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CLDN5 elisa kit :: Mouse claudin 5 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS903469
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

claudin 5 (CLDN5), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Claudin-5, CLDN5 ELISA Kit

 Product Synonym Names    Mouse Claudin-5 (CLDN5) ELISA kit; AWAL; BEC1; CPETRL1; TMVCF; OTTHUMP00000197372; OTTHUMP00000197819; androgen withdrawal and apoptosis induced protein RVP1-like; transmembrane protein deleted in velocardiofacial syndrome; claudin 5
 Product Gene Name   

CLDN5 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS903469 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for O54942
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse CLDN5. No significant cross-reactivity or interference between mouse CLDN5 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, cell lysates
 Assay Type    Quantitative Sandwich
 Detection Range    18.75 pg/ml -1200 pg/ml
 Sensitivity    4.68 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CLDN5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CLDN5 purchase    MBS903469 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the claudin 5 (CLDN5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 18.75 pg/ml -1200 pg/ml in biological research samples containing CLDN5, with an estimated sensitivity of 4.68 pg/ml. The ELISA analytical biochemical technique of the MBS903469 kit is based on CLDN5 antibody-CLDN5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CLDN5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CLDN5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CLDN5 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CLDN5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CLDN5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CLDN5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CLDN5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of CLDN5 elisa kit    CLDN5 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS903469. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CLDN5. It may not necessarily be applicable to this product.
 NCBI GI #    31980735
 NCBI GeneID    12741
 NCBI Accession #    NP_038833.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_013805.4 [Other Products]
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 UniProt Primary Accession #    O54942 [Other Products]
 UniProt Secondary Accession #    O88789 [Other Products]
 UniProt Related Accession #    O54942 [Other Products]
 Molecular Weight    23,054 Da
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 NCBI Official Full Name    claudin-5
 NCBI Official Synonym Full Names    claudin 5
 NCBI Official Symbol    Cldn5 [Similar Products]
 NCBI Official Synonym Symbols   
MBEC1; Tmvcf; AI854493
[Similar Products]
 NCBI Protein Information    claudin-5; lung-specific membrane protein; transmembrane protein deleted in VCFS; brain endothelial cell clone 1 protein
 UniProt Protein Name    Claudin-5
 UniProt Synonym Protein Names   
Brain endothelial cell clone 1 protein; Lung-specific membrane protein
 Protein Family    Claudin
 UniProt Gene Name    Cldn5 [Similar Products]
 UniProt Synonym Gene Names    Bec1 [Similar Products]
 UniProt Entry Name    CLD5_MOUSE
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 NCBI Summary for CLDN5    This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. The protein encoded by this gene is a critical component of endothelial tight junctions that control pericellular permeability. The knockout mice lacking this gene died within 10 h of birth and the blood-brain barrier in these mice against small molecules was selectively affected. This gene is expressed strongly in endothelium of normal lung and plays a regulation role during acrolein-induced acute lung injury. [provided by RefSeq, Aug 2010]
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 UniProt Comments for CLDN5    Claudin-5: Plays a major role in tight junction-specific obliteration of the intercellular space. Belongs to the claudin family.

Protein type: Membrane protein, multi-pass; Cytoskeletal; Membrane protein, integral

Cellular Component: tight junction; membrane; paranode region of axon; plasma membrane; integral to membrane; intercellular junction; cell junction

Molecular Function: protein binding; structural molecule activity

Biological Process: myelination; cell-cell adhesion
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 Research Articles on CLDN5    1. silencing of claudin-5 did significantly attenuate simvastatin-mediated endothelial cell barrier protection in response to thrombin
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with CLDN5 elisa kitDiseases associated with CLDN5 elisa kit
 Products by Pathway  Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Circadian Exercise Pathway antibodies  Circadian Exercise Pathway Diagram
 Diurnally Regulated Genes With Circadian Orthologs Pathway antibodies  Diurnally Regulated Genes With Circadian Orthologs Pathway Diagram
 Hepatitis C Pathway antibodies  Hepatitis C Pathway Diagram
 Hepatitis C Pathway antibodies  Hepatitis C Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram
 Tight Junction Pathway antibodies  Tight Junction Pathway Diagram
 Tight Junction Pathway antibodies  Tight Junction Pathway Diagram
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >44 publications with CLDN5 and Nervous System Diseases
 Brain Diseases Antibodies  >42 publications with CLDN5 and Brain Diseases
 Neoplasms Antibodies  >39 publications with CLDN5 and Neoplasms
 Cardiovascular Diseases Antibodies  >32 publications with CLDN5 and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >27 publications with CLDN5 and Disease Models, Animal
 Edema Antibodies  >23 publications with CLDN5 and Edema
 Inflammation Antibodies  >22 publications with CLDN5 and Inflammation
 Cerebral Infarction Antibodies  >16 publications with CLDN5 and Cerebral Infarction
 Stroke Antibodies  >16 publications with CLDN5 and Stroke
 Necrosis Antibodies  >13 publications with CLDN5 and Necrosis
Organs/Tissues associated with CLDN5 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >114 publications with CLDN5 and Brain
 Lung Antibodies  >20 publications with CLDN5 and Lung
 Embryonic Tissue Antibodies  >18 publications with CLDN5 and Embryonic Tissue
 Intestine Antibodies  >10 publications with CLDN5 and Intestine
 Eye Antibodies  >10 publications with CLDN5 and Eye
 Muscle Antibodies  >10 publications with CLDN5 and Muscle
 Liver Antibodies  >6 publications with CLDN5 and Liver
 Pancreas Antibodies  >5 publications with CLDN5 and Pancreas
 Lymph Node Antibodies  >2 publications with CLDN5 and Lymph Node
 Spleen Antibodies  >1 publications with CLDN5 and Spleen
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