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ADORA2A elisa kit :: Mouse adenosine A2a receptor ELISA Kit

Scan QR to view Datasheet Catalog #    MBS934525
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
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 Product Name   

adenosine A2a receptor (ADORA2A), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Adenosine receptor A2a, ADORA2A ELISA Kit

 Product Synonym Names    Mouse Adenosine receptor A2a (ADORA2A) ELISA kit; ADORA2; RDC8; hA2aR; adenosine A2 receptor; adenosine receptor subtype A2a; adenosine A2a receptor
 Product Gene Name   

ADORA2A elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS934525 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q60613
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse ADORA2A. No significant cross-reactivity or interference between mouse ADORA2A and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    31.25 pg/ml -2000 pg/ml.
 Sensitivity    The minimum detectable dose of mouse ADORA2A is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ADORA2A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ADORA2A purchase    MBS934525 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the adenosine A2a receptor (ADORA2A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25 pg/ml -2000 pg/ml in biological research samples containing ADORA2A, with an estimated sensitivity of The minimum detectable dose of mouse ADORA2A is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS934525 kit is based on ADORA2A antibody-ADORA2A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADORA2A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADORA2A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ADORA2A elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADORA2A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADORA2A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADORA2A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADORA2A bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of ADORA2A elisa kit    ADORA2A elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS934525. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ADORA2A. It may not necessarily be applicable to this product.
 NCBI GI #    126517500
 NCBI GeneID    11540
 NCBI Accession #    NP_033760.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_009630.3 [Other Products]
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 UniProt Primary Accession #    Q60613 [Other Products]
 UniProt Secondary Accession #    Q2NLC1 [Other Products]
 UniProt Related Accession #    Q60613 [Other Products]
 Molecular Weight    44,971 Da
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 NCBI Official Full Name    adenosine receptor A2a
 NCBI Official Synonym Full Names    adenosine A2a receptor
 NCBI Official Symbol    Adora2a [Similar Products]
 NCBI Official Synonym Symbols   
A2aR; A2AAR; AA2AR
[Similar Products]
 NCBI Protein Information    adenosine receptor A2a
 UniProt Protein Name    Adenosine receptor A2a
 Protein Family    Adenosine receptor
 UniProt Gene Name    Adora2a [Similar Products]
 UniProt Entry Name    AA2AR_MOUSE
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 UniProt Comments for ADORA2A    ADORA2A: one of several receptor subtypes for adenosine. A G-protein coupled receptor. Activation is mediated by G proteins which activate adenylyl cyclase. Abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1

Cellular Component: integral to plasma membrane; postsynaptic density; dendrite; integral to membrane; intermediate filament; endomembrane system; axolemma; presynaptic membrane; asymmetric synapse; postsynaptic membrane; membrane; cell soma; axon; presynaptic active zone; plasma membrane

Molecular Function: G-protein coupled receptor activity; identical protein binding; signal transducer activity; enzyme binding; protein heterodimerization activity; type 5 metabotropic glutamate receptor binding; alpha-actinin binding; adenosine receptor activity, G-protein coupled

Biological Process: response to alkaloid; positive regulation of circadian sleep/wake cycle, sleep; synaptic transmission, dopaminergic; prepulse inhibition; positive regulation of glutamate secretion; regulation of synaptic plasticity; locomotory behavior; negative regulation of caspase activity; signal transduction; vasodilation; G-protein signaling, adenylate cyclase activating pathway; positive regulation of synaptic transmission, glutamatergic; synaptic transmission, cholinergic; response to caffeine; regulation of inhibitory postsynaptic membrane potential; negative regulation of cell proliferation; negative regulation of alpha-beta T cell activation; negative regulation of locomotion; regulation of transcription, DNA-dependent; regulation of mitochondrial membrane potential; positive regulation of cAMP biosynthetic process; protein kinase C activation; negative regulation of neuron apoptosis; adenosine receptor signaling pathway; positive regulation of acetylcholine secretion; synaptic transmission, glutamatergic; eating behavior; response to amphetamine; positive regulation of synaptic transmission, GABAergic; regulation of calcium ion transport; regulation of norepinephrine secretion; membrane depolarization; G-protein coupled receptor protein signaling pathway; positive regulation of protein secretion; negative regulation of inflammatory response; negative regulation of vascular permeability; negative regulation of protein kinase activity; regulation of excitatory postsynaptic membrane potential; astrocyte activation; neurite morphogenesis
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 Research Articles on ADORA2A    1. Inflammasome activity is sustained, after initial activation, by A(2A) receptor-mediated signalling.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with ADORA2A elisa kitDiseases associated with ADORA2A elisa kit
 Products by Pathway  Pathway Diagram
 Activation Of TRKA Receptors Pathway antibodies  Activation Of TRKA Receptors Pathway Diagram
 Adenosine P1 Receptors Pathway antibodies  Adenosine P1 Receptors Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (s) Signalling Events Pathway antibodies  G Alpha (s) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Disease Name  Pubmed Publications
 Genetic Predisposition to Disease Antibodies  >14 publications with ADORA2A and Genetic Predisposition to Disease
 Mental Disorders Antibodies  >12 publications with ADORA2A and Mental Disorders
 Nervous System Diseases Antibodies  >10 publications with ADORA2A and Nervous System Diseases
 Brain Diseases Antibodies  >8 publications with ADORA2A and Brain Diseases
 Inflammation Antibodies  >7 publications with ADORA2A and Inflammation
 Anxiety Disorders Antibodies  >6 publications with ADORA2A and Anxiety Disorders
 Disease Models, Animal Antibodies  >6 publications with ADORA2A and Disease Models, Animal
 Movement Disorders Antibodies  >5 publications with ADORA2A and Movement Disorders
 Basal Ganglia Diseases Antibodies  >5 publications with ADORA2A and Basal Ganglia Diseases
 Panic Disorder Antibodies  >4 publications with ADORA2A and Panic Disorder
Organs/Tissues associated with ADORA2A elisa kit
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >13 publications with ADORA2A and Brain
 Eye Antibodies  >4 publications with ADORA2A and Eye
 Bone Marrow Antibodies  >1 publications with ADORA2A and Bone Marrow
 Joint Antibodies  >1 publications with ADORA2A and Joint
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