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CHIT1 elisa kit :: Mouse chitinase 1 (chitotriosidase) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS937536
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

chitinase 1 (chitotriosidase) (CHIT1), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Chitotriosidase-1, CHIT1 ELISA Kit

 Product Synonym Names    Mouse Chitotriosidase-1 (CHIT1) ELISA kit; CHI3; CHIT; FLJ00314; MGC125322; chitotriosidase; plasma methylumbelliferyl tetra-N-acetylchitotetraoside hydrolase; chitinase 1 (chitotriosidase)
 Product Gene Name   

CHIT1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS937536 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q9D7Q1
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Mouse CHIT1. No significant cross-reactivity or interference between Mouse CHIT1 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    15.6 pg/ml-1000 pg/ml
 Sensitivity    3.9 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Biosynthesis/Metabolism
 Protein Biological Process 2    glyconeogenesis and glycometabolism
 Protein Biological Process 3    Carbohydrate metabolism
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CHIT1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CHIT1 purchase    MBS937536 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the chitinase 1 (chitotriosidase) (CHIT1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.6 pg/ml-1000 pg/ml in biological research samples containing CHIT1, with an estimated sensitivity of 3.9 pg/ml. The ELISA analytical biochemical technique of the MBS937536 kit is based on CHIT1 antibody-CHIT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CHIT1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CHIT1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CHIT1 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CHIT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CHIT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CHIT1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHIT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of CHIT1 elisa kit    CHIT1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS937536. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CHIT1. It may not necessarily be applicable to this product.
 NCBI GI #    548961918
 NCBI GeneID    71884
 NCBI Accession #    NP_001271453.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001284524.1 [Other Products]
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 UniProt Primary Accession #    Q9D7Q1 [Other Products]
 UniProt Secondary Accession #    Q6QJD2 [Other Products]
 UniProt Related Accession #    Q9D7Q1 [Other Products]
 Molecular Weight    51,112 Da
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 NCBI Official Full Name    chitotriosidase-1 isoform 2
 NCBI Official Synonym Full Names    chitinase 1 (chitotriosidase)
 NCBI Official Symbol    Chit1 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    chitotriosidase-1; chitinase-1
 UniProt Protein Name    Chitotriosidase-1
 UniProt Synonym Protein Names   
 Protein Family    Chitotriosidase
 UniProt Gene Name    Chit1 [Similar Products]
 UniProt Entry Name    CHIT1_MOUSE
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 UniProt Comments for CHIT1    CHIT1: Degrades chitin, chitotriose and chitobiose. May participate in the defense against nematodes and other pathogens. Isoform 3 has no enzymatic activity. Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Carbohydrate Metabolism - amino sugar and nucleotide sugar; EC; Hydrolase

Cellular Component: extracellular space; lysosome; extracellular region

Molecular Function: endochitinase activity; hydrolase activity; hydrolase activity, acting on glycosyl bonds; chitin binding; chitinase activity; hydrolase activity, hydrolyzing O-glycosyl compounds

Biological Process: polysaccharide catabolic process; metabolic process; carbohydrate metabolic process; immune response; chitin catabolic process; chitin metabolic process
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 Research Articles on CHIT1    1. CHIT1 exerts protective effects against atherosclerosis by suppressing inflammatory responses and polarizing macrophages toward an M2 phenotype, and promoting lipid uptake and cholesterol efflux in macrophages.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with CHIT1 elisa kitDiseases associated with CHIT1 elisa kit
 Products by Pathway  Pathway Diagram
 Amino Sugar And Nucleotide Sugar Metabolism Pathway antibodies  Amino Sugar And Nucleotide Sugar Metabolism Pathway Diagram
 Amino Sugar And Nucleotide Sugar Metabolism Pathway antibodies  Amino Sugar And Nucleotide Sugar Metabolism Pathway Diagram
 Disease Name  Pubmed Publications
 Gaucher Disease Antibodies  >4 publications with CHIT1 and Gaucher Disease
 Neoplasms Antibodies  >3 publications with CHIT1 and Neoplasms
 Gastritis Antibodies  >1 publications with CHIT1 and Gastritis
 Leukemia, Myeloid Antibodies  >1 publications with CHIT1 and Leukemia, Myeloid
 Necrosis Antibodies  >1 publications with CHIT1 and Necrosis
 Cardiovascular Diseases Antibodies  >1 publications with CHIT1 and Cardiovascular Diseases
Organs/Tissues associated with CHIT1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >3 publications with CHIT1 and Lung
 Tongue Antibodies  >1 publications with CHIT1 and Tongue
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