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| Product Name
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angiopoietin-like 4 (ANGPTL4), ELISA Kit ★Popular Item★
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| Also Known As
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Mouse Angiopoietin-related protein 4, ANGPTL4 ELISA Kit
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| Product Synonym Names
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Mouse Angiopoietin-related protein 4 (ANGPTL4) ELISA kit; ANGPTL2; ARP4; FIAF; HFARP; NL2; PGAR; pp1158; PPARG angiopoietin related protein; angiopoietin-like 4 protein; fasting-induced adipose factor; hepatic angiopoietin-related protein; hepatic fibrinogen/a; angiopoietin-like 4
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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| Sample Manual Insert
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| Request for Current Manual Insert
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| MBS915491 Testing Data
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| 3D Structure
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ModBase 3D Structure for Q9Z1P8
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| Species Reactivity
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Mouse
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| Specificity
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This assay has high sensitivity and excellent specificity for detection of mouse ANGPTL4. No significant cross-reactivity or interference between mouse ANGPTL4 and analogues was observed.
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| Samples
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Serum, plasma, tissue homogenates.
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| Assay Type
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Competitive
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| Detection Range
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3.125 pg/ml-200 pg/ml.
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| Sensitivity
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0.781 pg/ml.
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| Intra-assay Precision
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Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
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| Inter-assay Precision
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Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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| Preparation and Storage
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Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
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| ISO Certification
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Manufactured in an ISO 9001:2008 Certified Laboratory.
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| Product Note
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Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
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| Other Notes
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Small volumes of ANGPTL4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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| Searchable Terms for ANGPTL4 purchase
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MBS915491 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the angiopoietin-like 4 (ANGPTL4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 3.125 pg/ml-200 pg/ml in biological research samples containing ANGPTL4, with an estimated sensitivity of 0.781 pg/ml. The ELISA analytical biochemical technique of the MBS915491 kit is based on ANGPTL4 antibody-ANGPTL4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ANGPTL4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ANGPTL4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with ANGPTL4. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for ANGPTL4. The competitive inhibition reaction is launched between with pre-coated ANGPTL4 and ANGPTL4 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of ANGPTL4 in the samples. The color development is stopped and the intensity of the color is measured.
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| Typical Testing Data/Standard Curve (for reference only) of ANGPTL4 elisa kit
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| Sample Manual Insert of MBS915491. Click to request current manual
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| NCBI/Uniprot data below describe general gene information for ANGPTL4. It may not necessarily be applicable to this product. |
| NCBI GI #
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255308873
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| NCBI GeneID
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57875
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| NCBI Accession #
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NP_065606.2
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[Other Products]
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| NCBI GenBank Nucleotide #
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NM_020581.2
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[Other Products]
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| UniProt Primary Accession #
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Q9Z1P8
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[Other Products]
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| UniProt Secondary Accession #
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Q78ZJ9; Q9JHX7; Q9JLX7
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[Other Products]
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| UniProt Related Accession #
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Q9Z1P8
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[Other Products]
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| Molecular Weight
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45,538 Da
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| NCBI Official Full Name
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angiopoietin-related protein 4
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| NCBI Official Synonym Full Names
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angiopoietin-like 4
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| NCBI Official Symbol
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Angptl4
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[Similar Products]
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| NCBI Official Synonym Symbols
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Arp4; Bk89; Fiaf; Ng27; Pgar; Hfarp; Pgarg; Pp1158
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[Similar Products]
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| NCBI Protein Information
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angiopoietin-related protein 4; 425O18-1; secreted protein Bk89; angiopoietin-like protein 4; fasting-induced adipose factor; fibrinogen/angiopoietin-related protein; major histocompatibility complex region NG27; hepatic fibrinogen/angiopoietin-related protein
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| UniProt Protein Name
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Angiopoietin-related protein 4
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| UniProt Synonym Protein Names
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425O18-1; Angiopoietin-like protein 4; Fasting-induced adipose factor; Hepatic fibrinogen/angiopoietin-related protein; HFARP; Secreted protein Bk89
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| Protein Family
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Angiopoietin-related protein
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| UniProt Gene Name
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Angptl4
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[Similar Products]
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| UniProt Synonym Gene Names
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Farp; Fiaf; Ng27; HFARP
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[Similar Products]
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| UniProt Entry Name
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ANGL4_MOUSE
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| UniProt Comments for ANGPTL4
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ANGPTL4: Protein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorigenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation.
Protein type: Secreted; Apoptosis; Extracellular matrix; Inhibitor; Secreted, signal peptide
Cellular Component: proteinaceous extracellular matrix; extracellular space; extracellular region
Molecular Function: enzyme inhibitor activity
Biological Process: positive regulation of lipid metabolic process; negative regulation of lipoprotein lipase activity; angiogenesis; cellular response to starvation; protein homooligomerization; negative regulation of apoptosis
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| Research Articles on ANGPTL4
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1. ANGPTL4 is a genetically and epigenetically inactivated secreted tumor suppressor that inhibits tumor angiogenesis.
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Pathways associated with ANGPTL4 elisa kit | Diseases associated with ANGPTL4 elisa kit |
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| Organs/Tissues associated with ANGPTL4 elisa kit |
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ELISA Kit 
