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ANGPT2 elisa kit :: Mouse angiopoietin 2 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS702253
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $450 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,615 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,940 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

angiopoietin 2 (ANGPT2), ELISA Kit

 Also Known As   

Mouse Angiopoietin 2, ANG-2 ELISA Kit

 Product Synonym Names    Mouse Angiopoietin 2; ANG-2 ELISA Kit; AGPT2; ANG2; Tie2-ligand; angiopoietin-2; angiopoietin-2B; angiopoietin-2a; angiopoietin 2
 Product Gene Name   

ANGPT2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS702253 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for O35608
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Mouse ANGPT2. No significant cross-reactivity or interference between Mouse ANGPT2 and analogues was observed.
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 Samples    Serum, plasma, cell culture supernates, tissue homogenates
 Detection Range    0.156 pg/ml-10 pg/ml
 Sensitivity    0.039 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Angiogenesis
 Protein Biological Process 3    Angiogenesis
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ANGPT2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ANGPT2 purchase    MBS702253 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the angiopoietin 2 (ANGPT2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156 pg/ml-10 pg/ml in biological research samples containing ANGPT2, with an estimated sensitivity of 0.039 pg/ml. The ELISA analytical biochemical technique of the MBS702253 kit is based on ANGPT2 antibody-ANGPT2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ANGPT2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ANGPT2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernates, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ANGPT2 elisa kit

   Introduction: The angiopoietins are protein growth factors that promote angiogenesis, the formation of blood vessels from pre-existing blood vessels. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The mechanism by which they contribute to angiogenesis is thought to involve regulation of endothelial cell interactions with supporting perivascular cells. There are now four identified angiopoietins: Ang-1, Ang-2, Ang-3, Ang-4. Human and mouse Ang-2 share 85% amino acid identity and Ang-2 is approximately 60% identical to Ang-1. Ang-2 contains an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain. Located at the N-terminus are stretches of hydrophobic residues typical of secretory signal sequences. Although likely playing different functional roles, both Ang-1 and Ang-2 are ligands for the endothelial cell receptor tyrosine kinase Tie-2. The regulation of Tie-2 activity by Ang-2 is complex, leading to inhibition in certain cell types or conditions, and activation in others. In vitro studies demonstrated that Ang-1, but not Ang-2, could stimulate Tie-2 tyrosine phosphorylation and suggested that 2 Ang-2 could act as a competitive inhibitor of Ang-1 signaling. Consistent with its role as an Ang-1/Tie-2 inhibitor, transgenic overexpression of Ang-2 leads to disruption of embryonic blood vessel formation, a phenotype similar to that of Tie-2 knockouts. In contrast, altering incubation times or elevating Ang-2 concentrations, can under some circumstances, result in Tie-2 activation in HUVECs. It has also been proposed that Ang-2 may play a pro-angiogenic role by mediating destabilizing interactions between endothelial and perivascular cells, enhancing the effects of pro-angiogenic proteins including vascular endothelial growth factor (VEGF). Alone, Ang-2 promotes endothelial cell death and vessel regression, but can act in synergy with VEGF to promote new vessel formation. Ang-2 has been implicated in cancer development due to its role in angiogenesis. Ang-2-mediated destabilization of mature vessel structures accompanied by the presence of VEGF, may contribute to the angiogenesis associated with tumor tissues. Several studies have examined Ang-2 expression in an assortment of human cancers. Ang-2 is elevated in colon carcinoma, gastric carcinoma, glioblastoma, glioma, hepatocellular carcinoma, neuroblastoma and non-small cell lung cancer. Furthermore, Ang-2 levels appear to correlate with advancing tumor stage progression and poor prognosis.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ANG-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for ANG-2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain ANG-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ANG-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of ANGPT2 elisa kit    ANGPT2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS702253. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ANGPT2. It may not necessarily be applicable to this product.
 NCBI GI #    31982508
 NCBI GeneID    11601
 NCBI Accession #    NP_031452.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_007426.4 [Other Products]
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 UniProt Primary Accession #    O35608 [Other Products]
 UniProt Secondary Accession #    Q3U1A1; Q9D2D2 [Other Products]
 UniProt Related Accession #    O35608 [Other Products]
 Molecular Weight    56,576 Da
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 NCBI Official Full Name    angiopoietin-2
 NCBI Official Synonym Full Names    angiopoietin 2
 NCBI Official Symbol    Angpt2 [Similar Products]
 NCBI Official Synonym Symbols   
Ang2; Agpt2; Ang-2
[Similar Products]
 NCBI Protein Information    angiopoietin-2
 UniProt Protein Name    Angiopoietin-2
 Protein Family    Angiopoietin
 UniProt Gene Name    Angpt2 [Similar Products]
 UniProt Synonym Gene Names    Agpt2; ANG-2 [Similar Products]
 UniProt Entry Name    ANGP2_MOUSE
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 NCBI Summary for ANGPT2    This gene encodes an endothelial cell (EC)-derived regulator of angiogenesis and ligand for endothelial-specific receptor tyrosine kinase. The encoded protein acts as an anti-apoptotic factor for stressed ECs and a proapoptotic factor for resting ECs. [provided by RefSeq, Jan 2013]
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 UniProt Comments for ANGPT2    Function: Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal
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 Research Articles on ANGPT2    1. This study identifies angiopoietin-2 as a link between kidney fibrosis and arterial stiffness.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ANGPT2 elisa kitPathways associated with ANGPT2 elisa kit
 Reference Product  PubMed Publications
 ANGPT1 elisa kit  >704 publications with ANGPT2 and ANGPT1
 TEK elisa kit  >29 publications with ANGPT2 and TEK
 Products by Pathway  Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 HIF-1 Signaling Pathway antibodies  HIF-1 Signaling Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 PI3K-Akt Signaling Pathway antibodies  PI3K-Akt Signaling Pathway Diagram
 PI3K-Akt Signaling Pathway antibodies  PI3K-Akt Signaling Pathway Diagram
 Rap1 Signaling Pathway antibodies  Rap1 Signaling Pathway Diagram
 Rap1 Signaling Pathway antibodies  Rap1 Signaling Pathway Diagram
 Ras Signaling Pathway antibodies  Ras Signaling Pathway Diagram
 Tie2 Signaling Pathway antibodies  Tie2 Signaling Pathway Diagram
Diseases associated with ANGPT2 elisa kitOrgans/Tissues associated with ANGPT2 elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >256 publications with ANGPT2 and Cardiovascular Diseases
 Inflammation Antibodies  >214 publications with ANGPT2 and Inflammation
 Disease Models, Animal Antibodies  >160 publications with ANGPT2 and Disease Models, Animal
 Necrosis Antibodies  >112 publications with ANGPT2 and Necrosis
 Liver Diseases Antibodies  >82 publications with ANGPT2 and Liver Diseases
 Neoplasms, Experimental Antibodies  >79 publications with ANGPT2 and Neoplasms, Experimental
 Heart Diseases Antibodies  >77 publications with ANGPT2 and Heart Diseases
 Liver Neoplasms Antibodies  >75 publications with ANGPT2 and Liver Neoplasms
 Hypertension Antibodies  >71 publications with ANGPT2 and Hypertension
 Kidney Diseases Antibodies  >68 publications with ANGPT2 and Kidney Diseases
 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >1034 publications with ANGPT2 and Vascular
 Muscle Antibodies  >145 publications with ANGPT2 and Muscle
 Embryonic Tissue Antibodies  >123 publications with ANGPT2 and Embryonic Tissue
 Brain Antibodies  >110 publications with ANGPT2 and Brain
 Lung Antibodies  >97 publications with ANGPT2 and Lung
 Liver Antibodies  >92 publications with ANGPT2 and Liver
 Eye Antibodies  >71 publications with ANGPT2 and Eye
 Kidney Antibodies  >68 publications with ANGPT2 and Kidney
 Placenta Antibodies  >58 publications with ANGPT2 and Placenta
 Bone Marrow Antibodies  >46 publications with ANGPT2 and Bone Marrow
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