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ALCAM elisa kit :: Mouse activated leukocyte cell adhesion molecule ELISA Kit

Scan QR to view Datasheet Catalog #    MBS700580
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $475 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,715 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,140 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

activated leukocyte cell adhesion molecule (ALCAM), ELISA Kit

 Also Known As   

Mouse Activated Leukocyte Cell Adhesion Molecule, ALCAM ELISA Kit

 Product Synonym Names    Mouse Activated Leukocyte Cell Adhesion Molecule; ALCAM ELISA Kit; CD166; FLJ38514; MEMD; MGC71733; activated leucocyte cell adhesion molecule; activated leukocyte cell adhesion molecule
 Product Gene Name   

ALCAM elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS700580 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q61490
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse ALCAM. No significant cross-reactivity or interference between mouse ALCAM and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    1.25 ng/ml -80 ng/ml.
 Sensitivity    The minimum detectable dose of mouse ALCAM is typically less than 0.31 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ALCAM elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ALCAM purchase    MBS700580 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the activated leukocyte cell adhesion molecule (ALCAM) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.25 ng/ml -80 ng/ml in biological research samples containing ALCAM, with an estimated sensitivity of The minimum detectable dose of mouse ALCAM is typically less than 0.31 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS700580 kit is based on ALCAM antibody-ALCAM antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ALCAM antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ALCAM. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ALCAM elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ALCAM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ALCAM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ALCAM is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ALCAM bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of ALCAM elisa kit    ALCAM elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS700580. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ALCAM. It may not necessarily be applicable to this product.
 NCBI GI #    31791059
 NCBI GeneID    11658
 NCBI Accession #    NP_033785.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_009655.2 [Other Products]
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 UniProt Primary Accession #    Q61490 [Other Products]
 UniProt Secondary Accession #    O70136; Q8CDA5; Q8R2T0 [Other Products]
 UniProt Related Accession #    Q61490 [Other Products]
 Molecular Weight    65,092 Da
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 NCBI Official Full Name    CD166 antigen
 NCBI Official Synonym Full Names    activated leukocyte cell adhesion molecule
 NCBI Official Symbol    Alcam [Similar Products]
 NCBI Official Synonym Symbols   
BEN; SC1; MuSC; CD166; AI853494; DM-GRASP
[Similar Products]
 NCBI Protein Information    CD166 antigen
 UniProt Protein Name    CD166 antigen
 UniProt Synonym Protein Names   
Activated leukocyte cell adhesion molecule; Protein DM-GRASP; CD_antigen: CD166
 Protein Family    CD166 antigen
 UniProt Gene Name    Alcam [Similar Products]
 UniProt Entry Name    CD166_MOUSE
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 UniProt Comments for ALCAM    Function: Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system.
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 Research Articles on ALCAM    1. ALCAM stably interacts with actin by binding to syntenin-1 and ezrin.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ALCAM elisa kitPathways associated with ALCAM elisa kit
 Reference Product  PubMed Publications
 CD6 elisa kit  >55 publications with ALCAM and CD6
 EGFR elisa kit  >2 publications with ALCAM and EGFR
 Products by Pathway  Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 L1CAM Interactions Pathway antibodies  L1CAM Interactions Pathway Diagram
Diseases associated with ALCAM elisa kitOrgans/Tissues associated with ALCAM elisa kit
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >51 publications with ALCAM and Carcinoma
 Breast Neoplasms Antibodies  >28 publications with ALCAM and Breast Neoplasms
 Adenocarcinoma Antibodies  >23 publications with ALCAM and Adenocarcinoma
 Inflammation Antibodies  >17 publications with ALCAM and Inflammation
 Necrosis Antibodies  >15 publications with ALCAM and Necrosis
 Brain Diseases Antibodies  >14 publications with ALCAM and Brain Diseases
 Cardiovascular Diseases Antibodies  >11 publications with ALCAM and Cardiovascular Diseases
 Liver Diseases Antibodies  >9 publications with ALCAM and Liver Diseases
 Liver Neoplasms Antibodies  >9 publications with ALCAM and Liver Neoplasms
 Ovarian Neoplasms Antibodies  >8 publications with ALCAM and Ovarian Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Nerve Antibodies  >57 publications with ALCAM and Nerve
 Embryonic Tissue Antibodies  >52 publications with ALCAM and Embryonic Tissue
 Bone Antibodies  >51 publications with ALCAM and Bone
 Brain Antibodies  >48 publications with ALCAM and Brain
 Bone Marrow Antibodies  >39 publications with ALCAM and Bone Marrow
 Vascular Antibodies  >33 publications with ALCAM and Vascular
 Muscle Antibodies  >23 publications with ALCAM and Muscle
 Lung Antibodies  >22 publications with ALCAM and Lung
 Liver Antibodies  >18 publications with ALCAM and Liver
 Eye Antibodies  >17 publications with ALCAM and Eye
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