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CLIC1 elisa kit :: Mouse chloride intracellular channel 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS926340
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

chloride intracellular channel 1 (CLIC1), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Chloride intracellular channel protein 1, CLIC1 ELISA Kit

 Product Synonym Names    Mouse Chloride intracellular channel protein 1 (CLIC1) ELISA kit; DADB-110M10.1; G6; NCC27; OTTHUMP00000029133; OTTHUMP00000029137; OTTHUMP00000174486; RNCC protein; chloride channel ABP; nuclear chloride ion channel protein; p64CLCP; chloride intracellular channel 1
 Product Gene Name   

CLIC1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS926340 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q9Z1Q5
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse CLIC1. No significant cross-reactivity or interference between mouse CLIC1 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    12.5 pg/ml -800 pg/ml.
 Sensitivity    The minimum detectable dose of mouse CLIC1 is typically less than 3.12 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CLIC1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CLIC1 purchase    MBS926340 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the chloride intracellular channel 1 (CLIC1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 12.5 pg/ml -800 pg/ml in biological research samples containing CLIC1, with an estimated sensitivity of The minimum detectable dose of mouse CLIC1 is typically less than 3.12 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS926340 kit is based on CLIC1 antibody-CLIC1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CLIC1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CLIC1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CLIC1 elisa kit

   Princple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CLIC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CLIC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CLIC1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CLIC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of CLIC1 elisa kit    CLIC1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS926340. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CLIC1. It may not necessarily be applicable to this product.
 NCBI GI #    15617203
 NCBI GeneID    114584
 NCBI Accession #    NP_254279.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_033444.2 [Other Products]
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 UniProt Primary Accession #    Q9Z1Q5 [Other Products]
 UniProt Related Accession #    Q9Z1Q5 [Other Products]
 Molecular Weight    27,013 Da
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 NCBI Official Full Name    chloride intracellular channel protein 1
 NCBI Official Synonym Full Names    chloride intracellular channel 1
 NCBI Official Symbol    Clic1 [Similar Products]
 NCBI Official Synonym Symbols   
G6; Clcp
[Similar Products]
 NCBI Protein Information    chloride intracellular channel protein 1; NCC27; nuclear chloride ion channel 27
 UniProt Protein Name    Chloride intracellular channel protein 1
 UniProt Synonym Protein Names   
Nuclear chloride ion channel 27; NCC27
 Protein Family    Chloride intracellular channel protein
 UniProt Gene Name    Clic1 [Similar Products]
 UniProt Synonym Gene Names    NCC27 [Similar Products]
 UniProt Entry Name    CLIC1_MOUSE
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 UniProt Comments for CLIC1    CLIC1: Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Involved in regulation of the cell cycle. Belongs to the chloride channel CLIC family.

Protein type: Membrane protein, integral; Channel, chloride

Cellular Component: extracellular space; mitochondrion; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; integral to membrane; nuclear envelope; nucleus; vesicle

Molecular Function: chloride channel activity; voltage-gated ion channel activity

Biological Process: positive regulation of osteoblast differentiation; regulation of mitochondrial membrane potential; regulation of cell cycle; transport; ion transport; chloride transport
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 Research Articles on CLIC1    1. These data all point to an important role for CLIC1 in regulating macrophage function through its ion channel activity.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with CLIC1 elisa kitOrgans/Tissues associated with CLIC1 elisa kit
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >13 publications with CLIC1 and Neoplasms
 Adenocarcinoma Antibodies  >7 publications with CLIC1 and Adenocarcinoma
 Nervous System Diseases Antibodies  >4 publications with CLIC1 and Nervous System Diseases
 Liver Diseases Antibodies  >4 publications with CLIC1 and Liver Diseases
 Brain Diseases Antibodies  >4 publications with CLIC1 and Brain Diseases
 Necrosis Antibodies  >4 publications with CLIC1 and Necrosis
 Liver Neoplasms Antibodies  >4 publications with CLIC1 and Liver Neoplasms
 Neoplasm Metastasis Antibodies  >4 publications with CLIC1 and Neoplasm Metastasis
 Inflammation Antibodies  >3 publications with CLIC1 and Inflammation
 Ischemia Antibodies  >2 publications with CLIC1 and Ischemia
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >7 publications with CLIC1 and Brain
 Blood Antibodies  >6 publications with CLIC1 and Blood
 Muscle Antibodies  >5 publications with CLIC1 and Muscle
 Kidney Antibodies  >5 publications with CLIC1 and Kidney
 Bone Antibodies  >4 publications with CLIC1 and Bone
 Liver Antibodies  >4 publications with CLIC1 and Liver
 Stomach Antibodies  >2 publications with CLIC1 and Stomach
 Intestine Antibodies  >2 publications with CLIC1 and Intestine
 Lung Antibodies  >2 publications with CLIC1 and Lung
 Ovary Antibodies  >2 publications with CLIC1 and Ovary
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