Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow STAT3 rightarrow Mouse STAT3  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • glutamic-oxaloacetic transaminase 1, soluble (aspartate amin... ELISA Kit
 • WAF1, p21 Antibody
 • TKT Antibody
 • MAP-2 (2a + 2b) Microtubule Associated Protein 2 (MAP2) Antibody
 • Smad4 Antibody
 • CYP2E1 ELISA Kit
 • 1,4-alpha-glucan-branching enzyme (Gbe1) Recombinant Protein
 • Activin Receptor Type IIA (Acvr2a) Antibody
 • tartrate resistant acid phosphatase 5b (TRACP-5b) ELISA Kit
 • INS ELISA Kit
 • Interleukin-8, IL-8 (IL8-CXCL8) ELISA Kit
 • CCDC106 Antibody
 • Protoporphyrinogen oxidase (PPOX) Recombinant Protein
 • Indoleamine 2, 3 Dioxygenase (IDO) ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

STAT3 elisa kit :: Mouse Signal Transducer And Activator Of Transcription 3 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS726159
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Signal Transducer And Activator Of Transcription 3 (STAT3), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Signal Transducer And Activator Of Transcription 3 ELISA Kit

 Product Gene Name   

STAT3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Mouse
Section Bar
Section Bar
 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Sandwich
 Detection Range    1.0-25ng/mL
 Sensitivity    0.1ng/mL
Section Bar
 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of STAT3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for STAT3 purchase    MBS726159 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Signal Transducer And Activator Of Transcription 3 (STAT3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.0-25ng/mL in biological research samples containing STAT3, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS726159 kit is based on STAT3 antibody-STAT3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect STAT3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, STAT3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for STAT3 elisa kit

   Intended Uses: This STAT3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse STAT3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: STAT3 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for STAT3. Standards or samples are then added to the microtiter plate wells and STAT3 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of STAT3 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for STAT3 are added to each well to "sandwich" the STAT3 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain STAT3 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The STAT3 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for STAT3 elisa kit    Signal Transduction
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of STAT3 elisa kit    STAT3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS726159. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for STAT3. It may not necessarily be applicable to this product.
 NCBI GI #    458706
 NCBI GeneID    20848
 NCBI Accession #    AAA19452.1 [Other Products]
Section Bar
 UniProt Secondary Accession #    A2A5D1; B7ZC17 [Other Products]
 UniProt Related Accession #    P42227 [Other Products]
 Molecular Weight    87,967 Da
Table BarTOPTable Bar
 NCBI Official Full Name    Stat3
 NCBI Official Synonym Full Names    signal transducer and activator of transcription 3
 NCBI Official Symbol    Stat3 [Similar Products]
 NCBI Official Synonym Symbols   
Aprf; AW109958; 1110034C02Rik
[Similar Products]
 NCBI Protein Information    signal transducer and activator of transcription 3; acute phase response factor; acute-phase response factor
 UniProt Protein Name    Signal transducer and activator of transcription 3
 UniProt Synonym Protein Names   
Acute-phase response factor
 Protein Family    Signal transducer and activator of transcription
 UniProt Gene Name    Stat3 [Similar Products]
 UniProt Synonym Gene Names    Aprf [Similar Products]
 UniProt Entry Name    STAT3_MOUSE
Section Bar
 UniProt Comments for STAT3    STAT3: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of many cytokines and growth-factor receptors. Constitutively active in a number of human tumors. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described.

Protein type: Transcription factor; DNA-binding; Nuclear receptor co-regulator; Motility/polarity/chemotaxis

Cellular Component: nucleoplasm; mitochondrion; mitochondrial inner membrane; cytoplasm; plasma membrane; intracellular; nucleus

Molecular Function: protein dimerization activity; protein binding; ligand-dependent nuclear receptor activity; signal transducer activity; DNA binding; sequence-specific DNA binding; protein kinase binding; transcription factor activity; protein phosphatase binding; transcription factor binding; CCR5 chemokine receptor binding; glucocorticoid receptor binding

Biological Process: transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; radial glial cell differentiation; thermoregulation; glucose homeostasis; signal transduction; response to estradiol stimulus; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; astrocyte differentiation; protein import into nucleus; acute-phase response; negative regulation of glycolysis; positive regulation of Notch signaling pathway; intracellular receptor-mediated signaling pathway; transcription, DNA-dependent; eating behavior; cytokine and chemokine mediated signaling pathway; stem cell maintenance; regulation of multicellular organism growth; JAK-STAT cascade; cellular response to hormone stimulus; cell proliferation; sexual reproduction; response to cytokine stimulus; positive regulation of transcription from RNA polymerase II promoter; regulation of mitochondrial membrane permeability; eye photoreceptor cell differentiation; phosphorylation
Table BarTOPTable Bar
 Research Articles on STAT3    1. STAT3 was a substrate for SHP-1 by co-immunoprecipitation.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Pathways associated with STAT3 elisa kitDiseases associated with STAT3 elisa kit
 Products by Pathway  Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >1747 publications with STAT3 and Inflammation
 Carcinoma Antibodies  >1488 publications with STAT3 and Carcinoma
 Necrosis Antibodies  >794 publications with STAT3 and Necrosis
 Adenocarcinoma Antibodies  >662 publications with STAT3 and Adenocarcinoma
 Breast Neoplasms Antibodies  >479 publications with STAT3 and Breast Neoplasms
 Neoplasms, Experimental Antibodies  >465 publications with STAT3 and Neoplasms, Experimental
 Autoimmune Diseases Antibodies  >394 publications with STAT3 and Autoimmune Diseases
 Liver Neoplasms Antibodies  >389 publications with STAT3 and Liver Neoplasms
 Lung Neoplasms Antibodies  >345 publications with STAT3 and Lung Neoplasms
 Neoplasm Metastasis Antibodies  >314 publications with STAT3 and Neoplasm Metastasis
Organs/Tissues associated with STAT3 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >2686 publications with STAT3 and Blood
 Other Antibodies  >1675 publications with STAT3 and Other
 Liver Antibodies  >1284 publications with STAT3 and Liver
 Brain Antibodies  >1052 publications with STAT3 and Brain
 Muscle Antibodies  >781 publications with STAT3 and Muscle
 Lung Antibodies  >781 publications with STAT3 and Lung
 Bone Antibodies  >728 publications with STAT3 and Bone
 Heart Antibodies  >567 publications with STAT3 and Heart
 Skin Antibodies  >491 publications with STAT3 and Skin
 Bone Marrow Antibodies  >483 publications with STAT3 and Bone Marrow
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions