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CPS1 elisa kit :: Mouse carbamoyl-phosphate synthetase 1, mitochondrial ELISA Kit

Scan QR to view Datasheet Catalog #    MBS920676
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

carbamoyl-phosphate synthetase 1, mitochondrial (CPS1), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Carbamoyl-phosphate synthase [ammonia], mitochondrial, CPS1 ELISA Kit

 Product Synonym Names    Mouse Carbamoyl-phosphate synthase [ammonia]; mitochondrial (CPS1) ELISA kit; CPSase I; carbamoyl-phosphate synthetase 1; carbamoylphosphate synthetase I; carbamoyl-phosphate synthetase 1; mitochondrial
 Product Gene Name   

CPS1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS920676 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q8C196
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Mouse CPS1. No significant cross-reactivity or interference between Mouse CPS1 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    6.25 pg/ml-400 pg/ml
 Sensitivity    1.56 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Biosynthesis/Metabolism
 Protein Biological Process 3    Urea cycle
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CPS1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CPS1 purchase    MBS920676 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the carbamoyl-phosphate synthetase 1, mitochondrial (CPS1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 6.25 pg/ml-400 pg/ml in biological research samples containing CPS1, with an estimated sensitivity of 1.56 pg/ml. The ELISA analytical biochemical technique of the MBS920676 kit is based on CPS1 antibody-CPS1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CPS1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CPS1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CPS1 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CPS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CPS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CPS1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CPS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of CPS1 elisa kit    CPS1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS920676. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CPS1. It may not necessarily be applicable to this product.
 NCBI GI #    124248512
 NCBI GeneID    227231
 NCBI Accession #    NP_001074278.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001080809.2 [Other Products]
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 UniProt Primary Accession #    Q8C196 [Other Products]
 UniProt Secondary Accession #    Q6NX75; A0JNU4 [Other Products]
 UniProt Related Accession #    Q8C196 [Other Products]
 Molecular Weight    164,618 Da
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 NCBI Official Full Name    carbamoyl-phosphate synthase
 NCBI Official Synonym Full Names    carbamoyl-phosphate synthetase 1
 NCBI Official Symbol    Cps1 [Similar Products]
 NCBI Official Synonym Symbols   
CPS; D1Ucla3; 4732433M03Rik
[Similar Products]
 NCBI Protein Information    carbamoyl-phosphate synthase [ammonia], mitochondrial; carbamoyl-phosphate synthase [ammonia], mitochondrial; CPSase I; carbamoylphosphate synthetase I; carbamoyl-phosphate synthetase I
 UniProt Protein Name    Carbamoyl-phosphate synthase [ammonia], mitochondrial
 UniProt Synonym Protein Names   
Carbamoyl-phosphate synthetase I; CPSase I
 Protein Family    Carboxypeptidase
 UniProt Gene Name    Cps1 [Similar Products]
 UniProt Synonym Gene Names    CPSase I [Similar Products]
 UniProt Entry Name    CPSM_MOUSE
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 NCBI Summary for CPS1    This gene encodes a protein localized to the inner mitochondrial matrix. The encoded protein plays a role in the detoxification of ammonia by catalyzing the first step in the urea cycle in which carbomyl-phosphate is synthesized from ammonia and bicarbonate. Carbamoyl-phosphate is subsequently converted to urea that is excreted by the kidneys. Deficiency of the encoded enzyme leads to an accumulation of ammonia in the blood. High levels of ammonia are toxic to the central nervous system and result in neurological disorders. [provided by RefSeq, Oct 2013]
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 UniProt Comments for CPS1    CPS1: Involved in the urea cycle of ureotelic animals where the enzyme plays an important role in removing excess ammonia from the cell. Defects in CPS1 are the cause of carbamoyl phosphate synthetase 1 deficiency (CPS1D). CPS1D is an autosomal recessive disorder of the urea cycle causing hyperammonemia. Clinical features include protein intolerance, intermittent ataxia, seizures, lethargy, developmental delay and mental retardation. Genetic variations in CPS1 influence the availability of precursors for nitric oxide (NO) synthesis and play a role in clinical situations where endogenous NO production is critically important, such as neonatal pulmonary hypertension, increased pulmonary artery pressure following surgical repair of congenital heart defects or hepatovenocclusive disease following bone marrow transplantation. Infants with neonatal pulmonary hypertension homozygous for Thr-1406 have lower L-arginine concentrations than neonates homozygous for Asn-1406. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Mitochondrial; EC 6.3.4.16; Ligase; Energy Metabolism - nitrogen; Amino Acid Metabolism - arginine and proline; Nucleolus

Cellular Component: protein complex; mitochondrion; cytoplasm; mitochondrial inner membrane; nucleus

Molecular Function: carbamoyl-phosphate synthase (ammonia) activity; protein binding; glutamate binding; carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity; metal ion binding; endopeptidase activity; nucleotide binding; protein complex binding; phospholipid binding; calcium ion binding; catalytic activity; ATP binding; ligase activity

Biological Process: nitrogen compound metabolic process; glycogen catabolic process; arginine biosynthetic process; metabolic process; glutamine catabolic process; triacylglycerol catabolic process; response to lipopolysaccharide; homocysteine metabolic process; positive regulation of vasodilation; proteolysis; nitric oxide metabolic process; anion homeostasis; urea cycle
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 Research Articles on CPS1    1. Taken together with previous findings regarding CPS1 structure and function, homology modeling of mouse CPS1 suggested that nitration at Y1450 in an alpha-helix of allosteric domain prevents activation of CPS1 by its activator, N-acetyl-l-glutamate.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with CPS1 elisa kitDiseases associated with CPS1 elisa kit
 Products by Pathway  Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Amino Acids And Derivatives Pathway antibodies  Metabolism Of Amino Acids And Derivatives Pathway Diagram
 Urea Cycle Pathway antibodies  Urea Cycle Pathway Diagram
 Urea Cycle Pathway antibodies  Urea Cycle Pathway Diagram
 Urea Cycle Pathway antibodies  Urea Cycle Pathway Diagram
 Urea Cycle And Metabolism Of Amino Groups Pathway antibodies  Urea Cycle And Metabolism Of Amino Groups Pathway Diagram
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >18 publications with CPS1 and Nervous System Diseases
 Brain Diseases Antibodies  >18 publications with CPS1 and Brain Diseases
 Hyperammonemia Antibodies  >16 publications with CPS1 and Hyperammonemia
 Carbamoyl-Phosphate Synthase I Deficiency Disease Antibodies  >13 publications with CPS1 and Carbamoyl-Phosphate Synthase I Deficiency Disease
 Neoplasms Antibodies  >11 publications with CPS1 and Neoplasms
 Liver Diseases Antibodies  >9 publications with CPS1 and Liver Diseases
 Liver Neoplasms Antibodies  >7 publications with CPS1 and Liver Neoplasms
 Carcinoma Antibodies  >6 publications with CPS1 and Carcinoma
 Adenocarcinoma Antibodies  >4 publications with CPS1 and Adenocarcinoma
 Kidney Diseases Antibodies  >4 publications with CPS1 and Kidney Diseases
Organs/Tissues associated with CPS1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >26 publications with CPS1 and Liver
 Intestine Antibodies  >5 publications with CPS1 and Intestine
 Lung Antibodies  >3 publications with CPS1 and Lung
 Skin Antibodies  >2 publications with CPS1 and Skin
 Embryonic Tissue Antibodies  >1 publications with CPS1 and Embryonic Tissue
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