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WIF1 elisa kit :: Mouse Wnt inhibitory factor 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS282004 WIF1 elisa kit
Unit / Price
48-Strip-Wells  /  $425 +1 FREE 8GB USB
96-Strip-Wells  /  $720 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,160 +3 FREE 8GB USB
10x96-Strip-Wells  /  $6,055 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Wnt inhibitory factor 1 (WIF1), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Wnt inhibitory factor 1 (WIF1) ELISA Kit

 Product Gene Name   

WIF1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for Q9WUA1
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Mouse WIF1. No significant cross-reactivity or interference between Mouse WIF1 and analogues was observed.
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 Samples    Serum, Plasma, Other biological fluids
 Assay Type    Sandwich
 Sample Volume    1-200 uL
 Precision    Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
 Detection Wavelength    450 nm
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 Preparation and Storage    Store at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of WIF1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for WIF1 purchase    MBS282004 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Wnt inhibitory factor 1 (WIF1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing WIF1. The ELISA analytical biochemical technique of the MBS282004 kit is based on WIF1 antibody-WIF1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect WIF1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, WIF1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for WIF1 elisa kit

   Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate WIF1 in samples. An antibody specific for WIF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWIF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WIF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WIF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Sample Manual Insert of MBS282004. Click to request current manual
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NCBI/Uniprot data below describe general gene information for WIF1. It may not necessarily be applicable to this product.
 NCBI GI #    6755999
 NCBI GeneID    24117
 NCBI Accession #    NP_036045.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_011915.2 [Other Products]
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 UniProt Primary Accession #    Q9WUA1 [Other Products]
 UniProt Related Accession #    Q9WUA1 [Other Products]
 Molecular Weight    41,590 Da
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 NCBI Official Full Name    wnt inhibitory factor 1
 NCBI Official Synonym Full Names    Wnt inhibitory factor 1
 NCBI Official Symbol    Wif1 [Similar Products]
 NCBI Official Synonym Symbols   
WIF-1; AW107799
[Similar Products]
 NCBI Protein Information    wnt inhibitory factor 1
 UniProt Protein Name    Wnt inhibitory factor 1
 Protein Family    Wnt inhibitory factor
 UniProt Gene Name    Wif1 [Similar Products]
 UniProt Synonym Gene Names    WIF-1 [Similar Products]
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 UniProt Comments for WIF1    WIF1: Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation.

Protein type: Inhibitor; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10|10 D2

Cellular Component: extracellular region

Molecular Function: Wnt-protein binding

Biological Process: multicellular organism development; negative regulation of Wnt signaling pathway; positive regulation of fat cell differentiation; Wnt signaling pathway
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 Research Articles on WIF1    1. Wif1 localizes to the enamel knot in which Wif1 regulates apoptosis by mediating and regulating Wnt-beta-catenin signaling. Thus, Wif1 plays an essential role in tooth development.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with WIF1 elisa kitPathways associated with WIF1 elisa kit
 Reference Product  PubMed Publications
 Wnt5a elisa kit  >23 publications with WIF1 and Wnt5a
 Wnt1 elisa kit  >11 publications with WIF1 and Wnt1
 Wnt3a elisa kit  >8 publications with WIF1 and Wnt3a
 Wnt4 elisa kit  >6 publications with WIF1 and Wnt4
 Wnt7a elisa kit  >5 publications with WIF1 and Wnt7a
 Wnt16 elisa kit  >4 publications with WIF1 and Wnt16
 Wnt7b elisa kit  >3 publications with WIF1 and Wnt7b
 Wnt9a elisa kit  >3 publications with WIF1 and Wnt9a
 Wnt2b elisa kit  >2 publications with WIF1 and Wnt2b
 Products by Pathway  Pathway Diagram
 PodNet: Protein-protein Interactions In The Podocyte Pathway antibodies  PodNet: Protein-protein Interactions In The Podocyte Pathway Diagram
 Wnt Signaling Pathway antibodies  Wnt Signaling Pathway Diagram
 Wnt Signaling Pathway antibodies  Wnt Signaling Pathway Diagram
 XPodNet - Protein-protein Interactions In The Podocyte Expanded By STRING Pathway antibodies  XPodNet - Protein-protein Interactions In The Podocyte Expanded By STRING Pathway Diagram
Diseases associated with WIF1 elisa kitOrgans/Tissues associated with WIF1 elisa kit
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >78 publications with WIF1 and Carcinoma
 Colorectal Neoplasms Antibodies  >33 publications with WIF1 and Colorectal Neoplasms
 Lung Neoplasms Antibodies  >28 publications with WIF1 and Lung Neoplasms
 Lung Diseases Antibodies  >26 publications with WIF1 and Lung Diseases
 Adenocarcinoma Antibodies  >25 publications with WIF1 and Adenocarcinoma
 Breast Neoplasms Antibodies  >16 publications with WIF1 and Breast Neoplasms
 Neoplasms, Experimental Antibodies  >11 publications with WIF1 and Neoplasms, Experimental
 Liver Diseases Antibodies  >10 publications with WIF1 and Liver Diseases
 Colonic Neoplasms Antibodies  >10 publications with WIF1 and Colonic Neoplasms
 Carcinoma, Hepatocellular Antibodies  >9 publications with WIF1 and Carcinoma, Hepatocellular
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >44 publications with WIF1 and Bone
 Lung Antibodies  >36 publications with WIF1 and Lung
 Brain Antibodies  >20 publications with WIF1 and Brain
 Eye Antibodies  >17 publications with WIF1 and Eye
 Skin Antibodies  >16 publications with WIF1 and Skin
 Embryonic Tissue Antibodies  >14 publications with WIF1 and Embryonic Tissue
 Bladder Antibodies  >9 publications with WIF1 and Bladder
 Heart Antibodies  >6 publications with WIF1 and Heart
 Joint Antibodies  >5 publications with WIF1 and Joint
 Stomach Antibodies  >4 publications with WIF1 and Stomach
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