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S100A9 elisa kit :: Mouse S100 Calcium Binding Protein A9 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS762309
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $280 +1 FREE 8GB USB
96-Strip-Wells  /  $375 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,015 +3 FREE 8GB USB
15x96-Strip-Wells  /  $4,285 +5 FREE 8GB USB
25x96-Strip-Wells  /  $6,715 +8 FREE 8GB USB
 
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 Product Name   

S100 Calcium Binding Protein A9 (S100A9), ELISA Kit

★Popular Item★
 Also Known As   

Mouse S100 Calcium Binding Protein A9 ELISA Kit

 Product Synonym Names    S100A9/Calgranulin B/MRP-14/CAGBMigration inhibitory factor-related protein 14/calgranulin B/calgranulin-B/Calgranulin-B/Calprotectin L1H subunit/CFAGMRP-14,60B8AG/CGLB/L1AG/LIAG/MAC387/MIF/MRP14Leukocyte L1 complex heavy chain/NIF/P14/p14/protein S100-A9/S100 calcium binding protein A9/S100 calcium binding protein A9 (calgranulin B)/S100 calcium-binding protein A9/S100 calcium-binding protein A9 (calgranulin B)
 Product Gene Name   

S100A9 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS762309 COA    COA PDF
 OMIM    123886
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of S100A9. No significant cross-reactivity or interference between S100A9 and analogues was observed.
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 Samples    Serum, plasma and other biological fluids.
 Assay Type    Sandwich ELISA, Double Antibody
 Detection Range    0.156-10ng/ml
 Sensitivity    0.094ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level S100A9 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level S100A9 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of S100A9 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for S100A9 purchase    MBS762309 is a ready-to-use microwell, strip plate Sandwich, Double Antibody ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the S100 Calcium Binding Protein A9 (S100A9) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/ml in biological research samples containing S100A9, with an estimated sensitivity of 0.094ng/ml. The ELISA analytical biochemical technique of the MBS762309 kit is based on S100A9 antibody-S100A9 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect S100A9 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, S100A9. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for S100A9 elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- S100A9 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- S100A9 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the S100A9 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of S100A9 can be calculated.
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 Typical Testing Data/Standard Curve (for reference only) of S100A9 elisa kit    S100A9 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS762309. Click to request current manual
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NCBI/Uniprot data below describe general gene information for S100A9. It may not necessarily be applicable to this product.
 NCBI GI #    29126810
 NCBI GeneID    6280
 NCBI Accession #    AAH47681.1 [Other Products]
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 UniProt Secondary Accession #    Q6FGA1; Q9NYM0; Q9UCJ1; D3DV36 [Other Products]
 UniProt Related Accession #    P06702 [Other Products]
 Molecular Weight    13,242 Da
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 NCBI Official Full Name    S100 calcium binding protein A9
 NCBI Official Synonym Full Names    S100 calcium binding protein A9
 NCBI Official Symbol    S100A9 [Similar Products]
 NCBI Official Synonym Symbols   
MIF; NIF; P14; CAGB; CFAG; CGLB; L1AG; LIAG; MRP14; 60B8AG; MAC387
[Similar Products]
 NCBI Protein Information    protein S100-A9
 UniProt Protein Name    Protein S100-A9
 UniProt Synonym Protein Names   
Calgranulin-B; Calprotectin L1H subunit; Leukocyte L1 complex heavy chain; Migration inhibitory factor-related protein 14; MRP-14; p14; S100 calcium-binding protein A9
 Protein Family    Protein
 UniProt Gene Name    S100A9 [Similar Products]
 UniProt Synonym Gene Names    CAGB; CFAG; MRP14; MRP-14; p14 [Similar Products]
 UniProt Entry Name    S10A9_HUMAN
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 NCBI Summary for S100A9    The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis. This antimicrobial protein exhibits antifungal and antibacterial activity. [provided by RefSeq, Nov 2014]
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 UniProt Comments for S100A9    S100A9: a calcium-binding regulatory protein of the S-100 family expressed by macrophages in acutely inflammated tissues and in chronic inflammations. May be an inhibitor of protein kinases. Also expressed in epithelial cells constitutively or induced during dermatoses. May interact with components of the intermediate filaments in monocytes and epithelial cells. Interacts with CEACAM3 in a calcium-dependent manner.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: cytoskeleton; cytosol; extracellular region; extracellular space; nucleus; plasma membrane

Molecular Function: antioxidant activity; arachidonic acid binding; calcium ion binding; microtubule binding; protein binding; RAGE receptor binding; signal transducer activity; zinc ion binding

Biological Process: actin cytoskeleton reorganization; activation of NF-kappaB transcription factor; astrocyte development; autophagy; caspase activation; cell-cell signaling; chemokine production; cytokine production; defense response to bacterium; defense response to fungus; inflammatory response; innate immune response; leukocyte migration during inflammatory response; neutrophil chemotaxis; positive regulation of blood coagulation; positive regulation of cell growth; positive regulation of inflammatory response; positive regulation of peptide secretion; regulation of cytoskeleton organization and biogenesis; regulation of integrin biosynthetic process; regulation of translation; sequestering of zinc ion; signal transduction
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 Research Articles on S100A9    1. Calprotectin levels correlated positively, though weakly, with platelet aggregation and activation.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with S100A9 elisa kitDiseases associated with S100A9 elisa kit
 Reference Product  PubMed Publications
 S100A8 elisa kit  >821 publications with S100A9 and S100A8
 TLR4 elisa kit  >64 publications with S100A9 and TLR4
 MYD88 elisa kit  >11 publications with S100A9 and MYD88
 MIF elisa kit  >4 publications with S100A9 and MIF
 PARP1 elisa kit  >2 publications with S100A9 and PARP1
 TIRAP elisa kit  >2 publications with S100A9 and TIRAP
 MYB elisa kit  >1 publications with S100A9 and MYB
 LY96 elisa kit  >1 publications with S100A9 and LY96
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >455 publications with S100A9 and Inflammation
 Disease Models, Animal Antibodies  >115 publications with S100A9 and Disease Models, Animal
 Lung Diseases Antibodies  >92 publications with S100A9 and Lung Diseases
 Nervous System Diseases Antibodies  >90 publications with S100A9 and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >84 publications with S100A9 and Cardiovascular Diseases
 Necrosis Antibodies  >82 publications with S100A9 and Necrosis
 Brain Diseases Antibodies  >55 publications with S100A9 and Brain Diseases
 Fibrosis Antibodies  >53 publications with S100A9 and Fibrosis
 Heart Diseases Antibodies  >36 publications with S100A9 and Heart Diseases
 Neoplasms, Experimental Antibodies  >33 publications with S100A9 and Neoplasms, Experimental
Organs/Tissues associated with S100A9 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >514 publications with S100A9 and Blood
 Skin Antibodies  >123 publications with S100A9 and Skin
 Lung Antibodies  >119 publications with S100A9 and Lung
 Vascular Antibodies  >96 publications with S100A9 and Vascular
 Brain Antibodies  >63 publications with S100A9 and Brain
 Bone Antibodies  >60 publications with S100A9 and Bone
 Liver Antibodies  >56 publications with S100A9 and Liver
 Muscle Antibodies  >47 publications with S100A9 and Muscle
 Bone Marrow Antibodies  >47 publications with S100A9 and Bone Marrow
 Kidney Antibodies  >46 publications with S100A9 and Kidney
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