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ANXA1 elisa kit :: Mouse annexin A1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS944251
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

annexin A1 (ANXA1), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Annexin A1, ANXA1 ELISA Kit

 Product Synonym Names    Mouse Annexin A1 (ANXA1) ELISA kit; ANX1; LPC1; OTTHUMP00000021475; annexin I; annexin I (lipocortin I) ; lipocortin I; annexin A1
 Product Gene Name   

ANXA1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS944251 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P10107
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse ANXA1. No significant cross-reactivity or interference between mouse ANXA1 and analogues was observed.
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 Samples    Serum, plasma, cell culture supernates, tissue homogenates.
 Assay Type    Sandwich
 Detection Range    0.625 ng/ml-40 ng/ml.
 Sensitivity    Typically less than 0.156 ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ANXA1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ANXA1 purchase    MBS944251 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the annexin A1 (ANXA1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.625 ng/ml-40 ng/ml in biological research samples containing ANXA1, with an estimated sensitivity of Typically less than 0.156 ng/ml. The ELISA analytical biochemical technique of the MBS944251 kit is based on ANXA1 antibody-ANXA1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ANXA1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ANXA1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernates, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ANXA1 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ANXA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ANXA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ANXA1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ANXA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of ANXA1 elisa kit    ANXA1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS944251. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ANXA1. It may not necessarily be applicable to this product.
 NCBI GI #    124517663
 NCBI GeneID    16952
 NCBI Accession #    NP_034860.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_010730.2 [Other Products]
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 UniProt Primary Accession #    P10107 [Other Products]
 UniProt Related Accession #    P10107 [Other Products]
 Molecular Weight    38,734 Da
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 NCBI Official Full Name    annexin A1
 NCBI Official Synonym Full Names    annexin A1
 NCBI Official Symbol    Anxa1 [Similar Products]
 NCBI Official Synonym Symbols   
Lpc1; Anx-1; Lpc-1; Anx-A1; C430014K04Rik
[Similar Products]
 NCBI Protein Information    annexin A1; p35; annexin I; annexin-1; calpactin-2; calpactin II; lipocortin 1; lipocortin I; chromobindin-9; phospholipase A2 inhibitory protein
 UniProt Protein Name    Annexin A1
 UniProt Synonym Protein Names   
Annexin I; Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; Lipocortin I; Phospholipase A2 inhibitory protein; p35
 Protein Family    Annexin
 UniProt Gene Name    Anxa1 [Similar Products]
 UniProt Synonym Gene Names    Anx1; Lpc-1; Lpc1 [Similar Products]
 UniProt Entry Name    ANXA1_MOUSE
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 UniProt Comments for ANXA1    ANXA1: a calcium/phospholipid-binding protein with which promotes membrane fusion and is involved in endocytosis. Has anti-inflammatory properties and inhibits phospholipase A2 activity. Accumulates on internalized vesicles after EGF-stimulated endocytosis, suggesting that it may be required for a late stage in inward vesiculation. Phosphorylated by PKC, EGFR and Chak1. Phosphorylation results in loss of the inhibitory activity. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.

Protein type: Lipid-binding; Calcium-binding

Cellular Component: extracellular space; focal adhesion; protein complex; mast cell granule; cell surface; cornified envelope; cilium; cell projection; membrane; cytoplasm; mitochondrial membrane; plasma membrane; nucleus; vesicle; endosome; sarcolemma

Molecular Function: protein binding, bridging; phospholipase inhibitor activity; protein binding; protein homodimerization activity; single-stranded RNA binding; calcium-dependent phospholipid binding; phospholipase A2 inhibitor activity; double-stranded DNA-dependent ATPase activity; phospholipid binding; calcium ion binding; structural molecule activity; calcium-dependent protein binding; helicase activity; single-stranded DNA binding

Biological Process: negative regulation of protein secretion; positive regulation of neutrophil apoptosis; positive regulation of apoptosis; alpha-beta T cell differentiation; positive regulation of vesicle fusion; neutrophil homeostasis; signal transduction; arachidonic acid secretion; DNA duplex unwinding; regulation of cell proliferation; keratinocyte differentiation; cell surface receptor linked signal transduction; insulin secretion; DNA strand renaturation; peptide cross-linking; positive regulation of prostaglandin biosynthetic process
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 Research Articles on ANXA1    1. Data show an increase of extracellular Annexin A1 (ANXA1) starting from the initial phases of skeletal muscle cell differentiation.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with ANXA1 elisa kitDiseases associated with ANXA1 elisa kit
 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Formyl Peptide Receptors Bind Formyl Peptides And Many Other Ligands Pathway antibodies  Formyl Peptide Receptors Bind Formyl Peptides And Many Other Ligands Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Peptide Ligand-binding Receptors Pathway antibodies  Peptide Ligand-binding Receptors Pathway Diagram
 Prostaglandin Synthesis And Regulation Pathway antibodies  Prostaglandin Synthesis And Regulation Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By GPCR Pathway antibodies  Signaling By GPCR Pathway Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >73 publications with ANXA1 and Neoplasms
 Inflammation Antibodies  >44 publications with ANXA1 and Inflammation
 Carcinoma Antibodies  >42 publications with ANXA1 and Carcinoma
 Nervous System Diseases Antibodies  >17 publications with ANXA1 and Nervous System Diseases
 Neoplasm Metastasis Antibodies  >14 publications with ANXA1 and Neoplasm Metastasis
 Breast Neoplasms Antibodies  >14 publications with ANXA1 and Breast Neoplasms
 Gastrointestinal Diseases Antibodies  >12 publications with ANXA1 and Gastrointestinal Diseases
 Lung Diseases Antibodies  >10 publications with ANXA1 and Lung Diseases
 Necrosis Antibodies  >9 publications with ANXA1 and Necrosis
 Brain Diseases Antibodies  >9 publications with ANXA1 and Brain Diseases
Organs/Tissues associated with ANXA1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >57 publications with ANXA1 and Blood
 Brain Antibodies  >18 publications with ANXA1 and Brain
 Lung Antibodies  >15 publications with ANXA1 and Lung
 Pituitary Gland Antibodies  >12 publications with ANXA1 and Pituitary Gland
 Liver Antibodies  >8 publications with ANXA1 and Liver
 Muscle Antibodies  >7 publications with ANXA1 and Muscle
 Bone Antibodies  >7 publications with ANXA1 and Bone
 Kidney Antibodies  >5 publications with ANXA1 and Kidney
 Stomach Antibodies  >5 publications with ANXA1 and Stomach
 Bone Marrow Antibodies  >5 publications with ANXA1 and Bone Marrow
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