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TPO elisa kit :: Mouse TPO ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2510249 (SPECIAL PROMOTIONAL PRICING for a limited time)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $310 +1 FREE 8GB USB
96-Strip-Wells  /  $355 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,435 +1 FREE 8GB USB
10x96-Strip-Wells  /  $2,805 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

TPO, ELISA Kit

★Popular Item★
 Also Known As   

Mouse TPO (Thrombopoietin) ELISA Kit

 Product Gene Name   

TPO elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2510249 COA    COA PDF
 OMIM    274500
 3D Structure    ModBase 3D Structure for P07202
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 Species Reactivity    Mouse
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 Specificity    This kit recognizes natural and recombinant Mouse TPO. No significant cross-reactivity or interference between Mouse TPO and analogues was observed.
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 Samples    Serum, plasma and other biological fluids.
 Assay Type    Sandwich
 Detection Range    7.813-500pg/mL
 Sensitivity    The minimum detectable dose of Mouse TPO is 4.688pg/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).
 Intended Uses    This ELISA kit applies to the in vitro quantitative determination of Mouse TPO concentrations in serum, plasma and other biological fluids.
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TPO elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TPO purchase    MBS2510249 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the TPO, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 7.813-500pg/mL in biological research samples containing TPO, with an estimated sensitivity of The minimum detectable dose of Mouse TPO is 4.688pg/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero). The ELISA analytical biochemical technique of the MBS2510249 kit is based on TPO antibody-TPO antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TPO antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TPO. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for TPO elisa kit

   Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TPO. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TPO and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TPO, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TPO. You can calculate the concentration of TPO in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of TPO elisa kit    TPO elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2510249. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TPO. It may not necessarily be applicable to this product.
 NCBI GI #    28558988
 NCBI GeneID    7173
 NCBI Accession #    NP_783652.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_175721.3 [Other Products]
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 UniProt Primary Accession #    P07202 [Other Products]
 UniProt Secondary Accession #    P09934; P09935; Q8IUL0; Q8NF94; Q8NF95; Q8NF96; Q8NF97; Q8TCI9 [Other Products]
 UniProt Related Accession #    P07202 [Other Products]
 Molecular Weight    92,063 Da
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 NCBI Official Full Name    thyroid peroxidase isoform d
 NCBI Official Synonym Full Names    thyroid peroxidase
 NCBI Official Symbol    TPO [Similar Products]
 NCBI Official Synonym Symbols   
MSA; TPX; TDH2A
[Similar Products]
 NCBI Protein Information    thyroid peroxidase; thyroid microsomal antigen; thyroperoxidase
 UniProt Protein Name    Thyroid peroxidase
 Protein Family    Thyroid peroxidase
 UniProt Gene Name    TPO [Similar Products]
 UniProt Synonym Gene Names    TPO [Similar Products]
 UniProt Entry Name    PERT_HUMAN
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 NCBI Summary for TPO    This gene encodes a membrane-bound glycoprotein. The encoded protein acts as an enzyme and plays a central role in thyroid gland function. The protein functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mutations in this gene are associated with several disorders of thyroid hormonogenesis, including congenital hypothyroidism, congenital goiter, and thyroid hormone organification defect IIA. Multiple transcript variants encoding distinct isoforms have been identified for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, May 2011]
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 UniProt Comments for TPO    TPO: Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4). An alternative splicing in the thyroperoxidase mRNA can cause Graves' disease. Defects in TPO are the cause of thyroid dyshormonogenesis 2A (TDH2A). A disorder due to defective conversion of accumulated iodide to organically bound iodine. The iodide organification defect can be partial or complete. Belongs to the peroxidase family. XPO subfamily. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Oxidoreductase; Membrane protein, integral; EC 1.11.1.8; Amino Acid Metabolism - tyrosine

Chromosomal Location of Human Ortholog: 2p25

Cellular Component: extracellular space; cell surface; mitochondrion; integral to plasma membrane; plasma membrane

Molecular Function: peroxidase activity; heme binding; iodide peroxidase activity; calcium ion binding

Biological Process: hydrogen peroxide catabolic process; embryonic hemopoiesis; thyroid hormone generation; response to oxidative stress; hormone biosynthetic process

Disease: Thyroid Dyshormonogenesis 2a
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 Research Articles on TPO    1. In conclusion, the c.2268dup mutation leads to the formation of normal and alternatively spliced TPO mRNA transcripts with a consequential loss of TPO enzymatic activity in Malaysian-Chinese patients with goitrous CH.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with TPO elisa kitDiseases associated with TPO elisa kit
 Products by Pathway  Pathway Diagram
 Amine-derived Hormones Pathway antibodies  Amine-derived Hormones Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Amino Acids And Derivatives Pathway antibodies  Metabolism Of Amino Acids And Derivatives Pathway Diagram
 Thyroid Hormone Biosynthesis, Tyrosine => Triiodothyronine/thyroxine Pathway antibodies  Thyroid Hormone Biosynthesis, Tyrosine => Triiodothyronine/thyroxine Pathway Diagram
 Thyroid Hormone Biosynthesis, Tyrosine => Triiodothyronine/thyroxine Pathway antibodies  Thyroid Hormone Biosynthesis, Tyrosine => Triiodothyronine/thyroxine Pathway Diagram
 Thyroid Hormone Synthesis Pathway antibodies  Thyroid Hormone Synthesis Pathway Diagram
 Thyroid Hormone Synthesis Pathway antibodies  Thyroid Hormone Synthesis Pathway Diagram
 Disease Name  Pubmed Publications
 Thyroid Diseases Antibodies  >1258 publications with TPO and Thyroid Diseases
 Goiter Antibodies  >477 publications with TPO and Goiter
 Thyroid Neoplasms Antibodies  >200 publications with TPO and Thyroid Neoplasms
 Blood Coagulation Disorders Antibodies  >136 publications with TPO and Blood Coagulation Disorders
 Liver Diseases Antibodies  >128 publications with TPO and Liver Diseases
 Congenital Hypothyroidism Antibodies  >108 publications with TPO and Congenital Hypothyroidism
 Hepatitis Antibodies  >94 publications with TPO and Hepatitis
 Mental Disorders Antibodies  >93 publications with TPO and Mental Disorders
 Central Nervous System Diseases Antibodies  >85 publications with TPO and Central Nervous System Diseases
 Brain Diseases Antibodies  >79 publications with TPO and Brain Diseases
Organs/Tissues associated with TPO elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >2191 publications with TPO and Blood
 Thyroid Antibodies  >1788 publications with TPO and Thyroid
 Brain Antibodies  >168 publications with TPO and Brain
 Spleen Antibodies  >124 publications with TPO and Spleen
 Muscle Antibodies  >62 publications with TPO and Muscle
 Heart Antibodies  >54 publications with TPO and Heart
 Connective Tissue Antibodies  >27 publications with TPO and Connective Tissue
 Adipose Tissue Antibodies  >11 publications with TPO and Adipose Tissue
 Intestine Antibodies  >10 publications with TPO and Intestine
 Testis Antibodies  >5 publications with TPO and Testis
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