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AMPD2 elisa kit :: Mouse adenosine monophosphate deaminase 2 (isoform L) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS913798
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
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 Product Name   

adenosine monophosphate deaminase 2 (isoform L) (AMPD2), ELISA Kit

★Popular Item★
 Also Known As   

Mouse AMP deaminase 2, AMPD2 ELISA Kit

 Product Synonym Names    Mouse AMP deaminase 2 (AMPD2) ELISA kit; AMP deaminase 2; OTTHUMP00000013371; adenosine monophosphate deaminase 2 (isoform L)
 Product Gene Name   

AMPD2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS913798 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q9DBT5
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse AMPD2. No significant cross-reactivity or interference between mouse AMPD2 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    28 pg/ml -1800 pg/ml.
 Sensitivity    The minimum detectable dose of mouse AMPD2 is typically less than 7 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of AMPD2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for AMPD2 purchase    MBS913798 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the adenosine monophosphate deaminase 2 (isoform L) (AMPD2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 28 pg/ml -1800 pg/ml in biological research samples containing AMPD2, with an estimated sensitivity of The minimum detectable dose of mouse AMPD2 is typically less than 7 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS913798 kit is based on AMPD2 antibody-AMPD2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AMPD2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AMPD2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for AMPD2 elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AMPD2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AMPD2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AMPD2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AMPD2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of AMPD2 elisa kit    AMPD2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS913798. Click to request current manual
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NCBI/Uniprot data below describe general gene information for AMPD2. It may not necessarily be applicable to this product.
 NCBI GI #    576067830
 NCBI GeneID    109674
 NCBI Accession #    NP_001276648.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001289719.1 [Other Products]
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 UniProt Primary Accession #    Q9DBT5 [Other Products]
 UniProt Secondary Accession #    Q91YI2; A2AE28 [Other Products]
 UniProt Related Accession #    Q9DBT5 [Other Products]
 Molecular Weight    92,024 Da
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 NCBI Official Full Name    AMP deaminase 2 isoform b
 NCBI Official Synonym Full Names    adenosine monophosphate deaminase 2
 NCBI Official Symbol    Ampd2 [Similar Products]
 NCBI Official Synonym Symbols   
Ampd-2; AI552571; 1200014F01Rik
[Similar Products]
 NCBI Protein Information    AMP deaminase 2; adenosine monophosphate deaminase 2 (isoform L)
 UniProt Protein Name    AMP deaminase 2
 UniProt Synonym Protein Names   
AMP deaminase isoform L
 Protein Family    AMP deaminase
 UniProt Gene Name    Ampd2 [Similar Products]
 UniProt Entry Name    AMPD2_MOUSE
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 UniProt Comments for AMPD2    AMPD2: AMP deaminase plays a critical role in energy metabolism. Belongs to the adenosine and AMP deaminases family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleotide Metabolism - purine; Hydrolase; EC 3.5.4.6

Molecular Function: hydrolase activity; metal ion binding; deaminase activity; AMP deaminase activity

Biological Process: IMP biosynthetic process; purine ribonucleoside monophosphate biosynthetic process; IMP salvage; nucleotide metabolic process
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with AMPD2 elisa kitDiseases associated with AMPD2 elisa kit
 Products by Pathway  Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Nucleotides Pathway antibodies  Metabolism Of Nucleotides Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Salvage Pathway antibodies  Purine Salvage Pathway Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >3 publications with AMPD2 and Neoplasms
 Proteinuria Antibodies  >1 publications with AMPD2 and Proteinuria
 Adenocarcinoma Antibodies  >1 publications with AMPD2 and Adenocarcinoma
 Inflammation Antibodies  >1 publications with AMPD2 and Inflammation
 Liver Diseases Antibodies  >1 publications with AMPD2 and Liver Diseases
 Carcinoma, Hepatocellular Antibodies  >1 publications with AMPD2 and Carcinoma, Hepatocellular
 Liver Neoplasms Antibodies  >1 publications with AMPD2 and Liver Neoplasms
 Liver Neoplasms, Experimental Antibodies  >1 publications with AMPD2 and Liver Neoplasms, Experimental
Organs/Tissues associated with AMPD2 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >7 publications with AMPD2 and Liver
 Kidney Antibodies  >3 publications with AMPD2 and Kidney
 Brain Antibodies  >3 publications with AMPD2 and Brain
 Embryonic Tissue Antibodies  >2 publications with AMPD2 and Embryonic Tissue
 Lung Antibodies  >1 publications with AMPD2 and Lung
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