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vWF elisa kit :: Mouse Von Willebrand Factor ELISA Kit

Scan QR to view Datasheet Catalog #    MBS282127 vWF elisa kit
Unit / Price
48-Strip-Wells  /  $425 +1 FREE 8GB USB
96-Strip-Wells  /  $720 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,160 +3 FREE 8GB USB
10x96-Strip-Wells  /  $6,055 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Von Willebrand Factor (vWF), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Von Willebrand Factor (vWF) ELISA Kit

 Product Gene Name   

vWF elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for Q8CIZ8
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Mouse VWF. No significant cross-reactivity or interference between Mouse VWF and analogues was observed.
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 Samples    Serum, Plasma, Other biological fluids
 Assay Type    Sandwich
 Sample Volume    1-200 uL
 Precision    Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
 Detection Wavelength    450 nm
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 Preparation and Storage    Store at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of vWF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for vWF purchase    MBS282127 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Von Willebrand Factor (vWF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing vWF. The ELISA analytical biochemical technique of the MBS282127 kit is based on vWF antibody-vWF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect vWF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, vWF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for vWF elisa kit

   Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate VWF in samples. An antibody specific for VWF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVWF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VWF is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VWF bound in the initial step. The color development is stopped and the intensity of the color is measured.
Sample Manual Insert of MBS282127. Click to request current manual
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NCBI/Uniprot data below describe general gene information for vWF. It may not necessarily be applicable to this product.
 NCBI GI #    115511022
 NCBI GeneID    22371
 NCBI Accession #    NP_035838.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_011708.4 [Other Products]
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 UniProt Primary Accession #    Q8CIZ8 [Other Products]
 UniProt Secondary Accession #    Q60863; Q6XUV6; Q8BIU9; Q8CGN0; Q9JK16 [Other Products]
 UniProt Related Accession #    Q8CIZ8 [Other Products]
 Molecular Weight    44,467 Da
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 NCBI Official Full Name    von Willebrand factor
 NCBI Official Synonym Full Names    Von Willebrand factor
 NCBI Official Symbol    Vwf [Similar Products]
 NCBI Official Synonym Symbols   
VWD; F8VWF; AI551257; C630030D09; 6820430P06Rik; B130011O06Rik
[Similar Products]
 NCBI Protein Information    von Willebrand factor
 UniProt Protein Name    von Willebrand factor
 UniProt Synonym Protein Names   
von Willebrand antigen 2Alternative name(s):von Willebrand antigen II
 Protein Family    von Willebrand factor
 UniProt Gene Name    Vwf [Similar Products]
 UniProt Synonym Gene Names    vWF [Similar Products]
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 UniProt Comments for vWF    VWF: Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma. Defects in VWF are the cause of von Willebrand disease type 1 (VWD1). A common hemorrhagic disorder due to defects in von Willebrand factor protein and resulting in impaired platelet aggregation. Von Willebrand disease type 1 is characterized by partial quantitative deficiency of circulating von Willebrand factor, that is otherwise structurally and functionally normal. Clinical manifestations are mucocutaneous bleeding, such as epistaxis and menorrhagia, and prolonged bleeding after surgery or trauma. Defects in VWF are the cause of von Willebrand disease type 2 (VWD2). A hemorrhagic disorder due to defects in von Willebrand factor protein and resulting in impaired platelet aggregation. Von Willebrand disease type 2 is characterized by qualitative deficiency and functional anomalies of von Willebrand factor. It is divided in different subtypes including 2A, 2B, 2M and 2N (Normandy variant). The mutant VWF protein in types 2A, 2B and 2M are defective in their platelet- dependent function, whereas the mutant protein in type 2N is defective in its ability to bind factor VIII. Clinical manifestations are mucocutaneous bleeding, such as epistaxis and menorrhagia, and prolonged bleeding after surgery or trauma. Defects in VWF are the cause of von Willebrand disease type 3 (VWD3). A severe hemorrhagic disorder due to a total or near total absence of von Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII. Bleeding usually starts in infancy and can include epistaxis, recurrent mucocutaneous bleeding, excessive bleeding after minor trauma, and hemarthroses.

Protein type: Cell adhesion; Extracellular matrix; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6 F3|6 59.32 cM

Cellular Component: cytoplasm; endoplasmic reticulum; external side of plasma membrane; extracellular matrix; extracellular region; extracellular space; proteinaceous extracellular matrix

Molecular Function: chaperone binding; collagen binding; identical protein binding; immunoglobulin binding; integrin binding; protease binding; protein binding; protein homodimerization activity; protein N-terminus binding

Biological Process: blood coagulation; cell adhesion; cell-substrate adhesion; hemostasis; liver development; placenta development; platelet activation; protein homooligomerization
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 Research Articles on vWF    1. Refrigeration-induced binding of VWF to platelets facilitates their rapid clearance by inducing GPIbalpha-mediated signaling.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with vWF elisa kitPathways associated with vWF elisa kit
 Reference Product  PubMed Publications
 Vegfa elisa kit  >61 publications with vWF and Vegfa
 Serpine1 elisa kit  >33 publications with vWF and Serpine1
 Gp1ba elisa kit  >20 publications with vWF and Gp1ba
 Itgb3 elisa kit  >10 publications with vWF and Itgb3
 Itga2b elisa kit  >7 publications with vWF and Itga2b
 Gp9 elisa kit  >2 publications with vWF and Gp9
 Products by Pathway  Pathway Diagram
 Blood Clotting Cascade Pathway antibodies  Blood Clotting Cascade Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies  Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram
Diseases associated with vWF elisa kitOrgans/Tissues associated with vWF elisa kit
 Disease Name  Pubmed Publications
 Vascular Diseases Antibodies  >2148 publications with vWF and Vascular Diseases
 Cardiovascular Diseases Antibodies  >2137 publications with vWF and Cardiovascular Diseases
 Thrombosis Antibodies  >1517 publications with vWF and Thrombosis
 Inflammation Antibodies  >668 publications with vWF and Inflammation
 Heart Diseases Antibodies  >662 publications with vWF and Heart Diseases
 Atherosclerosis Antibodies  >386 publications with vWF and Atherosclerosis
 Kidney Diseases Antibodies  >339 publications with vWF and Kidney Diseases
 Hypertension Antibodies  >323 publications with vWF and Hypertension
 Disease Models, Animal Antibodies  >320 publications with vWF and Disease Models, Animal
 Necrosis Antibodies  >273 publications with vWF and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >301 publications with vWF and Brain
 Embryonic Tissue Antibodies  >281 publications with vWF and Embryonic Tissue
 Bone Marrow Antibodies  >277 publications with vWF and Bone Marrow
 Lung Antibodies  >266 publications with vWF and Lung
 Skin Antibodies  >253 publications with vWF and Skin
 Connective Tissue Antibodies  >146 publications with vWF and Connective Tissue
 Joint Antibodies  >81 publications with vWF and Joint
 Eye Antibodies  >61 publications with vWF and Eye
 Spleen Antibodies  >47 publications with vWF and Spleen
 Inner Ear Antibodies  >10 publications with vWF and Inner Ear
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