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TIMP-3 elisa kit :: Mouse TIMP-3 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2500590 (SPECIAL PROMOTIONAL PRICING for a limited time)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $310 +1 FREE 8GB USB
96-Strip-Wells  /  $355 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,435 +1 FREE 8GB USB
10x96-Strip-Wells  /  $2,805 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

TIMP-3, ELISA Kit

★Popular Item★
 Also Known As   

Mouse TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) ELISA Kit

 Product Gene Name   

TIMP-3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    136900
 3D Structure    ModBase 3D Structure for P35625
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 Species Reactivity    Mouse
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 Specificity    This kit recognizes natural and some recombinant Mouse TIMP-3. No Significant cross -reactivity or interference between Mouse TIMP-3and analogues was observed.
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 Samples    Serum, plasma and other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    0.16-10ng/mL
 Sensitivity    0.1ng/mL.
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TIMP-3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TIMP-3 purchase    MBS2500590 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the TIMP-3, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.16-10ng/mL in biological research samples containing TIMP-3, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS2500590 kit is based on TIMP-3 antibody-TIMP-3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TIMP-3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TIMP-3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for TIMP-3 elisa kit

   Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TIMP-3 in Mouse serum, plasma and other biological fluids

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TIMP-3. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse TIMP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TIMP-3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse TIMP-3. You can calculate the concentration of Mouse TIMP-3 in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of TIMP-3 elisa kit    TIMP-3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2500590. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TIMP-3. It may not necessarily be applicable to this product.
 NCBI GI #    998826
 NCBI GeneID    7078
 NCBI Accession #    AAB34532.1 [Other Products]
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 UniProt Primary Accession #    P35625 [Other Products]
 UniProt Secondary Accession #    Q5THV4; Q9UC74; Q9UGS2; B2RBY9 [Other Products]
 UniProt Related Accession #    P35625 [Other Products]
 Molecular Weight    24,145 Da
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 NCBI Official Full Name    TIMP-3
 NCBI Official Synonym Full Names    TIMP metallopeptidase inhibitor 3
 NCBI Official Symbol    TIMP3 [Similar Products]
 NCBI Official Synonym Symbols   
SFD; K222; K222TA2; HSMRK222
[Similar Products]
 NCBI Protein Information    metalloproteinase inhibitor 3; MIG-5 protein; TIMP-3; protein MIG-5; tissue inhibitor of metalloproteinases 3
 UniProt Protein Name    Metalloproteinase inhibitor 3
 UniProt Synonym Protein Names   
Protein MIG-5; Tissue inhibitor of metalloproteinases 3; TIMP-3
 UniProt Gene Name    TIMP3 [Similar Products]
 UniProt Synonym Gene Names    TIMP-3 [Similar Products]
 UniProt Entry Name    TIMP3_HUMAN
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 NCBI Summary for TIMP-3    This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM). Expression of this gene is induced in response to mitogenic stimulation and this netrin domain-containing protein is localized to the ECM. Mutations in this gene have been associated with the autosomal dominant disorder Sorsby's fundus dystrophy. [provided by RefSeq, Jul 2008]
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 UniProt Comments for TIMP-3    TIMP3: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15. Interacts with EFEMP1. Belongs to the protease inhibitor I35 (TIMP) family.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 22q12.3

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; basement membrane; nucleus

Molecular Function: metalloendopeptidase inhibitor activity; protein binding; protease binding; metal ion binding

Biological Process: negative regulation of metalloenzyme activity; visual perception; response to cytokine stimulus; response to hormone stimulus; negative regulation of membrane protein ectodomain proteolysis

Disease: Fundus Dystrophy, Pseudoinflammatory, Of Sorsby
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 Research Articles on TIMP-3    1. Data show that gene polymorphisms of TIMP metallopeptidase inhibitors TIMP-3 -1296 T>C (rs9619311) and TIMP-4 -55 T>C (rs3755724) were associated with the susceptibility of hepatocellular carcinoma among Taiwan women.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TIMP-3 elisa kitPathways associated with TIMP-3 elisa kit
 Reference Product  PubMed Publications
 MMP2 elisa kit  >74 publications with TIMP-3 and MMP2
 MMP9 elisa kit  >65 publications with TIMP-3 and MMP9
 GSTP1 elisa kit  >52 publications with TIMP-3 and GSTP1
 MMP3 elisa kit  >37 publications with TIMP-3 and MMP3
 ADAM17 elisa kit  >21 publications with TIMP-3 and ADAM17
 TGFB1 elisa kit  >19 publications with TIMP-3 and TGFB1
 EFEMP1 elisa kit  >9 publications with TIMP-3 and EFEMP1
 TP53 elisa kit  >8 publications with TIMP-3 and TP53
 OSM elisa kit  >2 publications with TIMP-3 and OSM
 Products by Pathway  Pathway Diagram
 Angiogenesis Pathway antibodies  Angiogenesis Pathway Diagram
 Endochondral Ossification Pathway antibodies  Endochondral Ossification Pathway Diagram
 Matrix Metalloproteinases Pathway antibodies  Matrix Metalloproteinases Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram
 Oncostatin M Signaling Pathway antibodies  Oncostatin M Signaling Pathway Diagram
 Proteoglycans In Cancer Pathway antibodies  Proteoglycans In Cancer Pathway Diagram
 Proteoglycans In Cancer Pathway antibodies  Proteoglycans In Cancer Pathway Diagram
Diseases associated with TIMP-3 elisa kitOrgans/Tissues associated with TIMP-3 elisa kit
 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >73 publications with TIMP-3 and Adenocarcinoma
 Necrosis Antibodies  >64 publications with TIMP-3 and Necrosis
 Inflammation Antibodies  >60 publications with TIMP-3 and Inflammation
 Cardiovascular Diseases Antibodies  >50 publications with TIMP-3 and Cardiovascular Diseases
 Neoplasm Metastasis Antibodies  >45 publications with TIMP-3 and Neoplasm Metastasis
 Disease Models, Animal Antibodies  >37 publications with TIMP-3 and Disease Models, Animal
 Breast Neoplasms Antibodies  >36 publications with TIMP-3 and Breast Neoplasms
 Liver Diseases Antibodies  >31 publications with TIMP-3 and Liver Diseases
 Neoplasms, Experimental Antibodies  >29 publications with TIMP-3 and Neoplasms, Experimental
 Heart Diseases Antibodies  >26 publications with TIMP-3 and Heart Diseases
 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >74 publications with TIMP-3 and Vascular
 Blood Antibodies  >73 publications with TIMP-3 and Blood
 Eye Antibodies  >71 publications with TIMP-3 and Eye
 Liver Antibodies  >46 publications with TIMP-3 and Liver
 Lung Antibodies  >42 publications with TIMP-3 and Lung
 Muscle Antibodies  >34 publications with TIMP-3 and Muscle
 Brain Antibodies  >30 publications with TIMP-3 and Brain
 Heart Antibodies  >29 publications with TIMP-3 and Heart
 Embryonic Tissue Antibodies  >29 publications with TIMP-3 and Embryonic Tissue
 Bone Antibodies  >27 publications with TIMP-3 and Bone
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