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Cxcl12 elisa kit :: Mouse Stromal cell derived factor 1/SDF-1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS175856
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
1x96 Wells  /  $410 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Stromal cell derived factor 1/SDF-1 (Cxcl12), ELISA Kit

★Popular Item★
 Also Known As   

Mouse SDF-1 ELISA Kit

 Product Gene Name   

Cxcl12 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: 6; NC_000072.6 (117168535..117181368). Location: 6 F1; 6 54.81 cM
 3D Structure    ModBase 3D Structure for H7BX38
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 Species Reactivity    Mouse
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 Specificity    Natural and recombinant mouse SDF-1
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 Samples    For quantitative detection of mouse SDF-1 in cell culture supernates, serum and plasma(heparin).
 Detection Range    62.5pg/ml-4000pg/ml
 Samples    For quantitative detection of mouse SDF-1 in cell culture supernates, serum and plasma(heparin).
 Detection Range    62.5pg/ml-4000pg/ml
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 Preparation and Storage    Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Cxcl12 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Cxcl12 purchase    MBS175856 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Stromal cell derived factor 1/SDF-1 (Cxcl12) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 62.5pg/ml-4000pg/ml in biological research samples containing Cxcl12. The ELISA analytical biochemical technique of the MBS175856 kit is based on Cxcl12 antibody-Cxcl12 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Cxcl12 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Cxcl12. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as For quantitative detection of mouse SDF-1 in cell culture supernates, serum and plasma(heparin). Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for Cxcl12 elisa kit

   Principle of the Assay: mouse SDF-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for SDF-1 has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SDF-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse SDF-1 amount of sample captured in plate.

Background: SDF-1 (stromal cell-derived factor-1) is small cytokine belonging to the chemokine family that is officially designated Chemokine (C-X-C motif) ligand 12 (CXCL12). This gene is located on chromosome 10q11.1.1 SDF-1 is produced in two forms, SDF-1alpha/CXCL12a and SDF-1beta/CXCL12b, by alternate splicing of the same gene.2 Chemokines are characterized by the presence of four conserved cysteines, which form two disulfide bonds. The CXCL12 proteins belong to the group of CXC chemokines, whose initial pair of cysteines are separated by one intervening amino acid. CXCL12 is strongly chemotactic for lymphocytes.3,4,5,6 CXCL12 was shown to be expressed in many tissues in mice (including brain, thymus, heart, lung, liver, kidney, spleen and bone marrow). CXCL12 is a highly efficacious lymphocyte chemoattractant. In addition, CXCL12 induces intracellular actin polymerization in lymphocytes.7 CXCL12 is a substrate for the matrix metalloproteinase-2, which cleaves an CXCL12 N-terminal tetrapeptide.8 The standard product used in this kit is recombinant SDF-1 with the molecular mass of 8Kda.
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 Typical Testing Data/Standard Curve (for reference only) of Cxcl12 elisa kit    Cxcl12 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS175856. Click to request current manual
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NCBI/Uniprot data below describe general gene information for Cxcl12. It may not necessarily be applicable to this product.
 NCBI GI #    60279262
 NCBI GeneID    20315
 NCBI Accession #    NP_001012495.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001012477.2 [Other Products]
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 UniProt Primary Accession #    H7BX38 [Other Products]
 UniProt Related Accession #    P40224 [Other Products]
 Molecular Weight    13,634 Da [Similar Products]
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 NCBI Official Full Name    stromal cell-derived factor 1 isoform gamma
 NCBI Official Synonym Full Names    chemokine (C-X-C motif) ligand 12
 NCBI Official Symbol    Cxcl12 [Similar Products]
 NCBI Official Synonym Symbols   
Pbsf; Sdf1; Tlsf; Tpar1; Scyb12
[Similar Products]
 NCBI Protein Information    stromal cell-derived factor 1; pre-B-cell growth-stimulating factor; thymic lymphoma cell-stimulating factor; 12-O-tetradecanoylphorbol 13-acetate repressed protein 1
 UniProt Protein Name    Stromal cell-derived factor 1
 Protein Family    Stromal cell-derived factor
 UniProt Gene Name    Cxcl12 [Similar Products]
 UniProt Entry Name    H7BX38_MOUSE
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Product References and Citations for Cxcl12 elisa kit

   1. Shirozu, M.; Nakano, T.; Inazawa, J.; Tashiro, K.; Tada, H.; Shinohara, T.; Honjo, T. : Structure and chromosomal localization of the human stromal cell-derived factor 1 (SDF1) gene. Genomics 28: 495-500, 1995.
2. De La Luz Sierra et al. Differential processing of stromal-derived factor-1alpha and beta explains functional diversity. Blood 103:2452-2459, 2004.
3. Bleul et al. A highly efficacious lymphocyte chemoattractant, stromal cell-derived factor 1 (SDF-1). J. Exp. Med. 184: 1101-1109, 1996.
4. Ara et al. Impaired colonization of the gonads by primordial germ cells in mice lacking a chemokine, stromal cell-derived factor-1 (SDF-1). Proc. Nat. Acad. Sci. 100: 5319-5323, 2003.
5. Askari et al. Effect of stromal-cell-derived factor 1 on stem-cell homing and tissue regeneration in ischaemic cardiomyopathy. Lancet 362: 697-703, 2003.
6. Ma et al. Impaired B-lymphopoiesis, myelopoiesis, and derailed cerebellar neuron migration in CXCR4- and SDF-1-deficient mice. Proc. Nat. Acad. Sci. 95: 9448-9453, 1998.
7. Bleul, C. C.; Fuhlbrigge, R. C.; Casasnovas, J. M.; Aiuti, A.; Springer, T. A. : A highly efficacious lymphocyte chemoattractant, stromal cell-derived factor 1 (SDF-1). J. Exp. Med. 184: 1101-1109, 1996.
8. McQuibban, G. A.; Butler, G. S.; Gong, J. H.; Bendall, L.; Power, C.; Clark-Lewis, I.; Overall, C. M. : Matrix metalloproteinase activity inactivates the CXC chemokine stromal cell-derived factor-1. J. Biol. Chem. 276: 43503-43508, 2001.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with Cxcl12 elisa kitDiseases associated with Cxcl12 elisa kit
 Products by Pathway  Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Chemokine Receptors Bind Chemokines Pathway antibodies  Chemokine Receptors Bind Chemokines Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >1275 publications with Cxcl12 and Neoplasms
 Cardiovascular Diseases Antibodies  >471 publications with Cxcl12 and Cardiovascular Diseases
 Inflammation Antibodies  >456 publications with Cxcl12 and Inflammation
 Neoplasm Metastasis Antibodies  >320 publications with Cxcl12 and Neoplasm Metastasis
 HIV Infections Antibodies  >256 publications with Cxcl12 and HIV Infections
 Breast Neoplasms Antibodies  >229 publications with Cxcl12 and Breast Neoplasms
 Heart Diseases Antibodies  >220 publications with Cxcl12 and Heart Diseases
 Neoplasm Invasiveness Antibodies  >185 publications with Cxcl12 and Neoplasm Invasiveness
 Necrosis Antibodies  >182 publications with Cxcl12 and Necrosis
 Myocardial Infarction Antibodies  >170 publications with Cxcl12 and Myocardial Infarction
Organs/Tissues associated with Cxcl12 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >1250 publications with Cxcl12 and Bone
 Bone Marrow Antibodies  >1158 publications with Cxcl12 and Bone Marrow
 Brain Antibodies  >408 publications with Cxcl12 and Brain
 Muscle Antibodies  >308 publications with Cxcl12 and Muscle
 Lung Antibodies  >269 publications with Cxcl12 and Lung
 Lymph Node Antibodies  >252 publications with Cxcl12 and Lymph Node
 Heart Antibodies  >247 publications with Cxcl12 and Heart
 Liver Antibodies  >226 publications with Cxcl12 and Liver
 Embryonic Tissue Antibodies  >185 publications with Cxcl12 and Embryonic Tissue
 Spleen Antibodies  >126 publications with Cxcl12 and Spleen
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