession #    Q9YGX2 [Other Products]
 Molecular Weight    60,930 Da
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 NCBI Official Full Name    retinoid isomerohydrolase
 NCBI Official Synonym Full Names    retinal pigment epithelium-specific protein 65kDa
 NCBI Official Symbol    RPE65 [Similar Products]
 NCBI Protein Information    retinoid isomerohydrolase; retinol isomerase; all-trans-retinyl-palmitate hydrolase; retinal pigment epithelium-specific 65 kDa protein
 UniProt Protein Name    Retinoid isomerohydrolase
 UniProt Synonym Protein Names   
All-trans-retinyl-palmitate hydrolase; Retinal pigment epithelium-specific 65 kDa protein; Retinol isomerase
 UniProt Gene Name    RPE65 [Similar Products]
 UniProt Entry Name    RPE65_CHICK
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 UniProt Comments for RPE65    Plays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. The enzymatic activity is linearly dependent of the expression levels and membrane association.
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 Research Articles on RPE65    1. RPE65 undergoes conformational changes in secondary and tertiary structures upon binding with the phospholipid membrane. These conformational rearrangements facilitate restoration of a catalytically active state of RPE65.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with RPE65 elisa kitPathways associated with RPE65 elisa kit
 Reference Product  PubMed Publications
 LRAT elisa kit  >47 publications with RPE65 and LRAT
 RDH12 elisa kit  >14 publications with RPE65 and RDH12
 RDH5 elisa kit  >5 publications with RPE65 and RDH5
 RLBP1 elisa kit  >4 publications with RPE65 and RLBP1
 RPGR elisa kit  >3 publications with RPE65 and RPGR
 RDH10 elisa kit  >3 publications with RPE65 and RDH10
 Products by Pathway  Pathway Diagram
 Retinol Metabolism Pathway antibodies  Retinol Metabolism Pathway Diagram
 Retinol Metabolism Pathway antibodies  Retinol Metabolism Pathway Diagram
 Retinol Metabolism (BiGCaT, NuGO) Pathway antibodies  Retinol Metabolism (BiGCaT, NuGO) Pathway Diagram
Diseases associated with RPE65 elisa kitOrgans/Tissues associated with RPE65 elisa kit
 Disease Name  Pubmed Publications
 Leber Congenital Amaurosis Antibodies  >157 publications with RPE65 and Leber Congenital Amaurosis
 Eye Diseases, Hereditary Antibodies  >131 publications with RPE65 and Eye Diseases, Hereditary
 Blindness Antibodies  >116 publications with RPE65 and Blindness
 Disease Models, Animal Antibodies  >86 publications with RPE65 and Disease Models, Animal
 Retinal Dystrophies Antibodies  >66 publications with RPE65 and Retinal Dystrophies
 Retinitis Pigmentosa Antibodies  >62 publications with RPE65 and Retinitis Pigmentosa
 Macular Degeneration Antibodies  >53 publications with RPE65 and Macular Degeneration
 Optic Atrophy, Hereditary, Leber Antibodies  >32 publications with RPE65 and Optic Atrophy, Hereditary, Leber
 Nerve Degeneration Antibodies  >16 publications with RPE65 and Nerve Degeneration
 Brain Diseases Antibodies  >7 publications with RPE65 and Brain Diseases
 Organ/Tissue Name  Pubmed Publications
 Head Antibodies  >10 publications with RPE65 and Head
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