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ADORA2B elisa kit :: Mouse adenosine A2b receptor ELISA Kit

Scan QR to view Datasheet Catalog #    MBS903160
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

adenosine A2b receptor (ADORA2B), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Adenosine receptor A2b, ADORA2B ELISA Kit

 Product Synonym Names    Mouse Adenosine receptor A2b (ADORA2B) ELISA kit; ADORA2; ; adenosine A2b receptor
 Product Gene Name   

ADORA2B elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS903160 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q60614
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Mouse ADORA2B. No significant cross-reactivity or interference between Mouse ADORA2B and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, cell culture supernates
 Assay Type    Sandwich
 Detection Range    31.25 pg/ml-2000 pg/ml
 Sensitivity    7.81 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ADORA2B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ADORA2B purchase    MBS903160 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the adenosine A2b receptor (ADORA2B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25 pg/ml-2000 pg/ml in biological research samples containing ADORA2B, with an estimated sensitivity of 7.81 pg/ml. The ELISA analytical biochemical technique of the MBS903160 kit is based on ADORA2B antibody-ADORA2B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADORA2B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADORA2B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell culture supernates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ADORA2B elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADORA2B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADORA2B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADORA2B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADORA2B bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of ADORA2B elisa kit    ADORA2B elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS903160. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ADORA2B. It may not necessarily be applicable to this product.
 NCBI GI #    31560741
 NCBI GeneID    11541
 NCBI Accession #    NP_031439.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_007413.4 [Other Products]
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 UniProt Primary Accession #    Q60614 [Other Products]
 UniProt Secondary Accession #    Q8BXI2 [Other Products]
 UniProt Related Accession #    Q60614 [Other Products]
 Molecular Weight    36,120 Da
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 NCBI Official Full Name    adenosine receptor A2b
 NCBI Official Synonym Full Names    adenosine A2b receptor
 NCBI Official Symbol    Adora2b [Similar Products]
 NCBI Official Synonym Symbols   
A2b; A2BR; A2BAR; AA2BR; AI480866; AI605384
[Similar Products]
 NCBI Protein Information    adenosine receptor A2b; A2b, Rs
 UniProt Protein Name    Adenosine receptor A2b
 Protein Family    Adenosine receptor
 UniProt Gene Name    Adora2b [Similar Products]
 UniProt Entry Name    AA2BR_MOUSE
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 UniProt Comments for ADORA2B    ADORA2B: Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass

Cellular Component: cell surface; membrane; integral to membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; signal transducer activity; adenosine receptor activity, G-protein coupled

Biological Process: relaxation of vascular smooth muscle; negative regulation of collagen biosynthetic process; positive regulation of interleukin-6 production; positive regulation of guanylate cyclase activity; positive regulation of mast cell degranulation; positive regulation of vasodilation; signal transduction; G-protein signaling, adenylate cyclase activating pathway; regulation of angiogenesis; positive regulation of chemokine production; G-protein coupled receptor protein signaling pathway; negative regulation of cell proliferation; positive regulation of cGMP biosynthetic process; positive regulation of chronic inflammatory response to non-antigenic stimulus; positive regulation of cAMP biosynthetic process; positive regulation of cell proliferation; cellular response to extracellular stimulus; positive regulation of catecholamine secretion; adenosine receptor signaling pathway
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 Research Articles on ADORA2B    1. CD73-dependent production of extracellular adenosine and endothelial Adora2b signaling protects kidney during diabetic nephropathy.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with ADORA2B elisa kitDiseases associated with ADORA2B elisa kit
 Products by Pathway  Pathway Diagram
 Adenosine P1 Receptors Pathway antibodies  Adenosine P1 Receptors Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (s) Signalling Events Pathway antibodies  G Alpha (s) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 GPCRs, Class A Rhodopsin-like Pathway antibodies  GPCRs, Class A Rhodopsin-like Pathway Diagram
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >7 publications with ADORA2B and Inflammation
 Nervous System Diseases Antibodies  >5 publications with ADORA2B and Nervous System Diseases
 Myocardial Ischemia Antibodies  >4 publications with ADORA2B and Myocardial Ischemia
 Intestinal Diseases Antibodies  >3 publications with ADORA2B and Intestinal Diseases
 Cardiovascular Diseases Antibodies  >2 publications with ADORA2B and Cardiovascular Diseases
 Neoplasms Antibodies  >2 publications with ADORA2B and Neoplasms
 Disease Models, Animal Antibodies  >2 publications with ADORA2B and Disease Models, Animal
 Immune System Diseases Antibodies  >2 publications with ADORA2B and Immune System Diseases
 Heart Diseases Antibodies  >1 publications with ADORA2B and Heart Diseases
 Adenocarcinoma Antibodies  >1 publications with ADORA2B and Adenocarcinoma
Organs/Tissues associated with ADORA2B elisa kit
 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >3 publications with ADORA2B and Eye
 Brain Antibodies  >3 publications with ADORA2B and Brain
 Lung Antibodies  >2 publications with ADORA2B and Lung
 Intestine Antibodies  >2 publications with ADORA2B and Intestine
 Bone Marrow Antibodies  >1 publications with ADORA2B and Bone Marrow
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