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Tbeta4 elisa kit :: Mouse Thymosin Beta 4 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS721916
 Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Thymosin Beta 4 (Tbeta4), ELISA Kit

 Also Known As   

Mouse Thymosin Beta 4 ELISA Kit

 Product Gene Name   

Tbeta4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    300159
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Tbeta4. No significant cross-reactivity or interference between Tbeta4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Tbeta4 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Quantitative Competitive
 Sensitivity    1.0 ng/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Tbeta4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Tbeta4 purchase    MBS721916 is a ready-to-use microwell, strip plate Quantitative Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Thymosin Beta 4 (Tbeta4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Tbeta4, with an estimated sensitivity of 1.0 ng/mL. The ELISA analytical biochemical technique of the MBS721916 kit is based on Tbeta4 antibody-Tbeta4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Tbeta4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Tbeta4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for Tbeta4 elisa kit

   Intended Uses: This Tbeta4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse Tbeta4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: Tbeta4 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-Tbeta4 antibody and an Tbeta4-HRP conjugate. The assay sample and buffer are incubated together with Tbeta4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Tbeta4 concentration since Tbeta4 from samples and Tbeta4-HRP conjugate compete for the anti-Tbeta4 antibody binding site. Since the number of sites is limited, as more sites are occupied by Tbeta4 from the sample, fewer sites are left to bind Tbeta4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Tbeta4 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for Tbeta4 elisa kit    Signal Transduction
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  Typical Testing Data/Standard Curve (for reference only) of Tbeta4 elisa kit    Tbeta4 elisa kit  Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS721916. Click to request current manual
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NCBI/Uniprot data below describe general gene information for Tbeta4. It may not necessarily be applicable to this product.
 NCBI GI #    11056061
 NCBI GeneID    7114
 NCBI Accession #    NP_066932.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_021109.3 [Other Products]
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 UniProt Secondary Accession #    P01253; P01254; Q546P5; Q63576; Q9UE55 [Other Products]
 UniProt Related Accession #    P62328 [Other Products]
 Molecular Weight    5,053 Da
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 NCBI Official Full Name    thymosin beta-4
 NCBI Official Synonym Full Names    thymosin beta 4, X-linked
 NCBI Official Symbol    TMSB4X [Similar Products]
 NCBI Official Synonym Symbols   
FX; TB4X; PTMB4; TMSB4
[Similar Products]
 NCBI Protein Information    thymosin beta-4; t beta-4; prothymosin beta-4; thymosin, beta 4, X chromosome
 UniProt Protein Name    Thymosin beta-4
 UniProt Synonym Protein Names   
FxCleaved into the following chain:Hematopoietic system regulatory peptideAlternative name(s):Seraspenide
 UniProt Gene Name    TMSB4X [Similar Products]
 UniProt Synonym Gene Names    TB4X; THYB4; TMSB4; T beta-4 [Similar Products]
 UniProt Entry Name    TYB4_HUMAN
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 NCBI Summary for Tbeta4    This gene encodes an actin sequestering protein which plays a role in regulation of actin polymerization. The protein is also involved in cell proliferation, migration, and differentiation. This gene escapes X inactivation and has a homolog on chromosome Y. [provided by RefSeq, Jul 2008]
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 UniProt Comments for Tbeta4    TMSB4X: Plays an important role in the organization of the cytoskeleton. Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization. Belongs to the thymosin beta family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: Xq21.3-q22

Cellular Component: cytoskeleton; extracellular region

Molecular Function: actin monomer binding; protein binding

Biological Process: platelet activation; platelet degranulation; sequestering of actin monomers; actin filament organization; blood coagulation
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 Research Articles on Tbeta4    1. Thymosin beta-4 levels in the serum and synovial fluid of knee osteoarthritis patients are significantly correlated with disease severity.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with Tbeta4 elisa kitDiseases associated with Tbeta4 elisa kit
 Products by Pathway  Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Platelet Activation, Signaling And Aggregation Pathway antibodies  Platelet Activation, Signaling And Aggregation Pathway Diagram
 Platelet Degranulation Pathway antibodies  Platelet Degranulation Pathway Diagram
 Regulation Of Actin Cytoskeleton Pathway antibodies  Regulation Of Actin Cytoskeleton Pathway Diagram
 Regulation Of Actin Cytoskeleton Pathway antibodies  Regulation Of Actin Cytoskeleton Pathway Diagram
 Regulation Of Actin Cytoskeleton Pathway antibodies  Regulation Of Actin Cytoskeleton Pathway Diagram
 Response To Elevated Platelet Cytosolic Ca2+ Pathway antibodies  Response To Elevated Platelet Cytosolic Ca2+ Pathway Diagram
 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >3 publications with Tbeta4 and Disease Models, Animal
 Carcinoma Antibodies  >2 publications with Tbeta4 and Carcinoma
 Neoplasms, Experimental Antibodies  >2 publications with Tbeta4 and Neoplasms, Experimental
 Adenocarcinoma Antibodies  >1 publications with Tbeta4 and Adenocarcinoma
 Liver Diseases Antibodies  >1 publications with Tbeta4 and Liver Diseases
 Liver Neoplasms, Experimental Antibodies  >1 publications with Tbeta4 and Liver Neoplasms, Experimental
 Liver Neoplasms Antibodies  >1 publications with Tbeta4 and Liver Neoplasms
 Carcinoma, Hepatocellular Antibodies  >1 publications with Tbeta4 and Carcinoma, Hepatocellular
 Inflammation Antibodies  >1 publications with Tbeta4 and Inflammation
Organs/Tissues associated with Tbeta4 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Mouth Antibodies  >1 publications with Tbeta4 and Mouth
 Bone Antibodies  >1 publications with Tbeta4 and Bone
 Testis Antibodies  >1 publications with Tbeta4 and Testis
 Embryonic Tissue Antibodies  >1 publications with Tbeta4 and Embryonic Tissue
 Heart Antibodies  >1 publications with Tbeta4 and Heart
 Blood Antibodies  >1 publications with Tbeta4 and Blood
 Liver Antibodies  >1 publications with Tbeta4 and Liver
 Vascular Antibodies  >1 publications with Tbeta4 and Vascular
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