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CFP elisa kit :: Mouse complement factor properdin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS935395
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

complement factor properdin (CFP), ELISA Kit

 Also Known As   

Mouse Properdin, CFP ELISA Kit

 Product Synonym Names    Mouse Properdin (CFP) ELISA kit; BFD; PFC; PFD; PROPERDIN; properdin P factor; complement; complement factor properdin
 Product Gene Name   

CFP elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 MBS935395 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P11680
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse CFP. No significant cross-reactivity or interference between mouse CFP and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    1.87 ng/ml -120 ng/ml.
 Sensitivity    The minimum detectable dose of mouse CFP is typically less than 0.46 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of CFP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CFP purchase    MBS935395 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the complement factor properdin (CFP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.87 ng/ml -120 ng/ml in biological research samples containing CFP, with an estimated sensitivity of The minimum detectable dose of mouse CFP is typically less than 0.46 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS935395 kit is based on CFP antibody-CFP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CFP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CFP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for CFP elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CFP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CFP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CFP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CFP bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of CFP elisa kit    CFP elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS935395
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NCBI/Uniprot data below describe general gene information for CFP. It may not necessarily be applicable to this product.
 NCBI GI #    114326449
 NCBI GeneID    18636
 NCBI Accession #    NP_032849.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_008823.3 [Other Products]
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 UniProt Primary Accession #    P11680 [Other Products]
 UniProt Secondary Accession #    Q3TB98; Q3U779 [Other Products]
 UniProt Related Accession #    P11680 [Other Products]
 Molecular Weight    50,327 Da
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 NCBI Official Full Name    properdin
 NCBI Official Synonym Full Names    complement factor properdin
 NCBI Official Symbol    Cfp [Similar Products]
 NCBI Official Synonym Symbols   
Pfc; BCFG
[Similar Products]
 NCBI Protein Information    properdin; complement factor P; properdin factor, complement
 UniProt Protein Name    Properdin
 UniProt Synonym Protein Names   
Complement factor P
 Protein Family    Properdin
 UniProt Gene Name    Cfp [Similar Products]
 UniProt Synonym Gene Names    Pfc [Similar Products]
 UniProt Entry Name    PROP_MOUSE
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 UniProt Comments for CFP    CFP: A positive regulator of the alternate pathway of complement. It binds to and stabilizes the C3- and C5-convertase enzyme complexes. Defects in CFP are the cause of properdin deficiency (PFD). PFD results in higher susceptibility to bacterial infections; especially to meningococcal infections. Three phenotypes have been reported: complete deficiency (type I), incomplete deficiency (type II), and dysfunction of properdin (type III).

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular matrix; extracellular region

Biological Process: immune system process; complement activation, alternative pathway; innate immune response
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 Research Articles on CFP    1. Properdin deficiency exacerbated renal injury in mice lacking complement factor H.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products related to Mouse Properdin, CFP ELISA Kit
 Catalog #  Product Name  Catalog #  Product Name
 MBS9318026  Guinea Pig Properdin, CFP ELISA Kit  MBS145869  Recombinant Human Complement Factor Properdin
 MBS418430  Anti-human complement factor properdin Antibody  MBS935395  Mouse Properdin, CFP ELISA Kit
 MBS934882  Human Properdin, CFP ELISA Kit
Pathways associated with CFP elisa kitDiseases associated with CFP elisa kit
 Products by Pathway  Pathway Diagram
 Herpes Simplex Infection Pathway antibodies  Herpes Simplex Infection Pathway Diagram
 Herpes Simplex Infection Pathway antibodies  Herpes Simplex Infection Pathway Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >407 publications with CFP and Neoplasms
 Mental Disorders Antibodies  >397 publications with CFP and Mental Disorders
 Pain Antibodies  >391 publications with CFP and Pain
 Cardiovascular Diseases Antibodies  >345 publications with CFP and Cardiovascular Diseases
 Respiratory Tract Infections Antibodies  >215 publications with CFP and Respiratory Tract Infections
 Hypertension Antibodies  >170 publications with CFP and Hypertension
 Asthma Antibodies  >109 publications with CFP and Asthma
 Congenital Abnormalities Antibodies  >100 publications with CFP and Congenital Abnormalities
 Breast Neoplasms Antibodies  >89 publications with CFP and Breast Neoplasms
 Respiratory Hypersensitivity Antibodies  >80 publications with CFP and Respiratory Hypersensitivity
Organs/Tissues associated with CFP elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >711 publications with CFP and Blood
 Skin Antibodies  >356 publications with CFP and Skin
 Heart Antibodies  >187 publications with CFP and Heart
 Brain Antibodies  >128 publications with CFP and Brain
 Bone Antibodies  >128 publications with CFP and Bone
 Lung Antibodies  >124 publications with CFP and Lung
 Eye Antibodies  >108 publications with CFP and Eye
 Joint Antibodies  >95 publications with CFP and Joint
 Liver Antibodies  >55 publications with CFP and Liver
 Prostate Antibodies  >53 publications with CFP and Prostate
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