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TAF4 elisa kit :: Mouse TAF4 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2510144
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

TAF4, ELISA Kit

★Popular Item★
 Also Known As   

Mouse TAF4 (TATA Box Binding Protein Associated Factor 4) ELISA Kit

 Product Gene Name   

TAF4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    601796
 3D Structure    ModBase 3D Structure for O00268
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 Species Reactivity    Mouse
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 Specificity    This kit recognizes natural and recombinant Mouse TAF4. No significant cross-reactivity or interference between Mouse TAF4 and analogues was observed.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    15.625-1000pg/mL
 Sensitivity    Min: 9.375pg/mL; Max: 1000pg/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TAF4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TAF4 purchase    MBS2510144 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the TAF4, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.625-1000pg/mL in biological research samples containing TAF4, with an estimated sensitivity of Min: 9.375pg/mL; Max: 1000pg/mL. The ELISA analytical biochemical technique of the MBS2510144 kit is based on TAF4 antibody-TAF4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TAF4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TAF4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for TAF4 elisa kit

   Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse TAF4 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TAF4. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TAF4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TAF4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TAF4. You can calculate the concentration of TAF4 in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of TAF4 elisa kit    TAF4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2510144. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TAF4. It may not necessarily be applicable to this product.
 NCBI GI #    110832843
 NCBI GeneID    6874
 NCBI Accession #    NP_003176.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_003185.3 [Other Products]
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 UniProt Primary Accession #    O00268 [Other Products]
 UniProt Secondary Accession #    Q5TBP6; Q99721; Q9BR40; Q9BX42; A6NGD9 [Other Products]
 UniProt Related Accession #    O00268 [Other Products]
 Molecular Weight    110,114 Da
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 NCBI Official Full Name    transcription initiation factor TFIID subunit 4
 NCBI Official Synonym Full Names    TAF4 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 135kDa
 NCBI Official Symbol    TAF4 [Similar Products]
 NCBI Official Synonym Symbols   
TAF2C; TAF4A; TAF2C1; TAFII130; TAFII135
[Similar Products]
 NCBI Protein Information    transcription initiation factor TFIID subunit 4; RNA polymerase II TBP-associated factor subunit C; TAF(II)130; TAF(II)135; TAF4A RNA polymerase II, TATA box binding protein (TBP)-associated factor, 135 kD; TAFII-130; TAFII-135; TATA box binding protein (TBP)-associated factor, RNA polymerase II, C1, 130kD; TBP-associated factor 4; transcription initiation factor TFIID 130 kDa subunit; transcription initiation factor TFIID 135 kD subunit; transcription initiation factor TFIID 135 kDa subunit
 UniProt Protein Name    Transcription initiation factor TFIID subunit 4
 UniProt Synonym Protein Names   
RNA polymerase II TBP-associated factor subunit C; TBP-associated factor 4; Transcription initiation factor TFIID 130 kDa subunit; TAF(II)130; TAFII-130; TAFII130; Transcription initiation factor TFIID 135 kDa subunit; TAF(II)135; TAFII-135; TAFII135
 Protein Family    Transcription initiation factor
 UniProt Gene Name    TAF4 [Similar Products]
 UniProt Synonym Gene Names    TAF2C; TAF2C1; TAF4A; TAFII130; TAFII135; TAF(II)130; TAFII-130; TAFII130; TAF(II)135; TAFII-135; TAFII135 [Similar Products]
 UniProt Entry Name    TAF4_HUMAN
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 NCBI Summary for TAF4    Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the larger subunits of TFIID that has been shown to potentiate transcriptional activation by retinoic acid, thyroid hormone and vitamin D3 receptors. In addition, this subunit interacts with the transcription factor CREB, which has a glutamine-rich activation domain, and binds to other proteins containing glutamine-rich regions. Aberrant binding to this subunit by proteins with expanded polyglutamine regions has been suggested as one of the pathogenetic mechanisms underlying a group of neurodegenerative disorders referred to as polyglutamine diseases. [provided by RefSeq, Jul 2008]
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 UniProt Comments for TAF4    TAF4: Makes part of TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Potentiates transcriptional activation by the AF-2S of the retinoic acid, vitamin D3 and thyroid hormone. Belongs to the TAF4 family.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 20q13.33

Cellular Component: nucleoplasm; transcription factor TFIID complex; cytoplasm

Molecular Function: protein binding; DNA binding; protein heterodimerization activity; transcription coactivator activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; viral reproduction; ovarian follicle development; positive regulation of transcription, DNA-dependent; RNA elongation from RNA polymerase II promoter; transcription initiation; gene expression
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 Research Articles on TAF4    1. TAF4 isoforms generated by the alternative splicing participate in the conversion of the cellular transcriptional programs from the maintenance of stem cell state to differentiation
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TAF4 elisa kitPathways associated with TAF4 elisa kit
 Reference Product  PubMed Publications
 TBP elisa kit  >32 publications with TAF4 and TBP
 TAF5 elisa kit  >9 publications with TAF4 and TAF5
 TAF12 elisa kit  >9 publications with TAF4 and TAF12
 TAF10 elisa kit  >4 publications with TAF4 and TAF10
 TAF2 elisa kit  >3 publications with TAF4 and TAF2
 TAF7 elisa kit  >3 publications with TAF4 and TAF7
 TAF8 elisa kit  >1 publications with TAF4 and TAF8
 TAF11 elisa kit  >1 publications with TAF4 and TAF11
 Products by Pathway  Pathway Diagram
 Basal Transcription Factors Pathway antibodies  Basal Transcription Factors Pathway Diagram
 Basal Transcription Factors Pathway antibodies  Basal Transcription Factors Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 HIV Infection Pathway antibodies  HIV Infection Pathway Diagram
 HIV Life Cycle Pathway antibodies  HIV Life Cycle Pathway Diagram
 HIV Transcription Initiation Pathway antibodies  HIV Transcription Initiation Pathway Diagram
 Herpes Simplex Infection Pathway antibodies  Herpes Simplex Infection Pathway Diagram
 Herpes Simplex Infection Pathway antibodies  Herpes Simplex Infection Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram
Diseases associated with TAF4 elisa kitOrgans/Tissues associated with TAF4 elisa kit
 Disease Name  Pubmed Publications
 Death Antibodies  >4 publications with TAF4 and Death
 Atrophy Antibodies  >2 publications with TAF4 and Atrophy
 Chromosome Aberrations Antibodies  >2 publications with TAF4 and Chromosome Aberrations
 Skin Neoplasms Antibodies  >1 publications with TAF4 and Skin Neoplasms
 Nerve Degeneration Antibodies  >1 publications with TAF4 and Nerve Degeneration
 Hyperplasia Antibodies  >1 publications with TAF4 and Hyperplasia
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >11 publications with TAF4 and Brain
 Blood Antibodies  >3 publications with TAF4 and Blood
 Testis Antibodies  >3 publications with TAF4 and Testis
 Pancreas Antibodies  >1 publications with TAF4 and Pancreas
 Connective Tissue Antibodies  >1 publications with TAF4 and Connective Tissue
 Skin Antibodies  >1 publications with TAF4 and Skin
 Thymus Antibodies  >1 publications with TAF4 and Thymus
 Eye Antibodies  >1 publications with TAF4 and Eye
 Embryonic Tissue Antibodies  >1 publications with TAF4 and Embryonic Tissue
 Liver Antibodies  >1 publications with TAF4 and Liver
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