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CHRM5 elisa kit :: Mouse cholinergic receptor, muscarinic 5 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS930610
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

cholinergic receptor, muscarinic 5 (CHRM5), ELISA Kit

 Also Known As   

Mouse Muscarinic acetylcholine receptor M5, CHRM5 ELISA Kit

 Product Synonym Names    Mouse Muscarinic acetylcholine receptor M5 (CHRM5) ELISA kit; HM5; MGC41838; muscarinic acetylcholine receptor M5; cholinergic receptor; muscarinic 5
 Product Gene Name   

CHRM5 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 MBS930610 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q920H4
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse CHRM5. No significant cross-reactivity or interference between mouse CHRM5 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    31.25 pg/ml -2000 pg/ml.
 Sensitivity    The minimum detectable dose of mouse CHRM5 is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of CHRM5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CHRM5 purchase    MBS930610 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the cholinergic receptor, muscarinic 5 (CHRM5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25 pg/ml -2000 pg/ml in biological research samples containing CHRM5, with an estimated sensitivity of The minimum detectable dose of mouse CHRM5 is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS930610 kit is based on CHRM5 antibody-CHRM5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CHRM5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CHRM5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for CHRM5 elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CHRM5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CHRM5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CHRM5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHRM5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of CHRM5 elisa kit    CHRM5 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS930610
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NCBI/Uniprot data below describe general gene information for CHRM5. It may not necessarily be applicable to this product.
 NCBI GI #    254675187
 NCBI GeneID    213788
 NCBI Accession #    NP_991352.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_205783.2 [Other Products]
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 UniProt Primary Accession #    Q920H4 [Other Products]
 UniProt Secondary Accession #    Q0VBI9; A2AHU3 [Other Products]
 UniProt Related Accession #    Q920H4 [Other Products]
 Molecular Weight    60,253 Da
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 NCBI Official Full Name    muscarinic acetylcholine receptor M5
 NCBI Official Synonym Full Names    cholinergic receptor, muscarinic 5
 NCBI Official Symbol    Chrm5 [Similar Products]
 NCBI Official Synonym Symbols   
m5; M5R; AChR-M5
[Similar Products]
 NCBI Protein Information    muscarinic acetylcholine receptor M5; muscarinic acetylcholine receptor 5
 UniProt Protein Name    Muscarinic acetylcholine receptor M5
 Protein Family    Muscarinic acetylcholine receptor
 UniProt Gene Name    Chrm5 [Similar Products]
 UniProt Entry Name    ACM5_MOUSE
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 UniProt Comments for CHRM5    mAChR M5: The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM5 sub-subfamily.

Protein type: GPCR, family 1; Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass

Cellular Component: postsynaptic membrane; membrane; integral to plasma membrane; integral to membrane; plasma membrane; synapse; cell junction

Molecular Function: G-protein coupled receptor activity; signal transducer activity; extracellular-glycine-gated chloride channel activity; G-protein coupled acetylcholine receptor activity

Biological Process: gastric acid secretion; dopamine transport; G-protein coupled receptor protein signaling pathway; synaptic transmission; acetylcholine receptor signaling, muscarinic pathway; transmission of nerve impulse; signal transduction
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 Research Articles on CHRM5    1. This study demonistrated that activation of M5 receptors in the striatum induces an inhibition in dopamine release.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with CHRM5 elisa kitDiseases associated with CHRM5 elisa kit
 Products by Pathway  Pathway Diagram
 Amine Ligand-binding Receptors Pathway antibodies  Amine Ligand-binding Receptors Pathway Diagram
 Calcium Regulation In The Cardiac Cell Pathway antibodies  Calcium Regulation In The Cardiac Cell Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Cholinergic Synapse Pathway antibodies  Cholinergic Synapse Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies  Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >2 publications with CHRM5 and Nervous System Diseases
 Fibrosis Antibodies  >1 publications with CHRM5 and Fibrosis
 Pain Antibodies  >1 publications with CHRM5 and Pain
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