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TRACP-5b elisa kit :: Mouse TRACP-5b ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2502462 TRACP-5b elisa kit
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

TRACP-5b, ELISA Kit

★Popular Item★
 Also Known As   

Mouse TRACP-5b (Tartrate-Resistant Acid Phosphatase 5b) ELISA Kit

 Product Gene Name   

TRACP-5b elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2502462 COA    COA PDF
 OMIM    171640
 3D Structure    ModBase 3D Structure for P13686
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 Species Reactivity    Mouse
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 Specificity    This kit recognizes natural and recombinantMouseTRACP-5b. No significant cross-reactivity or interference between MouseTRACP-5b and analogues was observed.
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 Samples    Serum, plasma and other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    0.781-50ng/mL
 Sensitivity    0.469ng/mL
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 Preparation and Storage    Store at 4 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TRACP-5b elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TRACP-5b purchase    MBS2502462 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the TRACP-5b, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.781-50ng/mL in biological research samples containing TRACP-5b, with an estimated sensitivity of 0.469ng/mL. The ELISA analytical biochemical technique of the MBS2502462 kit is based on TRACP-5b antibody-TRACP-5b antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TRACP-5b antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TRACP-5b. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for TRACP-5b elisa kit

   Intended Uses: This ELISA kit applies to the invitro quantitative determination of MouseTRACP-5bconcentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TRACP-5b. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TRACP-5band Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TRACP-5b, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TRACP-5b.You can calculate the concentration of TRACP-5bin the samples by comparing the OD of the samples to the standard curve.
Sample Manual Insert of MBS2502462. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TRACP-5b. It may not necessarily be applicable to this product.
 NCBI GI #    161377455
 NCBI GeneID    54
 NCBI Accession #    NP_001104506.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001111036.1 [Other Products]
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 UniProt Primary Accession #    P13686 [Other Products]
 UniProt Secondary Accession #    Q2TAB1; Q6IAS6; Q9UCJ5; Q9UCJ6; Q9UCJ7; A8K3V2 [Other Products]
 UniProt Related Accession #    P13686 [Other Products]
 Molecular Weight    36,599 Da
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 NCBI Official Full Name    tartrate-resistant acid phosphatase type 5
 NCBI Official Synonym Full Names    acid phosphatase 5, tartrate resistant
 NCBI Official Symbol    ACP5 [Similar Products]
 NCBI Official Synonym Symbols   
TRAP; SPENCDI
[Similar Products]
 NCBI Protein Information    tartrate-resistant acid phosphatase type 5; TrATPase; tartrate-resistant acid ATPase
 UniProt Protein Name    Tartrate-resistant acid phosphatase type 5
 UniProt Synonym Protein Names   
Tartrate-resistant acid ATPase; TrATPase; Type 5 acid phosphatase
 UniProt Gene Name    ACP5 [Similar Products]
 UniProt Synonym Gene Names    TR-AP; TrATPase [Similar Products]
 UniProt Entry Name    PPA5_HUMAN
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 NCBI Summary for TRACP-5b    This gene encodes an iron containing glycoprotein which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. [provided by RefSeq, Aug 2008]
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 UniProt Comments for TRACP-5b    ACP5: Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias. Defects in ACP5 are the cause of spondyloenchondrodysplasia with immune dysregulation (SPENCDI). A disease characterized by vertebral and metaphyseal dysplasia, spasticity with cerebral calcifications, and strong predisposition to autoimmune diseases. The skeletal dysplasia is characterized by radiolucent and irregular spondylar and metaphyseal lesions that represent islands of chondroid tissue within bone. ACP5 inactivating mutations result in a functional excess of phosphorylated osteopontin causing deregulation of osteopontin signaling and consequential autoimmune disease. Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.

Protein type: Phosphatase; EC 3.1.3.2; Cofactor and Vitamin Metabolism - riboflavin; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: lysosome; integral to membrane; cytosol

Molecular Function: acid phosphatase activity; ferric iron binding; ferrous iron binding

Biological Process: negative regulation of interleukin-12 production; vitamin metabolic process; negative regulation of nitric oxide biosynthetic process; response to lipopolysaccharide; negative regulation of superoxide release; negative regulation of interleukin-1 beta production; negative regulation of tumor necrosis factor production; riboflavin metabolic process; defense response to Gram-positive bacterium; dephosphorylation; response to cytokine stimulus; negative regulation of inflammatory response; water-soluble vitamin metabolic process; bone resorption

Disease: Spondyloenchondrodysplasia With Immune Dysregulation
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 Research Articles on TRACP-5b    1. TRACP5b has limited utility as a single marker of metabolic bone disease treatment.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TRACP-5b elisa kitPathways associated with TRACP-5b elisa kit
 Reference Product  PubMed Publications
 CSF1R elisa kit  >7 publications with TRACP-5b and CSF1R
 SPP1 elisa kit  >6 publications with TRACP-5b and SPP1
 Products by Pathway  Pathway Diagram
 Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway antibodies  Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway Diagram
 Defective BTD Causes Biotidinase Deficiency Pathway antibodies  Defective BTD Causes Biotidinase Deficiency Pathway Diagram
 Defective CD320 Causes Methylmalonic Aciduria Pathway antibodies  Defective CD320 Causes Methylmalonic Aciduria Pathway Diagram
 Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway antibodies  Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway Diagram
 Defective GIF Causes Intrinsic Factor Deficiency Pathway antibodies  Defective GIF Causes Intrinsic Factor Deficiency Pathway Diagram
 Defective HLCS Causes Multiple Carboxylase Deficiency Pathway antibodies  Defective HLCS Causes Multiple Carboxylase Deficiency Pathway Diagram
 Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway antibodies  Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway Diagram
 Defective MMAA Causes Methylmalonic Aciduria Type CblA Pathway antibodies  Defective MMAA Causes Methylmalonic Aciduria Type CblA Pathway Diagram
 Defective MMAB Causes Methylmalonic Aciduria Type CblB Pathway antibodies  Defective MMAB Causes Methylmalonic Aciduria Type CblB Pathway Diagram
 Defective MMACHC Causes Methylmalonic Aciduria And Homocystinuria Type CblC Pathway antibodies  Defective MMACHC Causes Methylmalonic Aciduria And Homocystinuria Type CblC Pathway Diagram
Diseases associated with TRACP-5b elisa kitOrgans/Tissues associated with TRACP-5b elisa kit
 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >4 publications with TRACP-5b and Liver Diseases
 Inflammation Antibodies  >4 publications with TRACP-5b and Inflammation
 Necrosis Antibodies  >3 publications with TRACP-5b and Necrosis
 Liver Neoplasms Antibodies  >2 publications with TRACP-5b and Liver Neoplasms
 Carcinoma, Hepatocellular Antibodies  >2 publications with TRACP-5b and Carcinoma, Hepatocellular
 Lung Diseases Antibodies  >2 publications with TRACP-5b and Lung Diseases
 Atherosclerosis Antibodies  >2 publications with TRACP-5b and Atherosclerosis
 Spondyloenchondrodysplasia Antibodies  >2 publications with TRACP-5b and Spondyloenchondrodysplasia
 Obesity Antibodies  >2 publications with TRACP-5b and Obesity
 Cardiovascular Diseases Antibodies  >1 publications with TRACP-5b and Cardiovascular Diseases
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >42 publications with TRACP-5b and Bone
 Blood Antibodies  >9 publications with TRACP-5b and Blood
 Liver Antibodies  >8 publications with TRACP-5b and Liver
 Connective Tissue Antibodies  >7 publications with TRACP-5b and Connective Tissue
 Lung Antibodies  >3 publications with TRACP-5b and Lung
 Uterus Antibodies  >2 publications with TRACP-5b and Uterus
 Kidney Antibodies  >2 publications with TRACP-5b and Kidney
 Brain Antibodies  >2 publications with TRACP-5b and Brain
 Eye Antibodies  >1 publications with TRACP-5b and Eye
 Parathyroid Antibodies  >1 publications with TRACP-5b and Parathyroid
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