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Sphk1 elisa kit :: Mouse Sphingosine kinase 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS288761 Sphk1 elisa kit
Unit / Price
48-Strip-Wells  /  $380 +1 FREE 8GB USB
96-Strip-Wells  /  $500 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,170 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,840 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Sphingosine kinase 1 (Sphk1), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Sphingosine kinase 1 ELISA Kit

 Product Synonym Names    Sphingosine kinase 1; SK 1; SPK 1; Sphk1; 2.7.1.91
 Product Gene Name   

Sphk1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for Q8CI15
 Species Reactivity    Mouse
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 Assay Type    Sandwich
 Detection Range    31.2-2000 pg/ml
 Sensitivity    15.75 pg/mL
 Intra-Assay CV    <=5.9%
 Inter-Assay CV    <=8.3%
 Recovery    107%
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 Preparation and Storage    For long term storage, please store the entire kit at -20 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Sphk1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Sphk1 purchase    MBS288761 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Sphingosine kinase 1 (Sphk1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.2-2000 pg/ml in biological research samples containing Sphk1, with an estimated sensitivity of 15.75 pg/mL. The ELISA analytical biochemical technique of the MBS288761 kit is based on Sphk1 antibody-Sphk1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Sphk1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Sphk1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for Sphk1 elisa kit

   Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS288761. Click to request current manual
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NCBI/Uniprot data below describe general gene information for Sphk1. It may not necessarily be applicable to this product.
 NCBI GI #    289191342
 NCBI GeneID    20698
 NCBI Accession #    NP_001165943.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001172472.1 [Other Products]
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 UniProt Primary Accession #    Q8CI15 [Other Products]
 UniProt Secondary Accession #    O88886; Q3U2E3; Q91ZN3 [Other Products]
 UniProt Related Accession #    Q8CI15 [Other Products]
 Molecular Weight    42,344 Da
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 NCBI Official Full Name    sphingosine kinase 1 isoform 2
 NCBI Official Synonym Full Names    sphingosine kinase 1
 NCBI Official Symbol    Sphk1 [Similar Products]
 NCBI Official Synonym Symbols   
Sk1; Spk1; 1110006G24Rik
[Similar Products]
 NCBI Protein Information    sphingosine kinase 1
 UniProt Protein Name    Sphingosine kinase 1
 Protein Family    Sphingosine kinase
 UniProt Gene Name    Sphk1 [Similar Products]
 UniProt Synonym Gene Names    SK 1; SPK 1 [Similar Products]
 UniProt Entry Name    SPHK1_MOUSE
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 NCBI Summary for Sphk1    This gene encodes a kinase that phosphorylates sphingosine into sphingosine-1-phosphate, which is involved in cell differentiation, motility, and apoptosis. The encoded protein plays a role in maintaining cellular levels of sphingosine-1-phosphate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]
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 UniProt Comments for Sphk1    SPHK1: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. Interacts with ACY1. Binds to calmodulin. Interacts with SPHKAP. Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - sphingolipid; Kinase, lipid; EC 2.7.1.91

Cellular Component: axon; cytoplasm; cytosol; membrane; neuron projection; nucleus; plasma membrane; synaptic vesicle

Molecular Function: ATP binding; calmodulin binding; D-erythro-sphingosine kinase activity; DNA binding; kinase activity; magnesium ion binding; nucleotide binding; protein phosphatase 2A binding; sphinganine kinase activity; transferase activity

Biological Process: activation of NF-kappaB transcription factor; blood vessel development; brain development; calcium-mediated signaling; cyclooxygenase pathway; inflammatory response; lipid phosphorylation; negative regulation of apoptosis; phosphorylation; positive regulation of angiogenesis; positive regulation of cell growth; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of fibroblast proliferation; positive regulation of mitotic cell cycle; positive regulation of neurotransmitter secretion; positive regulation of NF-kappaB import into nucleus; positive regulation of protein amino acid phosphorylation; positive regulation of protein ubiquitination; positive regulation of smooth muscle contraction; regulation of interleukin-1 beta production; sphingosine biosynthetic process; sphingosine metabolic process
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 Research Articles on Sphk1    1. Inhibition of SK1 attenuates the second phase of transmembrane Calcium influx.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Sphk1 elisa kitPathways associated with Sphk1 elisa kit
 Reference Product  PubMed Publications
 Sgpl1 elisa kit  >8 publications with Sphk1 and Sgpl1
 Sgpp1 elisa kit  >7 publications with Sphk1 and Sgpp1
 Asah1 elisa kit  >5 publications with Sphk1 and Asah1
 Sgpp2 elisa kit  >4 publications with Sphk1 and Sgpp2
 Lass5 elisa kit  >1 publications with Sphk1 and Lass5
 Lass1 elisa kit  >1 publications with Sphk1 and Lass1
 Ppap2a elisa kit  >1 publications with Sphk1 and Ppap2a
 Products by Pathway  Pathway Diagram
 Association Of TriC/CCT With Target Proteins During Biosynthesis Pathway antibodies  Association Of TriC/CCT With Target Proteins During Biosynthesis Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Chaperonin-mediated Protein Folding Pathway antibodies  Chaperonin-mediated Protein Folding Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
Diseases associated with Sphk1 elisa kitOrgans/Tissues associated with Sphk1 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >80 publications with Sphk1 and Inflammation
 Disease Models, Animal Antibodies  >47 publications with Sphk1 and Disease Models, Animal
 Necrosis Antibodies  >45 publications with Sphk1 and Necrosis
 Fibrosis Antibodies  >30 publications with Sphk1 and Fibrosis
 Cardiovascular Diseases Antibodies  >29 publications with Sphk1 and Cardiovascular Diseases
 Lung Diseases Antibodies  >28 publications with Sphk1 and Lung Diseases
 Breast Neoplasms Antibodies  >22 publications with Sphk1 and Breast Neoplasms
 Leukemia Antibodies  >20 publications with Sphk1 and Leukemia
 Kidney Diseases Antibodies  >18 publications with Sphk1 and Kidney Diseases
 Liver Diseases Antibodies  >18 publications with Sphk1 and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >52 publications with Sphk1 and Muscle
 Lung Antibodies  >42 publications with Sphk1 and Lung
 Brain Antibodies  >37 publications with Sphk1 and Brain
 Liver Antibodies  >34 publications with Sphk1 and Liver
 Kidney Antibodies  >31 publications with Sphk1 and Kidney
 Bone Antibodies  >31 publications with Sphk1 and Bone
 Bone Marrow Antibodies  >20 publications with Sphk1 and Bone Marrow
 Skin Antibodies  >14 publications with Sphk1 and Skin
 Lymph Node Antibodies  >12 publications with Sphk1 and Lymph Node
 Uterus Antibodies  >11 publications with Sphk1 and Uterus
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