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SEMA3A elisa kit :: Mouse Semaphorin-3A (SEMA3A) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7251267
Typical Testing Data/Standard Curve
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Semaphorin-3A (SEMA3A), ELISA Kit

 Also Known As   

Mouse Semaphorin-3A (SEMA3A) ELISA Kit

 Product Gene Name   

SEMA3A elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    gene 614897
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of SEMA3A. No significant cross-reactivity or interference between SEMA3A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SEMA3A and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    0.1 ng/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of SEMA3A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for SEMA3A purchase    MBS7251267 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Semaphorin-3A (SEMA3A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SEMA3A, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS7251267 kit is based on SEMA3A antibody-SEMA3A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SEMA3A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SEMA3A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for SEMA3A elisa kit

   Intended Uses: This SEMA3A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse SEMA3A. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay||SEMA3A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SEMA3A antibody and an SEMA3A-HRP conjugate. The assay sample and buffer are incubated together with SEMA3A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SEMA3A concentration since SEMA3A from samples and SEMA3A-HRP conjugate compete for the anti-SEMA3A antibody binding site. Since the number of sites is limited, as more sites are occupied by SEMA3A from the sample, fewer sites are left to bind SEMA3A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SEMA3A concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for SEMA3A elisa kit    Cardiovascular
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 Typical Testing Data/Standard Curve of SEMA3A elisa kit    SEMA3A elisa kit Typical Testing Data/Standard Curve image
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Sample Manual Insert of MBS7251267. Click to request current manual
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NCBI/Uniprot data below describe general gene information for SEMA3A. It may not necessarily be applicable to this product.
 NCBI GI #    5174673
 NCBI GeneID    10371
 NCBI Accession #    NP_006071.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_006080.2 [Other Products]
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 UniProt Related Accession #    Q14563 [Other Products]
 Molecular Weight    88,889 Da
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 NCBI Official Full Name    semaphorin-3A
 NCBI Official Synonym Full Names    sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
 NCBI Official Symbol    SEMA3A [Similar Products]
 NCBI Official Synonym Symbols   
HH16; SemD; COLL1; SEMA1; SEMAD; SEMAL; coll-1; Hsema-I; SEMAIII; Hsema-III
[Similar Products]
 NCBI Protein Information    semaphorin-3A; collapsin 1; semaphorin D; semaphorin L; semaphorin III
 UniProt Protein Name    Semaphorin-3A
 UniProt Synonym Protein Names   
Semaphorin III; Sema III
 Protein Family    Semaphorin
 UniProt Gene Name    SEMA3A [Similar Products]
 UniProt Synonym Gene Names    SEMAD; Sema III [Similar Products]
 UniProt Entry Name    SEM3A_HUMAN
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 NCBI Summary for SEMA3A    This gene is a member of the semaphorin family and encodes a protein with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease. [provided by RefSeq, Jul 2008]
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 UniProt Comments for SEMA3A    SEMA3A: Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1. Belongs to the semaphorin family.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7p12.1

Cellular Component: membrane; axon; dendrite; extracellular region

Molecular Function: chemorepellent activity; receptor activity; semaphorin receptor binding

Biological Process: facial nerve structural organization; axon guidance; trigeminal nerve structural organization; regulation of heart rate; apoptosis; olfactory bulb development; dendrite morphogenesis; neuron migration; axonal fasciculation; negative chemotaxis; negative regulation of axon extension involved in axon guidance; axon extension involved in axon guidance; gonadotrophin-releasing hormone neuronal migration to the hypothalamus; sympathetic nervous system development; branchiomotor neuron axon guidance; regulation of axon extension involved in axon guidance; nerve development; sensory system development

Disease: Hypogonadotropic Hypogonadism 16 With Or Without Anosmia
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 Research Articles on SEMA3A    1. Biallelic SEMA3A defects cause a novel type of syndromic short stature.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with SEMA3A elisa kitPathways associated with SEMA3A elisa kit
 Reference Product  PubMed Publications
 NRP1 elisa kit  >71 publications with SEMA3A and NRP1
 PLXNA1 elisa kit  >34 publications with SEMA3A and PLXNA1
 NRP2 elisa kit  >23 publications with SEMA3A and NRP2
 PLXNA3 elisa kit  >17 publications with SEMA3A and PLXNA3
 RAC1 elisa kit  >16 publications with SEMA3A and RAC1
 PLXNA4 elisa kit  >16 publications with SEMA3A and PLXNA4
 CRMP1 elisa kit  >15 publications with SEMA3A and CRMP1
 CDK5 elisa kit  >13 publications with SEMA3A and CDK5
 FARP2 elisa kit  >2 publications with SEMA3A and FARP2
 DPYSL2 elisa kit  >1 publications with SEMA3A and DPYSL2
 Products by Pathway  Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 CRMPs In Sema3A Signaling Pathway antibodies  CRMPs In Sema3A Signaling Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 SEMA3A-Plexin Repulsion Signaling By Inhibiting Integrin Adhesion Pathway antibodies  SEMA3A-Plexin Repulsion Signaling By Inhibiting Integrin Adhesion Pathway Diagram
 Sema3A PAK Dependent Axon Repulsion Pathway antibodies  Sema3A PAK Dependent Axon Repulsion Pathway Diagram
 Semaphorin Interactions Pathway antibodies  Semaphorin Interactions Pathway Diagram
Diseases associated with SEMA3A elisa kitOrgans/Tissues associated with SEMA3A elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >90 publications with SEMA3A and Nervous System Diseases
 Brain Diseases Antibodies  >43 publications with SEMA3A and Brain Diseases
 Inflammation Antibodies  >19 publications with SEMA3A and Inflammation
 Pain Antibodies  >16 publications with SEMA3A and Pain
 Heart Diseases Antibodies  >13 publications with SEMA3A and Heart Diseases
 Neoplasm Metastasis Antibodies  >11 publications with SEMA3A and Neoplasm Metastasis
 Kidney Diseases Antibodies  >10 publications with SEMA3A and Kidney Diseases
 Adenocarcinoma Antibodies  >10 publications with SEMA3A and Adenocarcinoma
 Necrosis Antibodies  >9 publications with SEMA3A and Necrosis
 Neoplasms, Experimental Antibodies  >9 publications with SEMA3A and Neoplasms, Experimental
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >290 publications with SEMA3A and Brain
 Embryonic Tissue Antibodies  >150 publications with SEMA3A and Embryonic Tissue
 Blood Antibodies  >108 publications with SEMA3A and Blood
 Eye Antibodies  >66 publications with SEMA3A and Eye
 Muscle Antibodies  >55 publications with SEMA3A and Muscle
 Heart Antibodies  >36 publications with SEMA3A and Heart
 Lung Antibodies  >30 publications with SEMA3A and Lung
 Skin Antibodies  >25 publications with SEMA3A and Skin
 Connective Tissue Antibodies  >16 publications with SEMA3A and Connective Tissue
 Bone Marrow Antibodies  >13 publications with SEMA3A and Bone Marrow
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