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TAF1 elisa kit :: Mouse TAF1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2505389
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

TAF1, ELISA Kit

★Popular Item★
 Also Known As   

Mouse TAF1 (TATA Box Binding Protein Associated Factor 1) ELISA Kit

 Product Gene Name   

TAF1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    313650
 3D Structure    ModBase 3D Structure for P21675
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 Species Reactivity    Mouse
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 Specificity    This kit recognizes natural and recombinant Mouse TAF1. No significant cross-reactivity or interference between Mouse TAF1 and analogues was observed.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    15.625-1000pg/mL
 Sensitivity    Min: 9.375pg/mL; Max: 1000pg/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TAF1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TAF1 purchase    MBS2505389 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the TAF1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.625-1000pg/mL in biological research samples containing TAF1, with an estimated sensitivity of Min: 9.375pg/mL; Max: 1000pg/mL. The ELISA analytical biochemical technique of the MBS2505389 kit is based on TAF1 antibody-TAF1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TAF1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TAF1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for TAF1 elisa kit

   Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse TAF1 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TAF1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TAF1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TAF1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TAF1. You can calculate the concentration of TAF1 in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of TAF1 elisa kit    TAF1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2505389. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TAF1. It may not necessarily be applicable to this product.
 NCBI GI #    119625700
 NCBI GeneID    6872
 NCBI Accession #    EAX05295.1 [Other Products]
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 UniProt Primary Accession #    P21675 [Other Products]
 UniProt Secondary Accession #    Q59FZ3; Q6IUZ1; Q70Q86; Q70Q87; Q70T00; Q70T01; A5CVC8; A5CVC9; A5CVD0; A5CVD1; B1Q2X3 [Other Products]
 UniProt Related Accession #    P21675 [Other Products]
 Molecular Weight    214,913 Da
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 NCBI Official Full Name    TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa, isoform CRA_e
 NCBI Official Synonym Full Names    TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa
 NCBI Official Symbol    TAF1 [Similar Products]
 NCBI Official Synonym Symbols   
OF; XDP; BA2R; CCG1; CCGS; DYT3; KAT4; P250; NSCL2; TAF2A; N-TAF1; TAFII250; DYT3/TAF1; TAFII-250; TAF(II)250
[Similar Products]
 NCBI Protein Information    transcription initiation factor TFIID subunit 1; TBP-associated factor 250 kDa; cell cycle gene 1 protein; cell cycle, G1 phase defect; complementation of cell cycle block, G1-to-S; transcription factor TFIID p250 polypeptide
 UniProt Protein Name    Transcription initiation factor TFIID subunit 1
 UniProt Synonym Protein Names   
Cell cycle gene 1 protein; TBP-associated factor 250 kDa; p250; Transcription initiation factor TFIID 250 kDa subunit; TAF(II)250; TAFII-250; TAFII250
 Protein Family    Taz1-interacting factor
 UniProt Gene Name    TAF1 [Similar Products]
 UniProt Synonym Gene Names    BA2R; CCG1; CCGS; TAF2A; p250; TAF(II)250; TAFII-250; TAFII250 [Similar Products]
 UniProt Entry Name    TAF1_HUMAN
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 NCBI Summary for TAF1    Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N- and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conjugating enzyme. Mutations in this gene result in Dystonia 3, torsion, X-linked, a dystonia-parkinsonism disorder. Alternative splicing of this gene results in multiple transcript variants. This gene is part of a complex transcription unit (TAF1/DYT3), wherein some transcript variants share exons with TAF1 as well as additional downstream DYT3 exons. [provided by RefSeq, Oct 2013]
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 UniProt Comments for TAF1    TAF1: Largest component and core scaffold of the TFIID basal transcription factor complex. Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA- binding activity. Essential for progression of the G1 phase of the cell cycle. Defects in TAF1 are the cause of dystonia type 3 (DYT3); also called X-linked dystonia-parkinsonism (XDP). DYT3 is a X-linked dystonia-parkinsonism disorder. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT3 is characterized by severe progressive torsion dystonia followed by parkinsonism. Its prevalence is high in the Philippines. DYT3 has a well-defined pathology of extensive neuronal loss and mosaic gliosis in the striatum (caudate nucleus and putamen) which appears to resemble that in Huntington disease. Belongs to the TAF1 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; EC 2.3.1.48; Transcription, coactivator/corepressor; DNA-binding; Protein kinase, atypical; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); ATYPICAL group; TAF1 family

Chromosomal Location of Human Ortholog: Xq13.1

Cellular Component: nucleoplasm; transcription factor TFIID complex

Molecular Function: protein serine/threonine kinase activity; protein binding; histone acetyltransferase activity; sequence-specific DNA binding; TATA-binding protein binding; p53 binding; transcription coactivator activity; transcription factor binding; ATP binding

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; viral reproduction; protein amino acid autophosphorylation; transcription initiation; peptidyl-threonine phosphorylation; transcriptional preinitiation complex assembly; cell cycle; peptidyl-serine phosphorylation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; RNA elongation from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; gene expression; histone acetylation; response to DNA damage stimulus

Disease: Dystonia 3, Torsion, X-linked
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 Research Articles on TAF1    1. the surface of the TAF1-TAF7 complex contains two prominent conserved surface pockets, one of which binds selectively to an inhibitory trimethylated histone H3
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with TAF1 elisa kitDiseases associated with TAF1 elisa kit
 Products by Pathway  Pathway Diagram
 Basal Transcription Factors Pathway antibodies  Basal Transcription Factors Pathway Diagram
 Basal Transcription Factors Pathway antibodies  Basal Transcription Factors Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 HIV Infection Pathway antibodies  HIV Infection Pathway Diagram
 HIV Life Cycle Pathway antibodies  HIV Life Cycle Pathway Diagram
 HIV Transcription Initiation Pathway antibodies  HIV Transcription Initiation Pathway Diagram
 Late Phase Of HIV Life Cycle Pathway antibodies  Late Phase Of HIV Life Cycle Pathway Diagram
 RNA Polymerase II HIV Promoter Escape Pathway antibodies  RNA Polymerase II HIV Promoter Escape Pathway Diagram
 RNA Polymerase II Pre-transcription Events Pathway antibodies  RNA Polymerase II Pre-transcription Events Pathway Diagram
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >20 publications with TAF1 and Nervous System Diseases
 Heart Diseases Antibodies  >16 publications with TAF1 and Heart Diseases
 Brain Diseases Antibodies  >15 publications with TAF1 and Brain Diseases
 Disease Models, Animal Antibodies  >4 publications with TAF1 and Disease Models, Animal
 Atrophy Antibodies  >3 publications with TAF1 and Atrophy
 Liver Diseases Antibodies  >2 publications with TAF1 and Liver Diseases
 Genital Diseases, Male Antibodies  >2 publications with TAF1 and Genital Diseases, Male
 Liver Neoplasms Antibodies  >1 publications with TAF1 and Liver Neoplasms
 Infertility, Male Antibodies  >1 publications with TAF1 and Infertility, Male
 Carcinoma, Hepatocellular Antibodies  >1 publications with TAF1 and Carcinoma, Hepatocellular
Organs/Tissues associated with TAF1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >21 publications with TAF1 and Brain
 Heart Antibodies  >18 publications with TAF1 and Heart
 Muscle Antibodies  >7 publications with TAF1 and Muscle
 Blood Antibodies  >6 publications with TAF1 and Blood
 Testis Antibodies  >4 publications with TAF1 and Testis
 Lung Antibodies  >2 publications with TAF1 and Lung
 Kidney Antibodies  >2 publications with TAF1 and Kidney
 Bone Antibodies  >2 publications with TAF1 and Bone
 Ear Antibodies  >2 publications with TAF1 and Ear
 Liver Antibodies  >2 publications with TAF1 and Liver
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