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CA9 elisa kit :: Mouse carbonic anhydrase IX ELISA Kit

Scan QR to view Datasheet Catalog #    MBS939749 CA9 elisa kit
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

carbonic anhydrase IX (CA9), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Carbonic anhydrase 9, CA9 ELISA Kit

 Product Synonym Names    Mouse Carbonic anhydrase 9 (CA9) ELISA kit; CAIX; MN; RCC-associated protein G250; carbonic dehydratase; carbonic anhydrase IX
 Product Gene Name   

CA9 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for Q8VHB5
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse CA9. No significant cross-reactivity or interference between mouse CA9 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, cell lysates
 Assay Type    Quantitative Sandwich
 Detection Range    0.156 ng/ml -10 ng/ml
 Sensitivity    Typically less than 0.039 ng/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CA9 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CA9 purchase    MBS939749 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the carbonic anhydrase IX (CA9) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156 ng/ml -10 ng/ml in biological research samples containing CA9, with an estimated sensitivity of Typically less than 0.039 ng/ml. The ELISA analytical biochemical technique of the MBS939749 kit is based on CA9 antibody-CA9 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CA9 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CA9. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CA9 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CA9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CA9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CA9 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CA9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Sample Manual Insert of MBS939749. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CA9. It may not necessarily be applicable to this product.
 NCBI GI #    145046220
 NCBI GeneID    230099
 NCBI Accession #    NP_647466.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_139305.2 [Other Products]
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 UniProt Primary Accession #    Q8VHB5 [Other Products]
 UniProt Secondary Accession #    Q8K1G1; Q8VDE4 [Other Products]
 UniProt Related Accession #    Q8VHB5 [Other Products]
 Molecular Weight    47,265 Da
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 NCBI Official Full Name    carbonic anhydrase 9
 NCBI Official Synonym Full Names    carbonic anhydrase 9
 NCBI Official Symbol    Car9 [Similar Products]
 NCBI Official Synonym Symbols   
Ca9; CAIX; MN/CA9
[Similar Products]
 NCBI Protein Information    carbonic anhydrase 9; CA-IX; carbonic anhydrase IX; carbonate dehydratase IX; membrane antigen MN homolog
 UniProt Protein Name    Carbonic anhydrase 9
 UniProt Synonym Protein Names   
Carbonate dehydratase IX; Carbonic anhydrase IX; CA-IX; CAIX; Membrane antigen MN homolog
 Protein Family    Carbonic anhydrase
 UniProt Gene Name    Ca9 [Similar Products]
 UniProt Synonym Gene Names    Car9; CA-IX; CAIX [Similar Products]
 UniProt Entry Name    CAH9_MOUSE
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 UniProt Comments for CA9    CA9: Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. Forms oligomers linked by disulfide bonds. By hypoxia. Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa. Inhibited by coumarins, saccharin, sulfonamide derivatives such as acetazolamide (AZA) and Foscarnet (phosphonoformate trisodium salt). Belongs to the alpha-carbonic anhydrase family.

Protein type: Energy Metabolism - nitrogen; Nucleolus; Membrane protein, integral; Lyase; EC 4.2.1.1

Cellular Component: cell projection; membrane; basolateral plasma membrane; integral to membrane; plasma membrane

Molecular Function: carbonate dehydratase activity; zinc ion binding; metal ion binding; lyase activity

Biological Process: secretion; morphogenesis of an epithelium; one-carbon compound metabolic process
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 Research Articles on CA9    1. Downregulation of carbonic anhydrase IX promotes Col10a1 expression in chondrocytes.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with CA9 elisa kitOrgans/Tissues associated with CA9 elisa kit
 Products by Pathway  Pathway Diagram
 Cellular Response To Hypoxia Pathway antibodies  Cellular Response To Hypoxia Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Regulation Of Gene Expression By Hypoxia-inducible Factor Pathway antibodies  Regulation Of Gene Expression By Hypoxia-inducible Factor Pathway Diagram
 Regulation Of Hypoxia-inducible Factor (HIF) By Oxygen Pathway antibodies  Regulation Of Hypoxia-inducible Factor (HIF) By Oxygen Pathway Diagram
 Reversible Hydration Of Carbon Dioxide Pathway antibodies  Reversible Hydration Of Carbon Dioxide Pathway Diagram
 Organ/Tissue Name  Pubmed Publications
 Stomach Antibodies  >3 publications with CA9 and Stomach
 Kidney Antibodies  >1 publications with CA9 and Kidney
 Intestine Antibodies  >1 publications with CA9 and Intestine
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