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TMEM27 elisa kit :: Mouse Transmembrane Protein 27 (TMEM27) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2021452 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $270 +1 FREE 8GB USB
48-Strip-Wells  /  $505 +1 FREE 8GB USB
96-Strip-Wells  /  $685 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,770 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,025 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Transmembrane Protein 27 (TMEM27), ELISA Kit

 Also Known As   

Transmembrane Protein 27 (TMEM27) ELISA Kit

 Product Synonym Names    NX17; Collectrin
 Product Gene Name   

TMEM27 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    300631
 3D Structure    ModBase 3D Structure for Q9HBJ8
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Transmembrane Protein 27 (TMEM27).
No significant cross-reactivity or interference between Transmembrane Protein 27 (TMEM27) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
 Detection Range    0.156-10ng/mL
 Sensitivity    < 0.058ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Transmembrane Protein 27 (TMEM27) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Transmembrane Protein 27 (TMEM27) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TMEM27 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TMEM27 purchase    MBS2021452 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Transmembrane Protein 27 (TMEM27) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing TMEM27, with an estimated sensitivity of < 0.058ng/mL. The ELISA analytical biochemical technique of the MBS2021452 kit is based on TMEM27 antibody-TMEM27 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TMEM27 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TMEM27. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for TMEM27 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transmembrane Protein 27 (TMEM27). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transmembrane Protein 27 (TMEM27). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transmembrane Protein 27 (TMEM27), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transmembrane Protein 27 (TMEM27) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for TMEM27 elisa kit    Signal transduction
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 Typical Testing Data/Standard Curve (for reference only) of TMEM27 elisa kit    TMEM27 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2021452. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TMEM27. It may not necessarily be applicable to this product.
 NCBI GI #    119619300
 NCBI GeneID    57393
 NCBI Accession #    EAW98894.1 [Other Products]
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 UniProt Primary Accession #    Q9HBJ8 [Other Products]
 UniProt Secondary Accession #    Q6UW07; B2R9M1 [Other Products]
 UniProt Related Accession #    Q9HBJ8 [Other Products]
 Molecular Weight    25,235 Da
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 NCBI Official Full Name    transmembrane protein 27
 NCBI Official Synonym Full Names    transmembrane protein 27
 NCBI Official Symbol    TMEM27 [Similar Products]
 NCBI Official Synonym Symbols   
NX17; NX-17
[Similar Products]
 NCBI Protein Information    collectrin
 UniProt Protein Name    Collectrin
 UniProt Synonym Protein Names   
Transmembrane protein 27
 Protein Family    Collectrin
 UniProt Gene Name    TMEM27 [Similar Products]
 UniProt Entry Name    TMM27_HUMAN
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 NCBI Summary for TMEM27    This gene encodes a type 1 transmembrane protein that is important for trafficking amino acid transporters to the apical brush border of proximal tubules. The encoded protein binds to amino acid transporters and regulates their expression on the plasma membrane. It also plays a role in controlling insulin exocytosis by regulating formation of the SNARE (soluble N-ethylmaleimide-sensitive-factor attachment protein receptor) complex in pancreatic beta cells. The extracellular domain of the encoded protein may be cleaved and shed from the plasma membrane specifically in pancreatic beta cells. [provided by RefSeq, Jun 2013]
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 UniProt Comments for TMEM27    TMEM27: Regulator of SNARE complex function. Stimulator of beta cell replication. Belongs to the TMEM27 family.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: Xp22

Cellular Component: brush border membrane; cytoplasm; integral to membrane

Molecular Function: peptidyl-dipeptidase activity; protein binding; metallopeptidase activity

Biological Process: positive regulation of calcium ion-dependent exocytosis; proteolysis; positive regulation of amino acid transport
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 Research Articles on TMEM27    1. Tmem27 is present in human serum and its levels are significantly lower in subjects with autoimmune diabetes as compared to healthy individuals.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TMEM27 elisa kitDiseases associated with TMEM27 elisa kit
 Reference Product  PubMed Publications
 SLC6A19 elisa kit  >8 publications with TMEM27 and SLC6A19
 SNAPIN elisa kit  >3 publications with TMEM27 and SNAPIN
 SLC6A20 elisa kit  >1 publications with TMEM27 and SLC6A20
 SLC3A1 elisa kit  >1 publications with TMEM27 and SLC3A1
 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >11 publications with TMEM27 and Kidney Diseases
 Brain Diseases Antibodies  >6 publications with TMEM27 and Brain Diseases
 Hypertension Antibodies  >5 publications with TMEM27 and Hypertension
 Heart Diseases Antibodies  >1 publications with TMEM27 and Heart Diseases
 Liver Diseases Antibodies  >1 publications with TMEM27 and Liver Diseases
 Fibrosis Antibodies  >1 publications with TMEM27 and Fibrosis
 Pancreatic Neoplasms Antibodies  >1 publications with TMEM27 and Pancreatic Neoplasms
 Inflammation Antibodies  >1 publications with TMEM27 and Inflammation
Organs/Tissues associated with TMEM27 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >26 publications with TMEM27 and Kidney
 Blood Antibodies  >8 publications with TMEM27 and Blood
 Lung Antibodies  >3 publications with TMEM27 and Lung
 Liver Antibodies  >2 publications with TMEM27 and Liver
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