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CHST2 elisa kit :: Mouse carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS913065
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
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 Product Name   

carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2 (CHST2), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Carbohydrate sulfotransferase 2, CHST2 ELISA Kit

 Product Synonym Names    Mouse Carbohydrate sulfotransferase 2 (CHST2) ELISA kit; C6ST; GST2; N-acetylglucosamine 6-O-sulfotransferase; carbohydrate (chondroitin 6/keratan) sulfotransferase 2; carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2
 Product Gene Name   

CHST2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS913065 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q80WV3
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse CHST2. No significant cross-reactivity or interference between mouse CHST2 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    25 pg/ml-1600 pg/ml.
 Sensitivity    The minimum detectable dose of mouse CHST2 is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CHST2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CHST2 purchase    MBS913065 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2 (CHST2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 25 pg/ml-1600 pg/ml in biological research samples containing CHST2, with an estimated sensitivity of The minimum detectable dose of mouse CHST2 is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS913065 kit is based on CHST2 antibody-CHST2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CHST2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CHST2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CHST2 elisa kit

   Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CHST2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CHST2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CHST2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHST2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of CHST2 elisa kit    CHST2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS913065. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CHST2. It may not necessarily be applicable to this product.
 NCBI GI #    157266319
 NCBI GeneID    54371
 NCBI Accession #    NP_061233.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_018763.2 [Other Products]
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 UniProt Primary Accession #    Q80WV3 [Other Products]
 UniProt Secondary Accession #    O88276; Q794G9; E9QNG2 [Other Products]
 UniProt Related Accession #    Q80WV3 [Other Products]
 Molecular Weight    57,828 Da
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 NCBI Official Full Name    carbohydrate sulfotransferase 2
 NCBI Official Synonym Full Names    carbohydrate sulfotransferase 2
 NCBI Official Symbol    Chst2 [Similar Products]
 NCBI Official Synonym Symbols   
Chts2; GST-2; Gn6st; AI428561; AW121776; GlcNAc6ST; C130041E03Rik
[Similar Products]
 NCBI Protein Information    carbohydrate sulfotransferase 2; gn6st-1; glcNAc6ST-1; N-acetylglucosamine-6-O-sulfotransferase; N-acetylglucosamine 6-O-sulfotransferase 1; galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 2
 UniProt Protein Name    Carbohydrate sulfotransferase 2
 UniProt Synonym Protein Names   
Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 2; GST-2; N-acetylglucosamine 6-O-sulfotransferase 1; GlcNAc6ST-1; Gn6st-1
 Protein Family    Carbohydrate sulfotransferase
 UniProt Gene Name    Chst2 [Similar Products]
 UniProt Synonym Gene Names    Gst2; GST-2; GlcNAc6ST-1; Gn6st-1 [Similar Products]
 UniProt Entry Name    CHST2_MOUSE
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 UniProt Comments for CHST2    CHST2: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within keratan-like structures on N-linked glycans and within mucin-associated glycans that can ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X and in lymphocyte homing to Peyer patches. Has no activity toward O-linked sugars. Its substrate specificity may be influenced by its subcellular location. Sulfates GlcNAc residues at terminal, non-reducing ends of oligosaccharide chains. Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: Membrane protein, integral; Transferase; EC 2.8.2.-; Glycan Metabolism - keratan sulfate biosynthesis

Cellular Component: Golgi membrane; Golgi apparatus; membrane; integral to membrane; trans-Golgi network

Molecular Function: phenanthrol sulfotransferase activity; proteoglycan sulfotransferase activity; trans-3,4-dihydrodiolphenanthrene sulfotransferase activity; 2-phenanthrol sulfotransferase activity; galactose 3-O-sulfotransferase activity; 1-phenanthrol sulfotransferase activity; transferase activity; 3-phenanthrol sulfotransferase activity; sulfotransferase activity; HNK-1 sulfotransferase activity; N-acetylglucosamine 6-O-sulfotransferase activity; 4-phenanthrol sulfotransferase activity; heparan sulfate 2-O-sulfotransferase activity; cholesterol sulfotransferase activity; trans-9R,10R-dihydrodiolphenanthrene sulfotransferase activity; N-acetylgalactosamine 4-O-sulfotransferase activity; 9-phenanthrol sulfotransferase activity; heparan sulfate 6-O-sulfotransferase activity

Biological Process: sulfur metabolic process; N-acetylglucosamine metabolic process; carbohydrate metabolic process; inflammatory response
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 Research Articles on CHST2    1. data indicate that keratan sulfate expression is closely associated with functional disturbance after spinal cord injury; N-acetylglucosamine 6-O-sulfotransferase-1-deficient mice are a good model to investigate the roles of keratan sulfate in the CNS
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with CHST2 elisa kitDiseases associated with CHST2 elisa kit
 Products by Pathway  Pathway Diagram
 Glycosaminoglycan Biosynthesis - Keratan Sulfate Pathway antibodies  Glycosaminoglycan Biosynthesis - Keratan Sulfate Pathway Diagram
 Glycosaminoglycan Biosynthesis - Keratan Sulfate Pathway antibodies  Glycosaminoglycan Biosynthesis - Keratan Sulfate Pathway Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >2 publications with CHST2 and Neoplasms
 Lymphoma Antibodies  >2 publications with CHST2 and Lymphoma
 Drug Toxicity Antibodies  >2 publications with CHST2 and Drug Toxicity
 Inflammation Antibodies  >2 publications with CHST2 and Inflammation
 Necrosis Antibodies  >2 publications with CHST2 and Necrosis
 Lung Neoplasms Antibodies  >1 publications with CHST2 and Lung Neoplasms
 Pain Antibodies  >1 publications with CHST2 and Pain
 Precursor Cell Lymphoblastic Leukemia-Lymphoma Antibodies  >1 publications with CHST2 and Precursor Cell Lymphoblastic Leukemia-Lymphoma
 Osteosarcoma Antibodies  >1 publications with CHST2 and Osteosarcoma
 Lymphatic Diseases Antibodies  >1 publications with CHST2 and Lymphatic Diseases
Organs/Tissues associated with CHST2 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >4 publications with CHST2 and Bone
 Other Antibodies  >2 publications with CHST2 and Other
 Brain Antibodies  >2 publications with CHST2 and Brain
 Joint Antibodies  >1 publications with CHST2 and Joint
 Lung Antibodies  >1 publications with CHST2 and Lung
 Embryonic Tissue Antibodies  >1 publications with CHST2 and Embryonic Tissue
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