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Sgk2 elisa kit :: Mouse Serine/threonine-protein kinase Sgk2 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2886412
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $380 +1 FREE 8GB USB
96-Strip-Wells  /  $500 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,170 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,840 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Serine/threonine-protein kinase Sgk2, ELISA Kit

★Popular Item★
 Also Known As   

Mouse Serine/threonine-protein kinase Sgk2 ELISA Kit

 Product Synonym Names    Serine/threonine-protein kinase Sgk2; Serum/glucocorticoid-regulated kinase 2; Sgk2; 2.7.11.1
 Product Gene Name   

Sgk2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2886412 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q9QZS5
 Species Reactivity    Mouse
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 Specificity    Recombinant and natural Mouse Serine/threonine-protein kinase Sgk2
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 Assay Type    Competitive
 Detection Range    0.78-50 ng/mL
 Sensitivity    0.415 ng/mL
 Intra-Assay CV    <=5.4%
 Inter-Assay CV    <=7.1%
 Recovery    80%
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 Preparation and Storage    For long term storage, please store the entire kit at -20 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Sgk2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Sgk2 purchase    MBS2886412 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Serine/threonine-protein kinase Sgk2, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.78-50 ng/mL in biological research samples containing Sgk2, with an estimated sensitivity of 0.415 ng/mL. The ELISA analytical biochemical technique of the MBS2886412 kit is based on Sgk2 antibody-Sgk2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Sgk2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Sgk2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for Sgk2 elisa kit

   Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen, During the reaction, target antigen in the sample or standard competes with a fixed amount of biotin-labeled target antigen for sites on a pre-coated antibody specific to target antigen. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of Sgk2 elisa kit    Sgk2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2886412. Click to request current manual
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NCBI/Uniprot data below describe general gene information for Sgk2. It may not necessarily be applicable to this product.
 NCBI GI #    599045044
 NCBI GeneID    27219
 NCBI Accession #    NP_001278081.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001291152.1 [Other Products]
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 UniProt Primary Accession #    Q9QZS5 [Other Products]
 UniProt Secondary Accession #    Q8R0P6; B7ZC31 [Other Products]
 UniProt Related Accession #    Q9QZS5 [Other Products]
 Molecular Weight    38,248 Da
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 NCBI Official Full Name    serine/threonine-protein kinase Sgk2 isoform b
 NCBI Official Synonym Full Names    serum/glucocorticoid regulated kinase 2
 NCBI Official Symbol    Sgk2 [Similar Products]
 NCBI Official Synonym Symbols   
Sgkl; AI098171; AW146006
[Similar Products]
 NCBI Protein Information    serine/threonine-protein kinase Sgk2
 UniProt Protein Name    Serine/threonine-protein kinase Sgk2
 UniProt Synonym Protein Names   
Serum/glucocorticoid-regulated kinase 2
 Protein Family    Serine/threonine-protein kinase
 UniProt Gene Name    Sgk2 [Similar Products]
 UniProt Entry Name    SGK2_MOUSE
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 UniProt Comments for Sgk2    SGK2: Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cell growth, survival and proliferation. Up- regulates Na(+) channels: SCNN1A/ENAC, K(+) channels: KCNA3/Kv1.3, KCNE1 and KCNQ1, amino acid transporter: SLC6A19, glutamate transporter: SLC1A6/EAAT4, glutamate receptors: GRIA1/GLUR1 and GRIK2/GLUR6, Na(+)/H(+) exchanger: SLC9A3/NHE3, and the Na(+)/K(+) ATPase. Highly expressed in liver, kidney and pancreas, and at lower levels in brain. Two specific sites, one in the kinase domain (Thr-253) and the other in the C-terminal regulatory region (Ser- 416), need to be phsphorylated for its full activation. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, AGC; EC 2.7.11.1; AGC group; SGK family

Cellular Component: cytoplasm; intracellular; nucleus

Molecular Function: ATP binding; calcium channel regulator activity; chloride channel regulator activity; kinase activity; nucleotide binding; potassium channel regulator activity; protein kinase activity; protein serine/threonine kinase activity; sodium channel regulator activity; transferase activity

Biological Process: cellular sodium ion homeostasis; peptidyl-serine phosphorylation; phosphorylation; positive regulation of transporter activity; protein amino acid phosphorylation; regulation of apoptosis; regulation of cell growth; regulation of cell proliferation
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 Research Articles on Sgk2    1. the nucleotide substitution in the Sgkl gene is the causative mutation for defective hair growth in the ypc mutant mouse and the signaling pathway involving SGKL plays an essential role in mammalian hair development
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Sgk2 elisa kitPathways associated with Sgk2 elisa kit
 Reference Product  PubMed Publications
 Foxo4 elisa kit  >1 publications with Sgk2 and Foxo4
 Pdpk1 elisa kit  >1 publications with Sgk2 and Pdpk1
 Products by Pathway  Pathway Diagram
 FoxO Signaling Pathway antibodies  FoxO Signaling Pathway Diagram
 Insulin Signaling Pathway antibodies  Insulin Signaling Pathway Diagram
 Ion Channel Transport Pathway antibodies  Ion Channel Transport Pathway Diagram
 PI3K-Akt Signaling Pathway antibodies  PI3K-Akt Signaling Pathway Diagram
 PI3K-Akt Signaling Pathway antibodies  PI3K-Akt Signaling Pathway Diagram
 Stimuli-sensing Channels Pathway antibodies  Stimuli-sensing Channels Pathway Diagram
 Transmembrane Transport Of Small Molecules Pathway antibodies  Transmembrane Transport Of Small Molecules Pathway Diagram
Diseases associated with Sgk2 elisa kitOrgans/Tissues associated with Sgk2 elisa kit
 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >3 publications with Sgk2 and Kidney Diseases
 Cardiovascular Diseases Antibodies  >2 publications with Sgk2 and Cardiovascular Diseases
 Weight Loss Antibodies  >2 publications with Sgk2 and Weight Loss
 Lung Neoplasms Antibodies  >1 publications with Sgk2 and Lung Neoplasms
 Hypertension Antibodies  >1 publications with Sgk2 and Hypertension
 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >15 publications with Sgk2 and Kidney
 Brain Antibodies  >6 publications with Sgk2 and Brain
 Liver Antibodies  >4 publications with Sgk2 and Liver
 Intestine Antibodies  >4 publications with Sgk2 and Intestine
 Pancreas Antibodies  >1 publications with Sgk2 and Pancreas
 Lung Antibodies  >1 publications with Sgk2 and Lung
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