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| Product Name
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Tyrosine Hydroxylase (TH), ELISA Kit ★Popular Item★
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| Also Known As
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Mouse Tyrosine Hydroxylase ELISA Kit
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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 TOP
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| Sample Manual Insert
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| Request for Current Manual Insert
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| OMIM
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gene 605407
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| Species Reactivity
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Mouse
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| Specificity
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This assay has high sensitivity and excellent specificity for detection of TH. No significant cross-reactivity or interference between TH and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TH and all the analogues, therefore, cross reaction may still exist in some cases.
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| Samples
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Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
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| Assay Type
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Quantitative Competitive
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| Sensitivity
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0.1 ng/mL.
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| Preparation and Storage
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Store all reagents at 2-8 degree C.
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| Sample Preparation
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We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
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| Product Note
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Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
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| Other Notes
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Small volumes of TH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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| Searchable Terms for TH purchase
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MBS738113 is a ready-to-use microwell, strip plate Quantitative Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Tyrosine Hydroxylase (TH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TH, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS738113 kit is based on TH antibody-TH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for TH elisa kit
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Intended Uses: This TH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse TH. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay: TH ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-TH antibody and an TH-HRP conjugate. The assay sample and buffer are incubated together with TH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TH concentration since TH from samples and TH-HRP conjugate compete for the anti-TH antibody binding site. Since the number of sites is limited, as more sites are occupied by TH from the sample, fewer sites are left to bind TH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TH concentration in each sample is interpolated from this standard curve.
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| Product Categories/Family for TH elisa kit
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Neurobiology
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| Typical Testing Data/Standard Curve (for reference only) of TH elisa kit
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| Sample Manual Insert of MBS738113. Click to request current manual
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| NCBI/Uniprot data below describe general gene information for TH. It may not necessarily be applicable to this product. |
| NCBI GI #
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986909
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| NCBI GeneID
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7054
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| NCBI Accession #
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AAA77650.1
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[Other Products]
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| UniProt Secondary Accession #
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Q0PWM2; Q0PWM3; Q15585; Q15588; Q15589; Q2M3B4; B7ZL70; B7ZL73
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[Other Products]
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| UniProt Related Accession #
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P07101
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[Other Products]
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| Molecular Weight
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44,898 Da
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TOP
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| NCBI Official Full Name
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tyrosine hydroxylase, partial
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| NCBI Official Synonym Full Names
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tyrosine hydroxylase
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| NCBI Official Symbol
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TH
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[Similar Products]
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| NCBI Official Synonym Symbols
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TYH; DYT14; DYT5b
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[Similar Products]
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| NCBI Protein Information
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tyrosine 3-monooxygenase; dystonia 14; tyrosine 3-hydroxylase
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| UniProt Protein Name
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Tyrosine 3-monooxygenase
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| UniProt Synonym Protein Names
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Tyrosine 3-hydroxylase; TH
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| UniProt Gene Name
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TH
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[Similar Products]
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| UniProt Synonym Gene Names
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TYH; TH
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[Similar Products]
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| UniProt Entry Name
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TY3H_HUMAN
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| NCBI Summary for TH
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The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jul 2008]
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| UniProt Comments for TH
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TH: an enzyme involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Four splice variant isoforms have been described.
Protein type: Amino Acid Metabolism - tyrosine; Oxidoreductase; Vesicle; EC 1.14.16.2; Mitochondrial; Endoplasmic reticulum
Chromosomal Location of Human Ortholog: 11p15.5
Cellular Component: synaptic vesicle; internal side of plasma membrane; neuron projection; smooth endoplasmic reticulum; mitochondrion; dendrite; melanosome membrane; cytoplasm; terminal button; perikaryon; cytoplasmic vesicle; cytosol; nucleus
Molecular Function: amino acid binding; protein domain specific binding; protein binding; enzyme binding; ferric iron binding; ferrous iron binding; dopamine binding; oxygen binding; tyrosine 3-monooxygenase activity
Biological Process: heart morphogenesis; response to peptide hormone stimulus; synaptic transmission, dopaminergic; heart development; response to lipopolysaccharide; locomotory behavior; dopamine biosynthetic process; fatty acid metabolic process; response to salt stress; phytoalexin metabolic process; response to estradiol stimulus; regulation of heart contraction; norepinephrine biosynthetic process; dopamine biosynthetic process from tyrosine; sensory perception of sound; catecholamine biosynthetic process; visual perception; response to electrical stimulus; epinephrine biosynthetic process; glycoside metabolic process; response to corticosterone stimulus; neurotransmitter biosynthetic process; response to pyrethroid; response to nutrient levels; response to light stimulus; anatomical structure morphogenesis; terpene metabolic process; sphingolipid metabolic process; eating behavior; phthalate metabolic process; response to amphetamine; mating behavior; multicellular organismal aging; isoquinoline alkaloid metabolic process; learning; social behavior; response to herbicide; response to ether; synaptic vesicle amine transport; memory; organ morphogenesis; eye photoreceptor cell development; response to ethanol; circadian sleep/wake cycle; pigmentation; response to zinc ion; response to hypoxia; embryonic camera-type eye morphogenesis; cerebral cortex development; response to activity; response to water deprivation
Disease: Segawa Syndrome, Autosomal Recessive
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| Research Articles on TH
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1. Achilles tendon tenocytes produce tyrosine hydroxylase.
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Products associated with TH elisa kit | Pathways associated with TH elisa kit |
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| Diseases associated with TH elisa kit | Organs/Tissues associated with TH elisa kit |
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ELISA Kit 
