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TSLP elisa kit :: Mouse Thymic Stromal Lymphopoietin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS034398 TSLP elisa kit
Unit / Price
48-Strip-Wells  /  $435 +1 FREE 8GB USB
96-Strip-Wells  /  $600 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,650 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,205 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Thymic Stromal Lymphopoietin (TSLP), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Thymic Stromal Lymphopoietin ELISA Kit

 Product Gene Name   

TSLP elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: 5; NC_000005.9 (110405778..110413722). Location: 5q22.1
 OMIM    607003
 3D Structure    ModBase 3D Structure for Q969D9
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 Species Reactivity    Mouse
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 Specificity    No significant cross-reactivity or interference between Mouse TSLP and analogues was observed.
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 Samples    Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
 Assay Type    Sandwich
 Detection Range    6.25 pg/ml - 200 pg/ml.
 Sensitivity    1.0 pg/ml.
 Intended Uses    This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated TSLP concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse TSLP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TSLP and TSLP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TSLP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Intra-assay Precision    Intra-assay CV (%) is less than 15%.
 Inter-assay Precision    Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TSLP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TSLP purchase    MBS034398 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Thymic Stromal Lymphopoietin (TSLP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 6.25 pg/ml - 200 pg/ml in biological research samples containing TSLP, with an estimated sensitivity of 1.0 pg/ml. The ELISA analytical biochemical technique of the MBS034398 kit is based on TSLP antibody-TSLP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TSLP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TSLP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for TSLP elisa kit

   Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated TSLP concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse TSLP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TSLP and TSLP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TSLP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS034398. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TSLP. It may not necessarily be applicable to this product.
 NCBI GI #    190886449
 NCBI GeneID    85480
 NCBI Accession #    NP_612561.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_138551.4 [Other Products]
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 UniProt Primary Accession #    Q969D9 [Other Products]
 UniProt Secondary Accession #    Q8IW99 [Other Products]
 UniProt Related Accession #    Q969D9 [Other Products]
 Molecular Weight    18,141 Da
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 NCBI Official Full Name    thymic stromal lymphopoietin isoform 2
 NCBI Official Synonym Full Names    thymic stromal lymphopoietin
 NCBI Official Symbol    TSLP [Similar Products]
 NCBI Protein Information    thymic stromal lymphopoietin
 UniProt Protein Name    Thymic stromal lymphopoietin
 Protein Family    Thymic stromal lymphopoietin
 UniProt Gene Name    TSLP [Similar Products]
 UniProt Entry Name    TSLP_HUMAN
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 NCBI Summary for TSLP    This gene encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c(+) dendritic cells. The protein promotes T helper type 2 (TH2) cell responses that are associated with immunity in various inflammatory diseases, including asthma, allergic inflammation and chronic obstructive pulmonary disease. The protein is therefore considered a potential therapeutic target for the treatment of such diseases. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2012]
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 UniProt Comments for TSLP    TSLP: Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 5q22.1

Cellular Component: extracellular space

Molecular Function: cytokine activity
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 Research Articles on TSLP    1. This study suggests that SNPs in TSLP gene may exert a gender and/or nasal polyposis-dependent risk for development of CRS in Chinese subjects.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TSLP elisa kitPathways associated with TSLP elisa kit
 Reference Product  PubMed Publications
 CRLF2 elisa kit  >49 publications with TSLP and CRLF2
 TNFSF4 elisa kit  >29 publications with TSLP and TNFSF4
 IL13 elisa kit  >26 publications with TSLP and IL13
 FOXP3 elisa kit  >22 publications with TSLP and FOXP3
 CCL17 elisa kit  >14 publications with TSLP and CCL17
 IL7R elisa kit  >5 publications with TSLP and IL7R
 IL4 elisa kit  >5 publications with TSLP and IL4
 IL9 elisa kit  >1 publications with TSLP and IL9
 Products by Pathway  Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 TSLP Signaling Pathway antibodies  TSLP Signaling Pathway Diagram
Diseases associated with TSLP elisa kitOrgans/Tissues associated with TSLP elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >220 publications with TSLP and Inflammation
 Asthma Antibodies  >154 publications with TSLP and Asthma
 Dermatitis, Atopic Antibodies  >119 publications with TSLP and Dermatitis, Atopic
 Lung Diseases Antibodies  >99 publications with TSLP and Lung Diseases
 Necrosis Antibodies  >50 publications with TSLP and Necrosis
 Disease Models, Animal Antibodies  >44 publications with TSLP and Disease Models, Animal
 Drug Hypersensitivity Antibodies  >13 publications with TSLP and Drug Hypersensitivity
 Hyperplasia Antibodies  >12 publications with TSLP and Hyperplasia
 Glomerulonephritis, Membranoproliferative Antibodies  >8 publications with TSLP and Glomerulonephritis, Membranoproliferative
 Cardiovascular Diseases Antibodies  >7 publications with TSLP and Cardiovascular Diseases
 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >115 publications with TSLP and Skin
 Lung Antibodies  >104 publications with TSLP and Lung
 Bone Marrow Antibodies  >22 publications with TSLP and Bone Marrow
 Eye Antibodies  >7 publications with TSLP and Eye
 Connective Tissue Antibodies  >1 publications with TSLP and Connective Tissue
 Thyroid Antibodies  >1 publications with TSLP and Thyroid
 Brain Antibodies  >1 publications with TSLP and Brain
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