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SOST elisa kit :: Mouse Sclerostin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS824629
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96 Tests  /  $470 +1 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Sclerostin (SOST), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Sclerostin ELISA Kit

 Product Synonym Names    Sclerostin
 Product Gene Name   

SOST elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for Q99P68
 Species Reactivity    Mouse
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 Specificity    The Mouse Sclerostin ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse Sclerostin proteins within the range of 15.6 pg/ml - 1000 pg/ml.
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 Samples    Cell Culture Supernatants, Serum, Plasma.
 Sensitivity    10 pg/ml.
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 Preparation and Storage    Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of SOST elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for SOST purchase    MBS824629 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Sclerostin (SOST) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SOST, with an estimated sensitivity of 10 pg/ml. The ELISA analytical biochemical technique of the MBS824629 kit is based on SOST antibody-SOST antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SOST antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SOST. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Cell Culture Supernatants, Serum, Plasma. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for SOST elisa kit

   Principle of the Assay: The Mouse Sclerostin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Sclerostin in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse Sclerostin coated on a 96-well plate. Standards and samples are pipetted into the wells and Sclerostin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Sclerostin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Sclerostin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Sclerostin is a protein that in humans is encoded by the SOST gene. Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Sclerostin is produced by the osteocyte and has anti-anabolic effects on bone formation. The SOST gene maps to chromosome 17q12-q21
 Applications Tested/Suitable for SOST elisa kit   

Sandwich ELISA (SE)

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 Typical Testing Data/Standard Curve (for reference only) of SOST elisa kit    SOST elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS824629. Click to request current manual
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NCBI/Uniprot data below describe general gene information for SOST. It may not necessarily be applicable to this product.
 NCBI GI #    567316045
 NCBI GeneID    74499
 NCBI Accession #    NP_077769.4 [Other Products]
 NCBI GenBank Nucleotide #    NM_024449.6 [Other Products]
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 UniProt Primary Accession #    Q99P68 [Other Products]
 UniProt Secondary Accession #    Q9D3L7 [Other Products]
 UniProt Related Accession #    Q99P68 [Other Products]
 Molecular Weight    23,443 Da
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 NCBI Official Full Name    sclerostin
 NCBI Official Synonym Full Names    sclerostin
 NCBI Official Symbol    Sost [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    sclerostin
 UniProt Protein Name    Sclerostin
 Protein Family    Sclerostin
 UniProt Gene Name    Sost [Similar Products]
 UniProt Entry Name    SOST_MOUSE
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 UniProt Comments for SOST    SOST: Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation. Defects in SOST are the cause of sclerosteosis type 1 (SOST1). An autosomal recessive sclerosing bone dysplasia characterized by a generalized hyperostosis and sclerosis leading to a markedly thickened skull, with mandible, ribs, clavicles and all long bones also being affected. Due to narrowing of the foramina of the cranial nerves, facial nerve palsy, hearing loss and atrophy of the optic nerves can occur. Sclerosteosis is clinically and radiologically very similar to van Buchem disease, mainly differentiated by hand malformations and a large stature in sclerosteosis patients. Defects in SOST are a cause of van Buchem disease (VBCH). An autosomal recessive sclerosing bone dysplasia characterized by endosteal hyperostosis of the mandible, skull, ribs, clavicles, and diaphyses of the long bones. Affected patients present a symmetrically increased thickness of bones, most frequently found as an enlarged jawbone, but also an enlargement of the skull, ribs, diaphysis of long bones, as well as tubular bones of hands and feet. The clinical consequence of increased thickness of the skull include facial nerve palsy causing hearing loss, visual problems, neurological pain, and, very rarely, blindness as a consequence of optic atrophy. Serum alkaline phosphatase levels are elevated. A 52 kb deletion downstream of SOST results in SOST transcription suppression causing van Buchem disease. Defects in SOST are a cause of craniodiaphyseal dysplasia autosomal dominant (CDD). A severe bone dysplasia characterized by massive generalized hyperostosis and sclerosis, especially involving the skull and facial bones. The sclerosis is so severe that the resulting facial distortion is referred to as 'leontiasis ossea' (leonine faces) and the bone deposition results in progressive stenosis of craniofacial foramina. Respiratory obstruction due to choanal stenosis compromises the clinical outcomes of affected patients. Heterozygous mutations located in the secretion signal of the SOST gene prevent sclerostin secretion and can be responsible for craniodiaphyseal dysplasia. Belongs to the sclerostin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular matrix; Golgi apparatus; extracellular space; proteinaceous extracellular matrix; extracellular region

Molecular Function: heparin binding; protein binding; transcription factor binding

Biological Process: ossification; Wnt receptor signaling pathway; negative regulation of Wnt receptor signaling pathway; positive regulation of transcription, DNA-dependent; negative regulation of ossification; negative regulation of protein complex assembly; negative regulation of BMP signaling pathway
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 Research Articles on SOST    1. Data indicate that low-dose parathyroid hormone (PTH) decreased the expression of the myocyte enhancer factor 2C (Mef2c) transcription factor, resulting in decreased sclerostin (Sost) expression in osteoblasts/osteocytes.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with SOST elisa kitDiseases associated with SOST elisa kit
 Products by Pathway  Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 RNF Mutants Show Enhanced WNT Signaling And Proliferation Pathway antibodies  RNF Mutants Show Enhanced WNT Signaling And Proliferation Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By WNT In Cancer Pathway antibodies  Signaling By WNT In Cancer Pathway Diagram
 Signaling By Wnt Pathway antibodies  Signaling By Wnt Pathway Diagram
 TCF Dependent Signaling In Response To WNT Pathway antibodies  TCF Dependent Signaling In Response To WNT Pathway Diagram
 Wnt Signaling Pathway antibodies  Wnt Signaling Pathway Diagram
 Wnt Signaling Pathway antibodies  Wnt Signaling Pathway Diagram
 XAV939 Inhibits Tankyrase, Stabilizing AXIN Pathway antibodies  XAV939 Inhibits Tankyrase, Stabilizing AXIN Pathway Diagram
 Misspliced LRP5 Mutants Have Enhanced Beta-catenin-dependent Signaling Pathway antibodies  Misspliced LRP5 Mutants Have Enhanced Beta-catenin-dependent Signaling Pathway Diagram
 Disease Name  Pubmed Publications
 Sclerosteosis Antibodies  >67 publications with SOST and Sclerosteosis
 Osteochondrodysplasias Antibodies  >30 publications with SOST and Osteochondrodysplasias
 Inflammation Antibodies  >20 publications with SOST and Inflammation
 Necrosis Antibodies  >17 publications with SOST and Necrosis
 Congenital Abnormalities Antibodies  >14 publications with SOST and Congenital Abnormalities
 Nervous System Diseases Antibodies  >13 publications with SOST and Nervous System Diseases
 Renal Insufficiency Antibodies  >12 publications with SOST and Renal Insufficiency
 Growth Disorders Antibodies  >9 publications with SOST and Growth Disorders
 Weight Loss Antibodies  >8 publications with SOST and Weight Loss
 Craniofacial Abnormalities Antibodies  >7 publications with SOST and Craniofacial Abnormalities
Organs/Tissues associated with SOST elisa kit
 Organ/Tissue Name  Pubmed Publications
 Inner Ear Antibodies  >1 publications with SOST and Inner Ear
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