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Smpd1 elisa kit :: Mouse Sphingomyelin phosphodiesterase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2881002
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $380 +1 FREE 8GB USB
96-Strip-Wells  /  $500 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,170 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,840 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Sphingomyelin phosphodiesterase (Smpd1), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Sphingomyelin phosphodiesterase ELISA Kit

 Product Synonym Names    Sphingomyelin phosphodiesterase; Acid sphingomyelinase; aSMase; Smpd1; Asm; 3.1.4.12
 Product Gene Name   

Smpd1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2881002 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q04519
 Species Reactivity    Mouse
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 Assay Type    Sandwich
 Detection Range    0.312-20 ng/ml
 Sensitivity    0.156 ng/mL
 Intra-Assay CV    <=6.9%
 Inter-Assay CV    <=8.8%
 Recovery    86%
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 Preparation and Storage    For long term storage, please store the entire kit at -20 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Smpd1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Smpd1 purchase    MBS2881002 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Sphingomyelin phosphodiesterase (Smpd1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312-20 ng/ml in biological research samples containing Smpd1, with an estimated sensitivity of 0.156 ng/mL. The ELISA analytical biochemical technique of the MBS2881002 kit is based on Smpd1 antibody-Smpd1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Smpd1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Smpd1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for Smpd1 elisa kit

   Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Standard Curve/Testing Data of Smpd1 elisa kit    Smpd1 elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS2881002. Click to request current manual
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NCBI/Uniprot data below describe general gene information for Smpd1. It may not necessarily be applicable to this product.
 NCBI GI #    6755582
 NCBI GeneID    20597
 NCBI Accession #    NP_035551.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_011421.2 [Other Products]
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 UniProt Primary Accession #    Q04519 [Other Products]
 UniProt Secondary Accession #    Q3UL52 [Other Products]
 UniProt Related Accession #    Q04519 [Other Products]
 Molecular Weight    69,927 Da
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 NCBI Official Full Name    sphingomyelin phosphodiesterase
 NCBI Official Synonym Full Names    sphingomyelin phosphodiesterase 1, acid lysosomal
 NCBI Official Symbol    Smpd1 [Similar Products]
 NCBI Official Synonym Symbols   
ASM; aSMase; A-SMase; Zn-SMase
[Similar Products]
 NCBI Protein Information    sphingomyelin phosphodiesterase
 UniProt Protein Name    Sphingomyelin phosphodiesterase
 UniProt Synonym Protein Names   
Acid sphingomyelinase; aSMase
 Protein Family    Sphingomyelin phosphodiesterase
 UniProt Gene Name    Smpd1 [Similar Products]
 UniProt Synonym Gene Names    Asm; aSMase [Similar Products]
 UniProt Entry Name    ASM_MOUSE
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 UniProt Comments for Smpd1    SMPD1: Converts sphingomyelin to ceramide. Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol. Isoform 2 and isoform 3 have lost catalytic activity. Monomer. Belongs to the acid sphingomyelinase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.4.12; Phosphodiesterase; Lipid Metabolism - sphingolipid

Cellular Component: endosome; extracellular space; lamellar body; lysosome; plasma membrane

Molecular Function: hydrolase activity; hydrolase activity, acting on glycosyl bonds; sphingomyelin phosphodiesterase activity

Biological Process: ceramide biosynthetic process; metabolic process; negative regulation of MAP kinase activity; positive regulation of apoptosis; positive regulation of protein amino acid dephosphorylation; response to cocaine; sphingomyelin catabolic process
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 Research Articles on Smpd1    1. Intravenous injection of B16F10 melanoma cells into wild-type mice resulted in multiple lung metastases, while Asm-deficient mice (Smpd1(-/-) mice) were protected from pulmonary tumor spread.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Smpd1 elisa kitPathways associated with Smpd1 elisa kit
 Reference Product  PubMed Publications
 Asah1 elisa kit  >64 publications with Smpd1 and Asah1
 Asah2 elisa kit  >53 publications with Smpd1 and Asah2
 Gba elisa kit  >7 publications with Smpd1 and Gba
 Smpd2 elisa kit  >3 publications with Smpd1 and Smpd2
 Smpd4 elisa kit  >1 publications with Smpd1 and Smpd4
 Gba2 elisa kit  >1 publications with Smpd1 and Gba2
 Lass5 elisa kit  >1 publications with Smpd1 and Lass5
 Products by Pathway  Pathway Diagram
 Glycosphingolipid Metabolism Pathway antibodies  Glycosphingolipid Metabolism Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Ovarian Infertility Genes Pathway antibodies  Ovarian Infertility Genes Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram
Diseases associated with Smpd1 elisa kitOrgans/Tissues associated with Smpd1 elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >481 publications with Smpd1 and Nervous System Diseases
 Brain Diseases Antibodies  >454 publications with Smpd1 and Brain Diseases
 Niemann-Pick Diseases Antibodies  >386 publications with Smpd1 and Niemann-Pick Diseases
 Necrosis Antibodies  >320 publications with Smpd1 and Necrosis
 Disease Models, Animal Antibodies  >148 publications with Smpd1 and Disease Models, Animal
 Inflammation Antibodies  >147 publications with Smpd1 and Inflammation
 Liver Diseases Antibodies  >135 publications with Smpd1 and Liver Diseases
 Cardiovascular Diseases Antibodies  >116 publications with Smpd1 and Cardiovascular Diseases
 Niemann-Pick Disease, Type B Antibodies  >42 publications with Smpd1 and Niemann-Pick Disease, Type B
 Fibrosis Antibodies  >39 publications with Smpd1 and Fibrosis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >542 publications with Smpd1 and Blood
 Brain Antibodies  >303 publications with Smpd1 and Brain
 Liver Antibodies  >297 publications with Smpd1 and Liver
 Skin Antibodies  >179 publications with Smpd1 and Skin
 Muscle Antibodies  >127 publications with Smpd1 and Muscle
 Lung Antibodies  >120 publications with Smpd1 and Lung
 Bone Antibodies  >115 publications with Smpd1 and Bone
 Kidney Antibodies  >103 publications with Smpd1 and Kidney
 Bone Marrow Antibodies  >91 publications with Smpd1 and Bone Marrow
 Heart Antibodies  >77 publications with Smpd1 and Heart
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