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S100A12 elisa kit :: Mouse S100 Calcium Binding Protein A12 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS728991
Typical Testing Data/Standard Curve
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

S100 Calcium Binding Protein A12 (S100A12), ELISA Kit

★Popular Item★
 Also Known As   

Mouse S100 Calcium Binding Protein A12 ELISA Kit

 Product Gene Name   

S100A12 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    603112
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of S100A12. No significant cross-reactivity or interference between S100A12 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between S100A12 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Sandwich
 Detection Range    50-1000pg/mL
 Sensitivity    1.0pg/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of S100A12 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for S100A12 purchase    MBS728991 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the S100 Calcium Binding Protein A12 (S100A12) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 50-1000pg/mL in biological research samples containing S100A12, with an estimated sensitivity of 1.0pg/mL. The ELISA analytical biochemical technique of the MBS728991 kit is based on S100A12 antibody-S100A12 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect S100A12 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, S100A12. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for S100A12 elisa kit

   Intended Uses: This S100A12 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse S100A12. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay||S100A12 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for S100A12. Standards or samples are then added to the microtiter plate wells and S100A12 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of S100A12 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for S100A12 are added to each well to "sandwich" the S100A12 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain S100A12 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The S100A12 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for S100A12 elisa kit    Signal Transduction
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 Typical Testing Data/Standard Curve of S100A12 elisa kit    S100A12 elisa kit Typical Testing Data/Standard Curve image
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Sample Manual Insert of MBS728991. Click to request current manual
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NCBI/Uniprot data below describe general gene information for S100A12. It may not necessarily be applicable to this product.
 NCBI GI #    119573717
 NCBI GeneID    6283
 NCBI Accession #    EAW53332.1 [Other Products]
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 UniProt Secondary Accession #    P83219; Q5SY66; Q7M4R1 [Other Products]
 UniProt Related Accession #    P80511 [Other Products]
 Molecular Weight    10,575 Da
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 NCBI Official Full Name    S100 calcium binding protein A12 (calgranulin C)
 NCBI Official Synonym Full Names    S100 calcium binding protein A12
 NCBI Official Symbol    S100A12 [Similar Products]
 NCBI Official Synonym Symbols   
p6; CAGC; CGRP; MRP6; CAAF1; MRP-6; ENRAGE
[Similar Products]
 NCBI Protein Information    protein S100-A12; EN-RAGE; calgranulin C; calgranulin-C; neutrophil S100 protein; calcium-binding protein in amniotic fluid 1; migration inhibitory factor-related protein 6; S100 calcium-binding protein A12 (calgranulin C); extracellular newly identified RAGE-binding protein
 UniProt Protein Name    Protein S100-A12
 UniProt Synonym Protein Names   
CGRP; Calcium-binding protein in amniotic fluid 1; CAAF1; Calgranulin-C; CAGC; Extracellular newly identified RAGE-binding protein; EN-RAGE; Migration inhibitory factor-related protein 6; MRP-6; p6; Neutrophil S100 protein; S100 calcium-binding protein A12Cleaved into the following chain:Calcitermin
 Protein Family    Protein
 UniProt Gene Name    S100A12 [Similar Products]
 UniProt Synonym Gene Names    CAAF1; CAGC; EN-RAGE; MRP-6; p6 [Similar Products]
 UniProt Entry Name    S10AC_HUMAN
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 NCBI Summary for S100A12    The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities. [provided by RefSeq, Jul 2008]
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 UniProt Comments for S100A12    S100A12: S100A12 is a calcium-, zinc- and copper-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. Its proinflammatory activity involves recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to receptor for advanced glycation endproducts (AGER). Binding to AGER activates the MAP-kinase and NF-kappa-B signaling pathways leading to production of proinflammatory cytokines and up-regulation of cell adhesion molecules ICAM1 and VCAM1. Acts as a monocyte and mast cell chemoattractant. Can stimulate mast cell degranulation and activation which generates chemokines, histamine and cytokines inducing further leukocyte recruitment to the sites of inflammation. Can inhibit the activity of matrix metalloproteinases; MMP2, MMP3 and MMP9 by chelating Zn(2+) from their active sites. Possesses filariacidal and filariastatic activity. Calcitermin possesses antifungal activity against C.albicans and is also active against E.coli and P.aeruginosa but not L.monocytogenes and S.aureus. Belongs to the S-101 family.

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: cytoskeleton; cytoplasm; extracellular region; plasma membrane; cytosol; nucleus

Molecular Function: protein binding; RAGE receptor binding; copper ion binding; zinc ion binding; calcium ion binding

Biological Process: neutrophil chemotaxis; positive regulation of I-kappaB kinase/NF-kappaB cascade; killing of cells of another organism; mast cell activation; activation of NF-kappaB transcription factor; monocyte chemotaxis; positive regulation of MAP kinase activity; xenobiotic metabolic process; defense response to bacterium; innate immune response; inflammatory response; defense response to fungus; cytokine secretion; positive regulation of inflammatory response
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 Research Articles on S100A12    1. Excessive expression of the S100A12 gene in uremic leukocytes is relevant to its increased serum concentration, particularly in those affected with cardiovascular disease.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with S100A12 elisa kitPathways associated with S100A12 elisa kit
 Reference Product  PubMed Publications
 S100A8 elisa kit  >98 publications with S100A12 and S100A8
 S100A9 elisa kit  >83 publications with S100A12 and S100A9
 CACYBP elisa kit  >2 publications with S100A12 and CACYBP
 AGER elisa kit  >1 publications with S100A12 and AGER
 FPR1 elisa kit  >1 publications with S100A12 and FPR1
 Products by Pathway  Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Advanced Glycosylation Endproduct Receptor Signaling Pathway antibodies  Advanced Glycosylation Endproduct Receptor Signaling Pathway Diagram
 Cytosolic Sensors Of Pathogen-associated DNA Pathway antibodies  Cytosolic Sensors Of Pathogen-associated DNA Pathway Diagram
 DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway antibodies  DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 MyD88 Cascade Initiated On Plasma Membrane Pathway antibodies  MyD88 Cascade Initiated On Plasma Membrane Pathway Diagram
 MyD88 Dependent Cascade Initiated On Endosome Pathway antibodies  MyD88 Dependent Cascade Initiated On Endosome Pathway Diagram
 MyD88-independent Cascade Pathway antibodies  MyD88-independent Cascade Pathway Diagram
 MyD88:Mal Cascade Initiated On Plasma Membrane Pathway antibodies  MyD88:Mal Cascade Initiated On Plasma Membrane Pathway Diagram
Diseases associated with S100A12 elisa kitOrgans/Tissues associated with S100A12 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >164 publications with S100A12 and Inflammation
 Immune System Diseases Antibodies  >48 publications with S100A12 and Immune System Diseases
 Gastrointestinal Diseases Antibodies  >43 publications with S100A12 and Gastrointestinal Diseases
 Cardiovascular Diseases Antibodies  >39 publications with S100A12 and Cardiovascular Diseases
 Necrosis Antibodies  >25 publications with S100A12 and Necrosis
 Atherosclerosis Antibodies  >20 publications with S100A12 and Atherosclerosis
 Diabetes Mellitus Antibodies  >11 publications with S100A12 and Diabetes Mellitus
 Nervous System Diseases Antibodies  >11 publications with S100A12 and Nervous System Diseases
 Bone Diseases Antibodies  >8 publications with S100A12 and Bone Diseases
 Carcinoma Antibodies  >7 publications with S100A12 and Carcinoma
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >140 publications with S100A12 and Blood
 Kidney Antibodies  >15 publications with S100A12 and Kidney
 Intestine Antibodies  >15 publications with S100A12 and Intestine
 Heart Antibodies  >8 publications with S100A12 and Heart
 Liver Antibodies  >3 publications with S100A12 and Liver
 Spleen Antibodies  >3 publications with S100A12 and Spleen
 Bone Marrow Antibodies  >3 publications with S100A12 and Bone Marrow
 Adipose Tissue Antibodies  >1 publications with S100A12 and Adipose Tissue
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