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HSD17B3 elisa kit :: Mouse Testosterone ELISA Kit

Scan QR to view Datasheet Catalog #    MBS267437
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $275 +1 FREE 8GB USB
96-Strip-Wells  /  $435 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,755 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,150 +3 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Testosterone (HSD17B3), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Testosterone ELISA Kit

 Product Gene Name   

HSD17B3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    605573
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 Species Reactivity    Mouse
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 Specificity    No cross-reaction with other factors.
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 Samples    Serum, plasma or cell culture supernatant
 Assay Type    Quantitative Sandwich
 Detection Range    10 ng/ml-0.156 ng/ml
 Sensitivity    Up to 0.05 ng/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of HSD17B3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HSD17B3 purchase    MBS267437 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Testosterone (HSD17B3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 10 ng/ml-0.156 ng/ml in biological research samples containing HSD17B3, with an estimated sensitivity of Up to 0.05 ng/ml. The ELISA analytical biochemical technique of the MBS267437 kit is based on HSD17B3 antibody-HSD17B3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HSD17B3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HSD17B3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma or cell culture supernatant. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for HSD17B3 elisa kit

   Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse Testosterone monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Standard Curve/Testing Data of HSD17B3 elisa kit    HSD17B3 elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS267437. Click to request current manual
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NCBI/Uniprot data below describe general gene information for HSD17B3. It may not necessarily be applicable to this product.
 NCBI GI #    4557649
 NCBI GeneID    3293
 NCBI Accession #    NP_000188.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000197.1 [Other Products]
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 UniProt Related Accession #    P37058 [Other Products]
 Molecular Weight    34,516 Da [Similar Products]
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 NCBI Official Full Name    testosterone 17-beta-dehydrogenase 3
 NCBI Official Synonym Full Names    hydroxysteroid (17-beta) dehydrogenase 3
 NCBI Official Symbol    HSD17B3 [Similar Products]
 NCBI Official Synonym Symbols   
EDH17B3; SDR12C2
[Similar Products]
 NCBI Protein Information    testosterone 17-beta-dehydrogenase 3; 17-beta-HSD3; 17-beta-HSD 3; 17-beta-hydroxysteroid dehydrogenase type 3; testicular 17-beta-hydroxysteroid dehydrogenase; short chain dehydrogenase/reductase family 12C, member 2
 UniProt Protein Name    Testosterone 17-beta-dehydrogenase 3
 UniProt Synonym Protein Names   
17-beta-hydroxysteroid dehydrogenase type 3; 17-beta-HSD 3; Testicular 17-beta-hydroxysteroid dehydrogenase
 Protein Family    Testosterone 17-beta-dehydrogenase
 UniProt Gene Name    HSD17B3 [Similar Products]
 UniProt Synonym Gene Names    EDH17B3; 17-beta-HSD 3 [Similar Products]
 UniProt Entry Name    DHB3_HUMAN
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 NCBI Summary for HSD17B3    This isoform of 17 beta-hydroxysteroid dehydrogenase is expressed predominantly in the testis and catalyzes the conversion of androstenedione to testosterone. It preferentially uses NADP as cofactor. Deficiency can result in male pseudohermaphroditism with gynecomastia. [provided by RefSeq, Jul 2008]
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 UniProt Comments for HSD17B3    HSD17B3: Favors the reduction of androstenedione to testosterone. Uses NADPH while the two other EDH17B enzymes use NADH. Defects in HSD17B3 are the cause of male pseudohermaphrodism with gynecomastia (MPH). These individuals have unambiguous female external genitalia at birth, but fail to menstruate at the time of expected puberty and instead virilize as evidenced by growth of the phallus. Breast development may or may not take place. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.

Protein type: Lipid Metabolism - androgen and estrogen; EC; Oxidoreductase

Chromosomal Location of Human Ortholog: 9q22

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle

Molecular Function: testosterone 17-beta-dehydrogenase (NADP+) activity

Biological Process: steroid metabolic process; male genitalia development; androgen biosynthetic process

Disease: 17-beta Hydroxysteroid Dehydrogenase Iii Deficiency
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 Research Articles on HSD17B3    1. These results suggest that the HSD17B3 G289S polymorphism may be a potential risk modifier for hypospadias.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with HSD17B3 elisa kitPathways associated with HSD17B3 elisa kit
 Reference Product  PubMed Publications
 CYP17A1 elisa kit  >24 publications with HSD17B3 and CYP17A1
 SRD5A2 elisa kit  >15 publications with HSD17B3 and SRD5A2
 CYP19A1 elisa kit  >13 publications with HSD17B3 and CYP19A1
 HSD3B1 elisa kit  >12 publications with HSD17B3 and HSD3B1
 SRD5A1 elisa kit  >8 publications with HSD17B3 and SRD5A1
 HSD3B2 elisa kit  >5 publications with HSD17B3 and HSD3B2
 UGT2B17 elisa kit  >3 publications with HSD17B3 and UGT2B17
 UGT2B15 elisa kit  >3 publications with HSD17B3 and UGT2B15
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 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Metabolism Of Steroid Hormones And Vitamin D Pathway antibodies  Metabolism Of Steroid Hormones And Vitamin D Pathway Diagram
 Prostate Cancer Pathway antibodies  Prostate Cancer Pathway Diagram
 Steroid Biosynthesis Pathway antibodies  Steroid Biosynthesis Pathway Diagram
 Steroid Hormone Biosynthesis Pathway antibodies  Steroid Hormone Biosynthesis Pathway Diagram
Diseases associated with HSD17B3 elisa kitOrgans/Tissues associated with HSD17B3 elisa kit
 Disease Name  Pubmed Publications
 Urogenital Abnormalities Antibodies  >21 publications with HSD17B3 and Urogenital Abnormalities
 Disorders of Sex Development Antibodies  >19 publications with HSD17B3 and Disorders of Sex Development
 Genital Diseases, Male Antibodies  >15 publications with HSD17B3 and Genital Diseases, Male
 Prostatic Diseases Antibodies  >10 publications with HSD17B3 and Prostatic Diseases
 Prostatic Neoplasms Antibodies  >9 publications with HSD17B3 and Prostatic Neoplasms
 Hyperplasia Antibodies  >4 publications with HSD17B3 and Hyperplasia
 Ovarian Diseases Antibodies  >3 publications with HSD17B3 and Ovarian Diseases
 Prenatal Exposure Delayed Effects Antibodies  >3 publications with HSD17B3 and Prenatal Exposure Delayed Effects
 Infertility, Male Antibodies  >3 publications with HSD17B3 and Infertility, Male
 17-Hydroxysteroid Dehydrogenase Deficiency Antibodies  >2 publications with HSD17B3 and 17-Hydroxysteroid Dehydrogenase Deficiency
 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >28 publications with HSD17B3 and Testis
 Brain Antibodies  >6 publications with HSD17B3 and Brain
 Placenta Antibodies  >2 publications with HSD17B3 and Placenta
 Uncharacterized Tissue Antibodies  >1 publications with HSD17B3 and Uncharacterized Tissue
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